Browse > Article
http://dx.doi.org/10.9721/KJFST.2014.46.5.609

Nutritional Composition and Cytoprotective Effect of Moringa oleifera Lam.  

Jin, Su Il (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University)
Kim, Hyeon Ju (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University)
Jeong, Ji Hee (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University)
Jin, Dong Eun (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University)
Choi, Sung-Gil (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University)
Heo, Ho Jin (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University)
Publication Information
Korean Journal of Food Science and Technology / v.46, no.5, 2014 , pp. 609-615 More about this Journal
Abstract
The cytoprotective effect of Moringa oleifera Lam. (drumstick tree) on neuronal cells was investigated to confirm the physiological benefits associated with this natural food resource. First, the drumstick tree extract was chemically analyzed to determine inherent nutritional constituents. Calcium and potassium were identified as the major mineral constituents, and palmitic acid (C16:0, 16.33%) and gadoleic acid (C20:01, 66.34%) were detected as the major fatty acids. Moreover, drumstick tree extract contained 94.78 mg/100 g vitamin E and 112.61 mg/100 g niacin. PC12 cells were used to study the cytoprotective effects of drumstick tree extract. Intracellular accumulation of reactive oxygen species was significantly reduced when $H_2O_2$ treated-neuronal cells were cultured in a medium containing the methanolic extract of drumstick tree, compared to cells treated with only $H_2O_2$. Cell viability assay using MTT showed that the extract protected cells against $H_2O_2$-induced neurotoxicity and inhibited LDH leakage from the cell membrane. Caspase assay showed that the extract exerted cytoprotective effect against apoptosis. Consequently, these data suggest that drumstick tree is a useful natural resource with positive effects on human health.
Keywords
Moringa oleifera Lam; drumstick tree; PC12 cell;
Citations & Related Records
Times Cited By KSCI : 11  (Citation Analysis)
연도 인용수 순위
1 Kwon SH, Kim KN, Shim JS, Kim YH. Statistics, KNHANESIV. pp. 79-297. In: The Forth Korean National Health and Nutrition Survey 2007-First Year Report. Korean Health Industry Development Institute/Ministry of Health and Welfare. Cheongju, Korea (2009)
2 Chang SO, Bae SK. Development of high calcium dishes for elementary school lunch and perception on calcium supply by school dietitian. J. Korean Soc. Food Sci. Nutr. 38: 1373-1380 (2009)   과학기술학회마을   DOI
3 Sengev AI, Abu JO, Gernah DI. Effect of Moringa oleifera leaf powder supplementation on some quality characteristics of wheat bread. Food Nutr. Sci. 4:270-275 (2013)   DOI
4 Staprans I, Rapp JH, Pan XM, Feingold K. The effect of oxidized lipids in the diet on serum lipoprotein peroxides in control and diabetic rats. J. Clin. Invest. 92: 638-643 (1993)   DOI   ScienceOn
5 Choi MJ, Kim HK, Lee MS. Vitamin E in vivo studies on the activity of antioxidant enzymes and CYP2E1 expression in high PUFA-treated brains. J. Korean Soc. Food Sci. Nutr. 41: 1106-1111 (2012)   과학기술학회마을   DOI
6 Hannan MA, Kang JY, Mohibbullah M, Hong YK, Lee HS, Choi JS, Choi IS, Moon IS. Moringa oleifera with promising neuronal survival and neurite outgrowth promoting potentials. J. Ethnopharmacol. 152: 142-150 (2014)   DOI
7 Vongsak B, Sithisarn P, Mangmool S, Thongpraditchote S, Wongkrajang Y, Gritsanapan W. Maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringa oleifera leaf extract by the appropriate extraction method. Ind. Crop. Prod. 44: 566-571 (2013)   DOI
8 Edmondson JM, Armstrong LS, Martinez AO. A rapid and simple MTT-based spectrophotometric assay for determining drug sensitivity in monolayer cultures. J. Tissue Cult. Method. 11: 15-17 (1988)   DOI   ScienceOn
9 Vegran F, Boidot R, Oudin C, Riedinger JM, Lizard-Nacol S. Implication of alternative splice transcripts of caspase-3 and survivin in chemoresistance. Bull. Cancer 92: 219-226 (2005)
