Browse > Article
http://dx.doi.org/10.9721/KJFST.2014.46.5.622

Protective Effects of Akebia quinata Fruit Extract on Acute Alcohol-induced Hepatotoxicity in Mice  

Lee, Sang Hoon (Department of Nutritional Science and Food Management, College of Health Science, Ewha Womans University)
Song, Young Sun (Department of Nutritional Science and Food Management, College of Health Science, Ewha Womans University)
Lee, Seo Yeon (Department of Nutritional Science and Food Management, College of Health Science, Ewha Womans University)
Kim, So Young (Department of Nutritional Science and Food Management, College of Health Science, Ewha Womans University)
Ko, Kwang Suk (Department of Nutritional Science and Food Management, College of Health Science, Ewha Womans University)
Publication Information
Korean Journal of Food Science and Technology / v.46, no.5, 2014 , pp. 622-629 More about this Journal
Abstract
We studied the effects of Akebia quinata fruit extract (AQ) on acute alcohol-induced hepatotoxicity in mice. AQ (30-1,000 mg/kg body weight (BW) per day) was orally administered to the study group, once daily for 1 week. On the last day of AQ treatment, ethanol (6 mg/kg BW) was orally administered to induce acute liver injury. The AQ-treated group showed significantly lower levels of alanine aminotransferase and aspartate aminotransferase, compared to the only ethanol-treated group (ETG). The glutathione level in the AQ-treated group elevated up to 20.6%, compared to that observed in the ETG. The mRNA expression of glutathione synthetic enzymes was also higher in the AQ-treated group, compared to the ETG. The AQ-treated group also exhibited lower levels of expression of NADPH oxidase 4 and tumor necrosis factor alpha mRNA. Thus, these results show that AQ treatment can be a potential method to reduce oxidative stress and inflammation in ethanol-treated mouse liver and also that AQ can be a useful therapeutic agent for acute alcohol-induced hepatotoxicity.
Keywords
Akebia quinata; acute alcohol-induced liver injury; glutathione; NADPH oxidase 4; tumor necrosis factor alpha;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Ikuta A, Itokawa H. A triterpene from Akebia quinata callus tissue. Phytochemistry 27: 3809-3810 (1988)   DOI
2 Ellman GL. Tissue sulthydryl groups. Arch. Biochem. Biophys. 82: 70-77 (1959)   DOI   ScienceOn
3 Tietze F. Disulfide reduction in rat liver. I. Evidence for the presence of nonspecific nucleotide-dependent disulfide reductase and GSH-disulfide transhydrogenase activities in the high-speed supernatant fraction. Arch. Biochem. Biophys. 138: 177-188 (1970)   DOI
4 Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal. Biochem. 72: 248-254 (1976)   DOI   ScienceOn
5 Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Methods 25: 402-408 (2001)   DOI   ScienceOn
6 Umulis DM, Gurmen NM, Singh P, Fogler HS. A physiologically based model for ethanol and acetaldehyde metabolism in human beings. Alcohol 35: 3-12 (2005)   DOI   ScienceOn
7 Cha YS, Sachan DS. Acetylcarnitine-mediated inhibition of ethanol oxidation in hepatocytes. Alcohol 12: 289-294 (1995)   DOI   ScienceOn
