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http://dx.doi.org/10.9721/KJFST.2014.46.4.489

Effects of Unripe Black Raspberry Water Extract on Lipid Metabolism and Oxidative Stress in Mice  

Choi, Hye Ran (Gochang Black Raspberry Research Institute)
Lee, Jung-Hyun (Gochang Black Raspberry Research Institute)
Lee, Su Jung (Gochang Black Raspberry Research Institute)
Lee, Min Jung (Gochang Black Raspberry Research Institute)
Jeong, Jong Tae (Gochang Black Raspberry Research Institute)
Lee, Tae-Bum (Gochang Black Raspberry Research Institute)
Publication Information
Korean Journal of Food Science and Technology / v.46, no.4, 2014 , pp. 489-497 More about this Journal
Abstract
We examined the effects of unripe black raspberry water extract (UBR-W) on lipid metabolism and oxidative stress in mice. C57BL/6J mice were divided into 4 groups: those administered a control diet (CTL), high-fat diet (HFD), UBR-W and simvastatin for 12 weeks. In the HFD group, LDL cholesterol were significantly higher than in the CTL group. However, the UBR-W treated group showed dose-dependent reduction of plasma LDL levels. Hepatic total lipid, TC, and malondialdehyde were significantly increased in hyperlipidemic mice. However, supplementation with either UBR-W or simvastatin effectively reduced these lipid profiles and lipid peroxidation. UBR-W increased mRNA expression of the LDL receptor, sterol regulatory element binding protein 2 (SREBP2), 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase and ATP-binding cassette transporter A1 (ABCA1) compared to that observed in the HFD group. In addition, UBR-W and simvastatin showed significantly reduced oxidized LDL uptake by the scavenger receptor CD36. These results suggest that UBR-W is useful for treatment and prevention of hyperlipidemia and lipid peroxidation.
Keywords
Unripe black raspberry; High-fat diet; Hyperlipidemia; Lipid metabolism; Oxidative stress;
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Times Cited By KSCI : 6  (Citation Analysis)
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1 Park SH, Kim JL, Lee ES, Han SY, Gong JH, Kang MK, Kang YH. Dietary ellagic acid attenuates oxidized LDL uptake and stimulates cholesterol efflux in murine macrophages1-3. J. Nutr. 141: 1931-1937 (2011)   DOI   ScienceOn
2 Goldstein JL, Brown MS. The LDL receptor. Arterioscler Thromb Vasc Biol. 29: 431-438 (2009)   DOI   ScienceOn
3 Weng YC, Chuang ST, Lin YC, Chuang CF, Chi TC, Chiu HL, Kuo YH, Su MJ. Caffeic acid phenylethyl amide protects against the metabolic consequences in diabetes mellitus induced by diet and streptozocin. Evid. Based Complement Alternat. Med. 984780 (2012)
4 Meddings JB, Spady DK, Dietschy JM. Kinetic characteristics and mechanisms of regulation of receptor-dependent and receptor-independent LDL transport in the liver of different animal species and humans. Am. Heart J. 113: 475-481 (1987)   DOI
5 Brown MS, Goldstein JL. A receptor-mediated pathway fir cholesterol homeostasis. Science. 232: 34-47 (1997)
6 Taniguchi T, Chen J and AD. Cooper Regulation of cholesterol 7 ${\alpha}$-hydroxylase gene expression in Hep-G2 cells. J. Biol. Chem. 269: 10071-10078 (1994)
7 Vijayakumar MV, Pandey V. Hypolipidemic effects of fenugreek seeds is mediated through inhibition of fat accumulation and upregulation of LDL receptor. Obesity (Silver Spring). 18: 667-674 (2010)   DOI
8 Tall AR, Wang N. Tangier disease as a test of the reverse cholesterol transport hypothesis. J. Clin. Invest. 106: 1205-1207 (2000)   DOI
