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http://dx.doi.org/10.9721/KJFST.2018.50.4.437

Immunomodulatory effect of bee pollen extract in macrophage cells  

Kim, Yi-Eun (Department of Food Science and Technology, Kongju National University)
Cho, Eun-Ji (Department of Food Science and Technology, Kongju National University)
Byun, Eui-Hong (Department of Food Science and Technology, Kongju National University)
Publication Information
Korean Journal of Food Science and Technology / v.50, no.4, 2018 , pp. 437-443 More about this Journal
Abstract
Activation of macrophages plays an important role in the host-immune system. In this study, we investigated the functional roles and related signaling mechanism of hot-water extracts of bee pollen (BPW) in RAW 264.7 macrophages. Since BPW did not exert cytotoxicity at concentrations ranging from 62.5 to $250{\mu}g/mL$ in macrophage cells, a concentration of $250{\mu}g/mL$ was used as the maximum dose of BPW throughout subsequent experiments. BPW increased inducible nitric oxide synthase-mediated nitric oxide production in a concentration-dependent manner. Additionally, BPW was found to induce macrophage activation by augmenting the expression of cell surface molecules (cluster of differentiation; CD80/86, and major histocompatibility complex; MHC class I/II) and production of pro-inflammatory cytokines (tumor necrosis $factor-{\alpha}$, interleukin-6, and $IL-1{\beta}$) through mitogen-activated protein kinase and nuclear $factor-{\kappa}B$ signaling pathways in RAW 264.7 macrophages. Taken together, our results indicate that BPW could potentially be used as an immunomodulatory agent.
Keywords
bee pollen; macrophage; immuno-modulatory activity; cytokine production; mitogen-activated protein kinase;
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