8 Campbell JS, Hughes SD, Gilbertson DG, Palmer TE, Holdren MS, Haran AC, Odell MM, Bauer RL, Ren HP, Haugen HS, Yeh MM, Fausto N. Platelet-derived growth factor C induces liver fibrosis, steatosis, and hepatocellular carcinoma. P. Natl. Acad. Sci. USA 102: 3389-3394 (2005)   DOI
9 Israel Y, Robert SB, Hector O. Liver cell enlargement induced by chronic alcohol consumption: studies on its causes and consequences. Clin. Biochem.15: 189-192 (1982)
10 Kwo PY, Ramchandani VA, O'connor S, Amann D, Carr LG, Sandrasegaran K, Kopecky KK, Li TK. Gender differences in alcohol metabolism: Relationship to liver volume and effect of adjusting for body mass. Gastroenterology 115: 1552-1557 (1998)   DOI   ScienceOn
11 Gao B, Bataller R, Gastroent. Alcoholic liver disease: Pathogenesis and new therapeutic targets. Gastroenterology. 141: 1572-1585 (2011)   DOI   ScienceOn
12 Stickel F, Seitz HK. Alcoholic steatohepatitis. Best. Pract. Res. Clin. Gastroenterol. 24: 683-693 (2010)
13 Beier JL, McClain CJ, Mechanisms and cell signaling in alcoholic liver disease. J. Biol. Chem. 39: 1249-1264 (2010)
14 Zeng T, Xie KQ. Ethanol and liver: recent advances in the mechanisms of ethanol-induced hepatosteatosis. Arch. Toxicol. 83: 1075-1081 (2009)   DOI
15 Espin N, Lima, V, Lieber CS, Garro AJ. In vitro and in vivo inhibitory effect of ethanol and acetaldehyde on O6-methylguanine transferase. Carcinogenesis 9: 761-766 (1988)   DOI
16 Wondergem R, Davis J. Ethanol increases hepatocyte water volume. Alcohol. Clin. Exp. Res. 18: 1230-1236 (1994)   DOI
17 Ko KS, Yang H, Noureddin M, Iglesia-Ara A, Xia M, Wagner C, Luka Z, Mato JM, Lu SC. Changes in S-adenosylmethionine and GSH homeostasis during endotoxemia in mice. Lab Invest. 88: 1121-1129 (2008)   DOI
18 Lettron P, Fromenty B, Terris B, Degott C, Pessayre D. Acute and chronic hepatic steatosis lead to in vivo lipid peroxidation in mice. J. Hepatol. 24: 200-208(1996)   DOI   ScienceOn
19 Yin M, Wheeler MD, Kono H, Bradford BU, Gallucc RM, Luster MI, Thurman RG. Essential role of tumor necrosis factor alpha in alcohol-induced liver injury in mice. Gastroenterology 117: 942-952 (1999)   DOI   ScienceOn
20 Abdellah M, Demelliers C, Amsellem S, Pessayre D, Fromenty B. Acute ethanol administration oxidatively damages and depletes mitochondrial DNA in mouse liver, brain, heart, and skeletal muscles: protective effects of antioxidants. J. Pharmacol. Exp. Ther. 298: 737-743 (2001)
21 Liu H, Wang H, Shenvi S, Hagen TM, Liu RM. Glutathione metabolism during aging and in Alzheimer disease. Ann. N. Y. Acad. Sci. 1019: 346-349 (2004)   DOI
22 Videla LA, Valenzuela A. Alcohol ingestion, liver glutathione and lipoperoxidation: metabolic interrelations and pathological implications. Life Sci. 31: 2395-2407 (1982)   DOI
23 Sun S, Zhaing H, Xue B, Wu Y, Wang J, Yin Z, Luo L. Protective effect of glutathione against lipopolysaccharide-induced inflammation and mortality in rats. Inflamm. Res. 55: 504-510 (2006)   DOI   ScienceOn
24 Dalton TP, Dieter MZ, Yang Y, Shertzer HG, Nebert DW. Knockout of the mouse glutamate cysteine ligase catalytic subunit (Gclc) gene: embryonic lethal when homozygous, and proposed model for moderate glutathione deficiency when heterozygous. Biochem. Biophys. Res. Commun. 279: 324-329 (2000)   DOI   ScienceOn
