Browse > Article
http://dx.doi.org/10.9721/KJFST.2014.46.6.729

Anti-inflammatory Activity of an Ethanol Extract of Laminaria japonica Root on Lipopolysaccharide-induced Inflammatory Responses in RAW 264.7 Cells  

Kang, Bo-Kyeong (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kim, Koth-Bong-Woo-Ri (Institute of Fisheries Sciences, Pukyong National University)
Kim, Min-Ji (Institute of Fisheries Sciences, Pukyong National University)
Bark, Si-Woo (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Pak, Won-Min (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kim, Bo-Ram (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Ahn, Na-Kyung (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Choi, Yeon-Uk (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Ahn, Dong-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Publication Information
Korean Journal of Food Science and Technology / v.46, no.6, 2014 , pp. 729-733 More about this Journal
Abstract
Laminaria japonica roots have not been used practically in Korea. In this study, in order to promote the use of these by-products, the anti-inflammatory activity of an ethanol extract of Laminaria japonica root (LJREE) was investigated using a lipopolysaccharide (LPS) to induce an inflammatory response in RAW 264.7 cells. To examine the potential anti-inflammatory effects of LJREE, levels of nitric oxide (NO) and pro-inflammatory cytokines, such as interleukin-6 (IL-6), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), and interleukin-$1{\beta}$ (IL-$1{\beta}$), and cell proliferation were measured. We found that NO levels decreased in a dose-dependent manner, the production of IL-6, TNF-${\alpha}$, and IL-$1{\beta}$ was suppressed, and the production of IL-$1{\beta}$ was inhibited over 30% after treatment with $100{\mu}g/mL$ LJREE. In conclusion, the proliferation of RAW 264.7 cells, measured by MTT assay, confirmed that LJREE may have significant effects on inflammatory factors without any cytotoxicity, making it a potential anti-inflammatory agent.
Keywords
Laminaria japonica root; anti-inflammatory effect;
Citations & Related Records
Times Cited By KSCI : 19  (Citation Analysis)
연도 인용수 순위
1 Oh CK, Oh MC, Kim SH, Lim SB, Kim SH. Antimutagenic and antimicrobial effect of ethanol extracts from sea-mustard and seatangle. J. Korean Fish. Soc. 31: 90-94 (1998)
2 Nakashima H, Kido N, Kobayashi N, Motoki M, Neushul M, Yamamoto N. Purification and characterization of an avian myeloblastosis and human immunodeficiency virus reverse transcriptase inhibitor sulfated polysaccharide extracted from sea algae. Antimicrob. Agents Chemother. 31: 1524-1528 (1987)   DOI   ScienceOn
3 Ryu DS, Oh SM, Kim KH, Kim SH, Choi HJ, Lee DS. Immunomodulating activity of Laminaria japonica polysaccharides. Korean J. Food Sci. Technol. 42: 350-354 (2010)   과학기술학회마을
4 Wang J, Zhang Q, Zhang Z, Song H, Li P. Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica. Int. J. Biol. Macromol. 46: 6-12 (2010)   DOI   ScienceOn
5 Lee JY, Lee MS, Choi HJ, Choi JW, Shin TS, Woo HC, Kim JI, Kim HR. Hexane fraction from Laminaria japonica exerts antiinflammatory effects on lipopolysaccharide-stimulated RAW 264.7 macrophages via inhibiting NF-kappaB pathway. Eur. J. Nutr. 52: 409-421 (2013)   DOI   ScienceOn
6 Chun JY, Cha SH, Lee JS, Kim YS, Park KP. Extraction of carbohydrates and minerals from laminaria using organic acid. Korean Chem. Eng. Res. 50: 238-243 (2012)   과학기술학회마을   DOI
7 Jung HK, Kang BM, Jang JH, Ahn BK, Yeo JH, Jung WS, Cho JH, Kuk YI, Hyun KH, Cho HW. Inhibitory effect of Alopecurus aequalis sobol ethanol extracts on LPS-induced inflammatory response in RAW 264.7 cells. Korean J. Medicinal Crop Sci. 22: 98-104 (2014)   과학기술학회마을   DOI
8 Bhattacharyya A, Pathak S, Datta S, Chattopadhyay S, Basu J, Kundu M. Mitogen-activated protein kinases and nuclear factorkappaB regulate helicobacter pylori-mediated interleukin-8 release from macrophages. Biochem. J. 368: 121-129 (2002)   DOI   ScienceOn
9 Hibi M, Nakajima T, Hirano T. IL-6 cytokine family and signal transduction: a model of the cytokine system. J. Mol. Med. 74: 1-12 (1996)   DOI   ScienceOn
