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http://dx.doi.org/10.9721/KJFST.2014.46.3.334

Molecular Characterization and Toxin Profile of Bacillus cereus Strains Isolated from Ready-to-eat Foods  

Kim, Tae Sun (Health & Environment Institute of Gwangju)
Kim, Min Ji (Health & Environment Institute of Gwangju)
Kang, Yu Mi (Health & Environment Institute of Gwangju)
Oh, Geune (Health & Environment Institute of Gwangju)
Choi, Su Yeon (Health & Environment Institute of Gwangju)
Oh, Mu Sul (Health & Environment Institute of Gwangju)
Yang, Yong Shik (Health & Environment Institute of Gwangju)
Seo, Jung-Mi (Health & Environment Institute of Gwangju)
Ryu, Mi-Geum (Health & Environment Institute of Gwangju)
Kim, Eun-Sun (Health & Environment Institute of Gwangju)
Ha, Dong-Ryong (Health & Environment Institute of Gwangju)
Cho, Bae Sik (Health & Environment Institute of Gwangju)
Publication Information
Korean Journal of Food Science and Technology / v.46, no.3, 2014 , pp. 334-340 More about this Journal
Abstract
Toxin-producing Bacillus cereus is the causative agent of two different types of food poisoning: the emetic and the diarrheal types. This study was conducted to investigate the presence of enterotoxin and emetic toxin genes in 263 B. cereus isolated from 619 different ready-to-eat food items. Hemolytic enterotoxins hblA, hblC, and hblD were detected in 85.6, 41.1, and 76.8%, respectively, of the B. cereus isolates. About 67.0% (175/263) of the isolates presented all of three genes. Non-hemolytic enterotoxins nheA, nheB, and nheC were detected in 100, 97.0, and 68.4% of the isolates, respectively. Approximately 90.0% (236/263) of the isolates presented all of these three non-hemolytic enterotoxin genes. Emetic toxin gene, CER, was detected in 132 of 263 (50.2%) isolates. Computer-assisted cluster analysis of Rep-PCR profiles showed a high genetic diversity among the isolates. All B. cereus isolates from food samples tested in this study carried at least 6 of 10 toxin genes.
Keywords
B. cereus; enterotoxin; emetic toxin; PCR; genetic diversity;
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