25 Lu SC. Regulation of hepatic glutathione synthesis: current concepts and controversies. Faseb. J. 13: 1169-1183 (1999)
26 Vendemiale G, Altomare E, Trizio T, Grazie CL, Padova CD. Salemo MT, Carrieri V, Albano O. Effects of oral S-adenosyl-Lmethionine on hepatic glutathione in patients with liver disease. Scand. J. Gastroenterol. 24: 407-415 (1989)   DOI
27 Vogt BL, Richie JP Jr. Glutathione depletion and recovery after acute ethanol administration in the aging mouse. Biochem. Pharmacol. 73: 1613-1621 (2007)   DOI
28 Bedard K, Krause KH. The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology. Physiol. Rev. 87: 245-313 (2007)   DOI   ScienceOn
29 Medina J, Moreno-Otero R. Pathophysiological basis for antioxidant therapy in chronic liver disease. Drugs 65: 2445-2461 (2005)   DOI   ScienceOn
30 Roskams T, Yang SQ, Koteish A, Durnez A, DeVos R, Huang X, Achten R, Verslype C, Diehl AM. Oxidative stress and oval cell accumulation in mice and humans with alcoholic and nonalcoholic fatty liver disease. Am. J. Pathol. 163: 1301-1311 (2003)   DOI   ScienceOn
31 Jiang JX, Chen X, Serizawa N, Szyndralewiez C, Page P, Schrder K, Brandes RP, Devaraj S, Trk NJ. Liver fibrosis and hepatocyte apoptosis are attenuated by GKT137831, a novel NOX4/NOX1 inhibitor in vivo. Free Radic. Biol. Med. 53: 289-296 (2012)   DOI   ScienceOn
32 Bataller R, Schwabe RF, Choi YH, Yang L, Paik YH, Lindquist J, Qian T, Schoonhoven R, Hagedorn CH, Lemasters JJ, Brenner DA. NADPH oxidase signal transduces angiotensin II in hepatic stellate cells and is critical in hepatic fibrosis. J. Clin. Invest. 112: 1383-1394 (2003)   DOI
33 Zhao XJ, Dong Q, Bindas J, Piganelli JD, Magill A, Reiser J, Kolls JK. TRIF and IRF-3 binding to the TNF promoter results in macrophage TNF dysregulation and steatosis induced by chronic ethanol. J. Immunol. 181: 3049-3056 (2008)   DOI
34 Adachi Y, Moore LE, Bradford BU, Gao W, Thurman RG. Antibiotics prevent liver injury in rats following long-term exposure to ethanol. Gastroenterology 108: 218-224 (1995)   DOI
35 Thurman RG, Bradford BU, Iimuro Y, Knecht KT, Arteel GE, Yin M, Connor HD, Wall C, Raleigh JA, Frankenberg MV, Adachi Y, Forman DT, Brenner D, Kadiiska M, Mason RP. The role of gut-derived bacterial toxins and free radicals in alcoholinduced liver injury. J. Gastroenterol. Hepatol. 13: S39-S50 (1998)
36 Wheeler MD, Nakagami M, Bradford BU, Uesugi T, Mason RP, Connor HD, Dikalova A, Kadiiska M, Thurman RG. Overexpression of manganese superoxide dismutase prevents alcohol-induced liver injury in the rat. J. Biol. Chem. 276: 36664-36672 (2001)   DOI   ScienceOn
37 Ramchandani VA, Bosron WF, Li TK. Research advances in ethanol metabolism. Pathol. Biol. 49: 676-682 (2001)   DOI   ScienceOn
38 Rouach H, Clement M, Ofanelli MT, Janvier B, Nordmann J, Nordmann R. Hepatic lipid peroxidation and mitochondrial susceptibility to peroxidative attacks during ethanol inhalation and withdrawal. Biochem. Biophys. Acta. 753: 439-444 (1983)   DOI   ScienceOn
39 Lu SC, Mato JM. S-Adenosylmethionine in cell growth, apoptosis and liver cancer. J. Gastroenterol. Hepatol. 23: s73-s77 (2008)   DOI   ScienceOn