10 Kim HJ, Park TS, Jung MS, Son JH. Study on the anti-oxidant and anti-inflammatory activities of sarcocarp and calyx of persimmon (Cheongdo Bansi). J. Appl. Biol. Chem. 54: 71-78 (2011)   과학기술학회마을   DOI
11 Wang X, Luo Y, Liao WB, Zhang J, Chen TM. Effect of osteoprotegerin in combination with interleukin-6 on inhibition of osteoclast differentiation. Chin. J. Traumatol. 16: 277-280 (2013)
12 Kim YJ, Son DY. Antioxidant and inhibitory effects of korean Panax ginseng extract on pro-inflammatory mediators in LPS-induced RAW 264.7 macrophages. J. Korean Soc. Food Sci. Nutr. 41: 1371-1377 (2012)   DOI   ScienceOn
13 Lee AK, Sung SH, Kim YC, Kim SG. Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-${\alpha}$ and COX-2 expression by sauchinone effects on I-${\kappa}B{\alpha}$ phosphorylation, C/EBP and AP-1 activation. Br. J. Pharmacol. 139: 11-20 (2003)   DOI   ScienceOn
14 Giuliani C, Napolitano G, Bucci I, Montani V, Monaco F. NF-${\kappa}B$ transcription factor: role in the pathogenesis of inflammatory, autoimmune, and neoplastic diseases and therapy implications. Clin. Ter. 152: 249-253 (2001)
15 Medzhitov R. Origin and physiological roles of inflammation. Nature 454: 428-435 (2008)   DOI   ScienceOn
16 Dogne JM, Hanson J, Supuran C, Pratico D. Coxibs and cardiovascular side-effects: from light to shadow. Curr. Pharm. Design 12: 971-975 (2006)   DOI   ScienceOn
17 Makins R, Ballinger A. Gastrointestinal side effects of drugs. Expert Opin. Drug Saf. 2: 421-429 (2003)   DOI
18 Higuchi M, Hisgahi N, Taki H, Osawa T. Cytolytic mechanism of activated macrophages. Tumor necrosis factor and L-arginine-dependent mechanisms act synergistically as the major cytolytic mechanisms of activated macrophages. J. Immunol. 144: 1425-1431 (1990)
19 Ljung T, Lundberg S, Varsanyi M, Johansson C, Schmidt PT, Herufl M, Lundberg JO, Hellstrom PM. Rectal nitric oxide as biomarker in the treatment of inflammatory bowel disease: responders versus nonresponders. World J. Gastroenterol. 12: 3368-3392 (2006)
20 Kim YJ, Son DY. Inflammatory mediator regulation of the Zizyphus jujube leaf fractions in the LPS-stimulated Raw 264.7 mouse macrophage. Korean J. Food Preserv. 21: 114-120 (2014)   DOI
21 Leem HH, Kim EO, Seo MJ, Choi SW. Antioxidant and anti-inflammatory activities of eugenol and its derivatives from clove (Eugenia caryophyllata Thunb.). J. Korean Soc. Food Sci. Nutr. 40: 1361-1370 (2011)   과학기술학회마을   DOI
22 Park YM, Won JH, Yun KJ, Ryu JH, Han YN, Choi SK, Lee KT. Preventive effect of Ginkgo biloba extract (GBB) on the lipopolysaccharide-induced expressions of inducible nitric oxide synthase and cyclooxygenase-2 via suppression nuclear factor-${\kappa}B$ in RAW 264.7 cells. Biol. Pharm. Bull. 29: 985-990 (2006)   DOI   ScienceOn
23 Lee ST, Jeong YR, Ha MH, Kim SH, Byun MW, Jo SK. Induction of nitric oxide and TNF-${\alpha}$ by herbal plant extract in mouse macrophage. J. Korean Soc. Food Sci. Nutr. 29: 342-348 (2000)
24 Choi JS, Shin SH, Ha YM, Kim YC, Kim TB, Park SM, Choi IS, Song HJ, Choi YJ. Mineral contents and physiological activities of dried sea tangle (Laminaria japonica) collected from Gijang and Wando in Korea. Korean J. Life Sci. 18: 474-481 (2008)   과학기술학회마을   DOI
25 Tak PP, Firestein GS. NF-${\kappa}B$: a key role in inflammatory diseases. J. Clin. Invest. 107: 7-11 (2001)   DOI   ScienceOn
26 Delgado AV, McManus AT, Chambers JP. Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin 2, and interleukin 6 by rat leukocyte subpopulations after exposure to substance P. Neuropeptides 37: 355-361 (2003)   DOI   ScienceOn
27 Masters SL, Simon A, Aksentijevich I, Kastner DL. Horror autoinflammaticus: the molecular pathophysiology of autoinflammatory disease. Ann. Rev. Immunol. 27: 621-668 (2009)   DOI   ScienceOn
28 Adams JL, Czuprynski CJ. Mycobacterial cell wall components induce the production of TNF-${\alpha}$, IL-1, and IL-6 by bovine monocytes and the murine macrophage cell line RAW 264.7. Microb. Pathogenesis 16: 401-411 (1994)   DOI   ScienceOn