10 Donovan M, Cotter TG. Control of mitochondrial integrity by Bcl-2 family members and caspase-independent cell death. Biochim. Biophys. Acta. 1644: 133-147 (2004)   DOI   ScienceOn
11 Yoon MY, Lee HJ, Lee BB, Lee SM,. Kim JY, Kim Y, Park E, Park HR. Protective effect of Schizonepeta tenuifolia Briquet extracts on oxidative DNA damage in human leucocytes and on hydrogen peroxide-induced cytotoxicity in PC12 cells. Food Sci. Biotechnol. 16: 858-862 (2007)   과학기술학회마을
12 Um MY, Ha TY, Seong KS, Kim YS. In vitro screening of the acetylcholinesterase inhibition, antioxidant activity, and neuronal cell protective effect of medicinal plant extracts. Korean J. Food Preserv. 20: 840-845 (2013)   과학기술학회마을   DOI
13 Yoon MY, Kim JY, Hwang JH, Cha MR, Jo KJ, Park HR. Protective effect of methanolic extracts from Dendrobium nobile Lindl. on $H_2O_2$-induced neurotoxicity in PC12 cells. J. Korean Soc. Appl. Chem. 50: 63-67 (2007)   과학기술학회마을
14 Cho IY, Sheen YY. Effect of dioxin on the change of mitochondrial inner membrane potential and the induction of ROS. J. Environ. Toxicol. 24: 33-41 (2009)   과학기술학회마을
15 Staples G, Herbst DR. A tropical garden flora: plants cultivated in the Hawaiian Islands and other tropical places. Bishop Museum Press. Honolulu, HI, USA. p. 908 (2005)
16 Jeong EJ, Sung SH, Kim J, Kim SH, Kim YC. Rhus verniciflua stokes attenuates glutamate-induced neurotoxicity in primary cultures of rat cortical cells. Nat. Prod. Sci. 14: 156-160 (2008)   과학기술학회마을
17 Parfenova H, Basuroy S, Bhattacharya S, Tcheranova D, Qu Y, Regan RF, Leffler CW. Glutamate induces oxidative stress and apoptosis in cerebral vascular endothelial cells: contributions of HO-1 and HO-2 to cytoprotection. Am. J. Physiol. Cell Physiol. 290: 1399-1410 (2006)
18 Ha JS, Park SS. Glutamate-induced oxidative stress, but not cell death, is largely dependent upon extracellular calcium in mouse neuronal HT22 cells. Neurosci. Lett. 393: 165-169 (2006)   DOI
19 Anwar F, Latif S, Ashraf M, Gilani AH. Moringa oleifera: a food plant with multiple medicinal uses. Phytother. Res. 21: 17-25 (2007)   DOI   ScienceOn
20 Bharali R, Tabassum J, Azad MR. Chemomodulatory effect of Moringa oleifera, Lam, on hepatic carcinogen metabolising enzymes, antioxidant parameters and skin papillomagenesis in mice. Asian Pac. J. Cancer Prev. 4: 131-139 (2003)
21 Siddhuraju, P, Becker, K. Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick-tree (Moringa oleifera Lam.) leaves. J. Agr. Food Chem. 51: 2144-2155 (2003)   DOI   ScienceOn
22 Hamza AA. Ameliorative effects of Moringa oleifera Lam seed extract on liver fibrosis in rats. Food Chem. Toxicol. 48: 345-355 (2010)   DOI
23 Jeong CH, Bae YI, Lee HJ, Shim KH. Chemical components of propolis and its ethanolic extracts. J. Korean Soc. Food Sci. Nutr. 32: 501-505 (2003)   과학기술학회마을   DOI
24 Ha JH, Shim YS, Seo HY, Nam HJ, Masahito I, Hiroaki N. Rapid method for determination of $\beta$-carotene in foods using ultra high performance liquid chromatography. Food Sci. Biotechnol. 19: 1199-1204 (2010)   과학기술학회마을   DOI
25 Sreelatha S, Jeyachitra A, Padma PR. Antiproliferation and induction of apoptosis by Moringa oleifera leaf extract on human cancer cells. Food Chem. Toxicol. 6: 1270-1275 (2011)
26 Metcalfe LD, Schmitz AA, Pelka JR. Rapid preparation of fatty acid esters form lipids for gas chromatographic analysis. Anal. Chem. 38: 514-515 (1966)   DOI
27 Kim HS, Jang DK, Woo DK, Woo KL. Comparision of preparation methods for water soluble vitamin analysis in foods by reversed-phase high performance liquid chromatography. Korean J. Food Sci. Technol. 34: 141-150 (2002)   과학기술학회마을
28 Kwak BM, Lee KW, Ahn JH, Kong UY. Simultaneous determination of vitamin A and E in infant formula by rapid extraction and HPLC with photodiode array detection. Korean J. Food Sci. Technol. 36: 189-195 (2004)   과학기술학회마을
29 Heo HJ, Cho HY, Hong BS, Kim HK, Kim EK, Kim BK, Shin, DH. Protective effect of 4',5-dihydroxy-3',6,7-trimethoxyflavone from Artemisia asiatica against $A{\beta}$-induced oxidative stress in PC12 cells. Amyloid. 8: 194-201 (2001)   DOI   ScienceOn