40 Moncade C, Torres V, Varghese G, Albano E, Israsel Y. Ethanol derived immuno reactive species formed by radical mechanisms. Mol. Phaemacol. 46: 786-791 (1994)
41 Simic MG. Mechanisms of inhibition of free-radical processes in mutagenesis and carcinogensis. Mutat. Res. 202: 377-386 (1988)   DOI   ScienceOn
42 Ikuta A. Saponins and triterpenes from callus tissues of Akebia trifoliate and comparision with the constituents of other Lardizabalaceous callus tissues. J. Nat. Prod. 58: 1378-1383 (1995)   DOI
43 Ji C, Deng Q, Kaplowitz N. Role of TNF-alpha in ethanolinduced hyperhomocysteinemia and murine alcoholic liver injury. Hepatology 40: 442-451 (2004)   DOI   ScienceOn
44 Petrasek J, Dolganiuc A, Csak T, Nath B, Hritz I, Kodys K, Catalano D, Kurt-Jones E, Mandrekar P, Szabo G. Interferon regulatory factor 3 and type I interferons are protective in alcoholic liver injury in mice by way of crosstalk of parenchymal and myeloid cells. Hepatology 53: 649-660 (2011)   DOI
45 McClain CJ, Song Z, Barve SS, Hill DB, Deaciuc I. Recent advances in alcoholic liver disease IV. dysregulated cytokine metabolism in alcoholic liver disease. Am. J. Physiol. Gastrointest. Liver Physiol. 287: 497-502 (2004)   DOI
46 Camandola S, Scavazza A, Leonarduzzi G, Biasi F, Chiarpotto E, Azzi A, Poli G. Biogenic 4-hydroxy-2-nonenal activates transcription factor AP-1 but not NF-kappa B in cells of the macrophage lineage. Biofactors 6: 173-179 (1997)   DOI   ScienceOn
47 Ohshima H, Tazawa H, Sylla BS, Sawa T. Prevention of human cancer by modulation of chronicinflammatory processes. Mutat. Res. 591: 110-122 (2005)   DOI
48 Choi JW, Jung HJ, Lee KT, Park HJ. Antinociceptive and antiinflammatory effects of the saponin and sapogenins obtained from the stem of Akebia quinata. J. Med. Food 8: 78-85 (2005)   DOI   ScienceOn
49 Higuchi R, Kawasaki T. Pericarp saponins of Akebia quinata Decne. I. Glycosides of hederagenin and oleanolic acid. Chem. Pharm. Bull. 24: 1021-1032 (1976)   DOI
50 Kang SH, Kang JS, Jeong WS. Cytotoxic and apoptotic effects of saponins from Akebia quinata on HepG2 hepatocarcinoma cells. Korean. J. Food. Preserv. 17: 311-319 (2010)   과학기술학회마을
51 Mato JM, Cmara J, Fernndez de Paz J, Caballera L, Coll S, Caballero A, Garca-Buey L, Beltrn J, Benita V, Caballera J, Sol R, Moreno-Otero R, Barrao F, Martn-Duce A, Correa JA, Pars A, Barrao E, Garca-Magaz I, Puerta JL, Moreno J, Boissard G, Ortiz P, Rods J. S-adenosylmethionine in alcoholic liver cirrhosis: a randomized, placebo- controlled, double-blind, multicenter clinical trial. J. Hepatol. 30: 1081-1089 (1999)   DOI   ScienceOn
52 Jung HJ, Lee CO, Lee TK, Choi JW, Park HJ. Structure - activity relationship of oleanane disaccharides isolated from Akebia quinata versus cytotoxicity against cancer cells and NO inhibition. Biol. Pharm. Bull. 27: 744-747 (2004)   DOI   ScienceOn
53 Degracea P, Demizieuxa L, Gresti J, Chardigny JM, Sbdio JL, Clouet P. Association of liver steatosis with lipid oversecretion and hypotriglyceridaemia in C57BL/6j mice fed trans-10, cis-12- linoleic acid. FEBS. Lett. 546: 335-339 (2003)   DOI