9 Gallou. G, Ruelland A, Legras B, Maugendre D, Allanic H and Cloarec L. Plasma malondialdehyde in type 1 and type 2 diabetic patients. Clin. Chim. Acta. 214: 227-234 (1993)   DOI   ScienceOn
10 Yoon I, Cho JY, Kuk JH, Jang MY, Ahn TH and Park KH. Identification and activity of antioxidative compounds from Rubus coreanum fruit. Korean J. Food Sci. Technol. 34: 898-904 (2002)   과학기술학회마을
11 Seo JS, Choi YM, Lee SM, Kong SH, Lee JS. Antioxidant activities and antioxidant compounds of some specialty rices. .J Korean Soc. Food Sci. Nutr. 37: 129-135 (2008)   과학기술학회마을   DOI
12 Kissebah AH, Vydelingum N, Murray R, Evans DJ, Hartz AJ, Kalkhoff RK, Adams PW. Relation of body fat distribution to metabolic complications of obesity. J. Clin. Endocrinol. Metab. 54: 254-260 (1982)   DOI
13 Han J, Hajjar DP, Febbraio M, Nicholson AC. Native and modified low density lipoproteins increase the functional expression of the macrophage class B scavenger receptor, CD36. J. Biol. Chem. 272: 21654-21659 (1997)   DOI   ScienceOn
14 Kim JY, Nolte LA, Hansen PA, Han DH, Ferguson K, Thompson PA, Holloszy JO. High-fat diet-induced muscle insulin resistance: relationship to visceral fat mass. Am. J. Physiol, Regul. Integr. Comp. Physiol. 279: 2057-2065 (2000)
15 Krotkiewski M, Bjorntorp P, Sjostrom L, Smith U. Impact of obesity on metabolism in men and women. Importance of regional adipose tissue distribution. J. Clin. Invest. 72: 1150-1162 (1983)   DOI
16 Kim WJ, Kim SJ, Hwang MH, Jung HS, Kim HS and Cho HC. Effect of short term Rubus coreanus miquel supplementation on body composition maximum oxygen intake and blood lipid profiles, lactate and ammonia of middle age women with obesity during exercise program. J. Korean Soc. Living Environ. Sys. 15: 650-658 (2008)
17 Seeram NP, Adams LS, Zhang Y, Lee R, Sand D, Scheuller HS. Heber D. Blackberry, black raspberry, blueberry, cranberry, red raspberry, and strawberry extracts inhibit growth and stimulated apoptosis of human cancer cells in vitro. J. Agr. Food Chem. 54: 9329-9339 (2006)   DOI   ScienceOn
18 Jeong MY, Kim HL, Park JB, An HJ, Kim SH, Kim SJ, So HS, Park R, Um JY and Hong SH. Rubi Fructus (Rubus coreanus) inhibits differentiation to adipocytes in 3T3-L1 cells. Evidence-Based Complementary and Alternative Medicine 10 (2013)
19 Wang SY, Jiao H. Scavenging capacity of berry crops on superoxide radicals, hydrogen peroxide, hydroxyl radicals, and singlet oxygen. J. Agr. Food Chem. 48: 5677-5684 (2000)   DOI   ScienceOn
20 Ministry of Health and Welfare. The third Korea national health and nutrition examination survey; health examination. Ministry of Health and Welfare, Sejong, Korea. pp. 36-49 (2005)
21 Chen T, Hwang HJ, Rose ME, Nines RG, Stoner GD. Chemopreventive properties of black raspberries in N-nitrosomethylbenzylamine-induced rat esophageal tumorigenesis: Down regulation of cyclooxygenase-2, inducible nitric oxide synthase, and c-jun. Cancer Res. 66: 2853-2859 (2006)   DOI   ScienceOn
22 Jonasson L, Holm J, Skalli O, Bondjers G, Hansson GK. Regional accumulations of T cells, macrophages, and smooth muscle cells in the human atherosclerosis plaque. Arteriosclerosis. 6: 131-138 (1986)   DOI
23 Lee SJ, Lee MJ, Ko YJ, Choi HR, Jeong JT, Choi KM, Cha JD, Hwang SM, Jung HK, Park JH, Lee TB. Effects of extracts of unripe black raspberry and red ginseng on cholesterol synthesis. Korean J. Food Sci. Technol. 45: 628-635 (2013)   과학기술학회마을   DOI
24 Hsu HY, Nicholson AC, Hajjar DP. Inhibition of macrophage scavenger receptor activity by tumor necrosis factor-${\alpha}$ is transcriptionall and post trascriptionally regulated. J. Biol. Chem. 271: 7767-7773 (1996)   DOI