29 Choi JH, Choi JS, Byun DS, Yang DS. Basic studies on the development of diet for the treatment of obesity. II. Comparison of the inhibitory effect of algae and crude drug components on obesity. Korean J. Fish. Aquat. Sci. 19: 485-492 (1986)   과학기술학회마을
30 Park SG, Jegal KH, Jung JY, Back YD, Byun SH, Kim YW, Cho IJ, Park SM, Kim SC. Leonuri fructus ameliorates acute inflammation via the inhibition of NF-${\kappa}B$-mediated nitric oxide and pro-inflammatory cytokine production. Korean J. Orient. Physiol. Pathol. 28: 178-185 (2014)   과학기술학회마을
31 Weisz A, Cicatiello L, Esumi H. Regulation of the mouse inducible-type nitric oxide synthase gene promoter by interferongamma, bacterial lipopolysaccharide and NG-monomethyl-L-arginine. Biochem. J. 316: 209-215 (1996)
32 Lee YH, Lim EM. Anti-inflammatory effect of Ligustri lucidi fructus water extract in RAW 264.7 cells induced by LPS. J. Korean Obstet. Gynecol. 26: 66-81 (2013)
33 Kang CH, Choi YH, Choi IW, Lee JD, Kim GY. Inhibition of lipopolysaccharide-induced iNOS, COX-2, and TNF-${\alpha}$ expression by aqueous extract of Orixa Japonica in RAW 264.7 cells via suppression of NF-${\kappa}B$ activity. Trop. J. Pharm. Res. 10: 161-168 (2011)
34 Yoon SB, Han HS, Lee YJ. Effect of Scutellariae radix extract on the proinflammatory mediators in RAW 264.7 cells induced by LPS. Korean J. Herbology 26: 75-81 (2011)   과학기술학회마을
35 An SM, Kim HG, Choi EJ, Hwang HH, Lee ES, Baek JH, Boo YC, Koh JS. Screening for anti-inflammatory activities in extracts from Korean herb medicines. J. Soc. Cosmet. Scientists Korea 40: 95-108 (2014)   과학기술학회마을   DOI
36 Miyataka M, Rich KA, Ingram M, Yamamoto T, Bing RJ. Nitric oxide, anti-inflammatory drugs on renal prostaglandins and cyclooxygenase-2. Hypertension 39: 785-789 (2002)   DOI   ScienceOn
37 Moon JH, Go H, Shin SM, Kim KT. Anti-inflammatory effect of extracts from Ligustrum obtusifolium S. fruits in RAW 264.7 macrophages. J. Soc. Korean Med. Diagn. 17: 263-273 (2013)
38 Kim BW, Kim JI, Kim HR, Byun DS. Anti-inflammatory effect of an ethyl acetate fraction from Myagropsis yendoi on lipopolysaccharides-stimulated RAW 264.7 cells. Korean J. Fish. Aquat. Sci. 47: 527-536 (2014)   과학기술학회마을   DOI
39 Iwamoto M, Kurachi M, Nakashima T, Kim DK, Yamaguchi K, Oda T, Iwamoto Y, Muramatsu T. Structure-activity relationship of alginate oligosaccharides in the induction of cytokine production from RAW 264.7 cells. FEBS Lett. 579: 4423-4429 (2005)   DOI   ScienceOn
40 Choi SB, Bae GS, Jo IJ, Park KC, Seo SH, Kim DG, Shin JY, Gwak TS, Lee JH, Lee GS, Park SJ, Song HJ. The anti-inflammatory effect of Lithospermum Erythrorhizon on lipopolysaccharide-induced inflammatory response in RAW 264.7 cells. Korean J. Herbology 28: 67-73 (2013)   과학기술학회마을   DOI
41 Tracey D, Klareskog L, Sasso EH, Salfeld JG, Tak PP. Tumor necrosis factor antagonist mechanisms of action: a comprehensive review. Pharmacol. Ther. 117: 244-279 (2008)   DOI   ScienceOn
42 Lahaye M. Marine algae as sources of fibres: determination of soluble and insoluble dietary fibre contents in some 'sea vegetables'. J. Sci. Food Agr. 54: 587-594 (1991)   DOI
43 Kim YS, Kang CO, Kim MH, Cha WS, Shin HJ. Contents of water extract for Laminaria japonica and its antioxidant activity. Korean Soc. Biotechnol. Bioeng. J. 26: 112-118 (2011)   과학기술학회마을   DOI   ScienceOn
44 Kang KS, Nam CH, Park EK, Ha BJ. The enzymatic regulatory effects of Laminaria japonica fucoidan extract in hepatotoxicity. Korean J. Life Sci. 16: 1104-1108 (2006)   DOI   ScienceOn
45 Kim YY, Lee KW, Kim GB, Cho YJ. Studies on physicochemical and biological properties of depolymerized alginate from sea tangle, Laminaria japonicus by thermal decomposition. J. Korean Fish. Soc. 33: 388-392 (2000)   과학기술학회마을
46 Ryu BH, Kim DS, Cho KJ, Sin DB. Antitumor activity of seaweeds toward sarcoma-180. Korean J. Food Sci. Technol. 21: 595-600 (1989)   과학기술학회마을
47 Vishchuk OS, Ermakova SP, Zvyagintseva TN. Sulfated polysaccharides from brown seaweeds Saccharina japonica and Undaria pinnatifida: isolation, structural characteristics, and antitumor activity. Carbohydr. Res. 346: 2769-2776 (2011)   DOI