25 MolcnyiovA, StanckovA, JavorskM, Tkc I. Beneficial effect of simvastatin treatment on LDL oxidation and antioxidant protection is more pronounced in combined hyperlipidemia than in hypercholesterolemia. Pharmacol. Res. 54: 203-207 (2006)   DOI   ScienceOn
26 Yang HM, Oh SM, Lim SS, Shin HK, Oh YS, Kim JK. Antiinflammatory activities of Rubus coreanus depend on the degree of fruit ripening. Phytother. Res. 22: 102-107 (2011)
27 Choi HR, Lee SJ, Lee JH, Kwon JW, Lee HK, Jeong JT, Lee TB. Cholesterol-lowering effects of unripe black raspberry water extract. J. Koean Soc. Food Sci. Nutr. 42: 1899-1907 (2013)   과학기술학회마을   DOI
28 Song G1, Liu J, Zhao Z, Yu Y, Tian H, Yao S, Li G, Qin S. Simvastatin reduces atherogenesis and promotes the expression of hepatic genes associated with reverse cholesterol transport in apoE-knockout mice fed high-fat diet. Lipids in Health and Disease 10: 8 (2011)   DOI
29 Lei YF, Chen JL, Wei H, Xiong CM, Zhang YH, Ruan JL. Hypolipidemic and anti-inflammatory properties of Abacopterin A from Abacopteris penangiana in high-fat diet-induced hyperlipidemia mice. Food Chem. Toxicol. 49: 3206-3210 (2011)   DOI
30 Okawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbitruic acid reaction. Anal. Biochem. 95: 351-358 (1979)   DOI   ScienceOn
31 Ghasi S, Nwobodo E, Ofili Jo. Hypocholesterolemic effects of crude extract of leaf of Moringa oleifera Lam in high fat diet fed wistar rats. J. Ethnophamacol. 69: 21-25 (2000)   DOI   ScienceOn
32 Neuschwander-Tetri BA, Clark JM, Bass NM, Van Natta ML, Unalp-Arida A, Tonascia J, Zein CO, Brunt EM, Kleiner DE, McCullough AJ, Sanyal AJ, Diehl AM, Lavine JE, Chalasani N, Kowdley KV, NASH Clinical Research Network. Clinical, laboratory and histological associations in adults with non-alcoholic fatty liver disease. Hepatology. 52: 913-924 (2010).
33 Ferreira de Araujo PR, da Silva Santos V, Rodrigues Machado A, Gevehr Fernandes C, Silva JA, da Silva Rodrigues R. Benefits of blackberry nectar (Rubus spp.) relative to hypercholesterolemia and lipid peroxidation. Nutr. Hosp. 26: 984-990 (2011)
34 Kim SK, Kim CK, Lee KS, Kim JH, Hwang H, Jeoung D, Choe J, Wond MH, Lee H, Ha KS, Kwone YG, Kim YM. Aqueous extract of unripe Rubus coreanus fruit attenuates atherosclerosis by improving blood lipid profile and inhibiting NF-${\kappa}B$ acvication via phase II gene expression. J. Ethnopharmacol. 146: 515-524 (2013)   DOI   ScienceOn
35 Joo IW, Ryu JH, Oh HJ. The influence if Sam-Chil-Geun (Panax notoginseng) on the serum lipid levels and inflammations of rats with hyperlipidemia induced by poloxamer-407. Yonsei Med. Jul. 51: 504-510 (2010)   DOI   ScienceOn
36 Bhandary B, Lee HY, Back HI, Park SH, Kim MG, Kwon JW, Song JY, Lee HK, Kim HR, Chae SW, and Chae HJ. Immature Rubus coreanus shows a free radical-scavenging effect and inhibits cholesterol synthesis and secretion in liver cells. Indian J. Pharm. Sci. 74: 211-216 (2012)   DOI
37 Zhang X, Mao S, Luo G, Wei J, Berggren-Sderlund M, Nilsson-Ehle P, Xu N. Effects of simvastatin on apolipoprotein M in vivo and in vitro. Lipids Health Dis. 10: 112 (2011)   DOI
38 Kwon JW, Lee HK, Park HJ, Kwon TO, Choi HR, Song JY. Screening of biological activities to different ethanol extracts of Rubus coreanus miq. Korean J. Med. Sci. 19: 325-333 (2011)   과학기술학회마을   DOI
39 Lee SE, Sung JS, Jang IB, Kim GS, Ahn TJ, Han HS, Kim JE, Kim YO, Park CB, Cha SW, Ahn YS, Park HK, Bang JK, Seong NS. Investigation on antioxidant activity in plant resources. Korean J. Medicinal Crop Sci. 16: 356-370 (2008)   과학기술학회마을