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http://dx.doi.org/10.9721/KJFST.2013.45.1.84

Total Phenolics of Dried Platycodon grandiflorum and Its Effect on Growth of Human Cancer Cell Lines  

Hwang, Seong Yeon (Division of Marine Environment & Bioscience, Korea Maritime University)
Choi, Hyang Mi (Division of Marine Environment & Bioscience, Korea Maritime University)
Lim, Sun-Young (Division of Marine Environment & Bioscience, Korea Maritime University)
Publication Information
Korean Journal of Food Science and Technology / v.45, no.1, 2013 , pp. 84-89 More about this Journal
Abstract
We investigated the effects of extracts from dried Platycodon grandiflorum on total phenolic content and growth of cancer cell lines (HT-29 and AGS). Total phenolic contents of acetone/methylene chloride (A+M) extract and methanol (MeOH) fraction were 4.53 and 27.22 TAE mg/100 g, respectively. Among the fractions, n-butanol (n-BuOH) fraction contained the highest phenolic content. Treatments of crude extracts and fractions significantly inhibited the growth of HT-29 and AGS cancer cell lines (p<0.05). Among the fractions, n-BuOH fraction exhibited the highest inhibitory effect and was then sub-fractionated by reverse phase flash column chromatography (rfc). The rfc 1-3 exhibited a higher inhibitory effect on proliferation of both cancer cells. The rfc 1 contained the highest phenol content. Our results showed that n-BuOH fraction possessed a potent inhibitory effect on proliferation of human cancer cells. We suggested that this anticancer activity was partially related to the content of phenolic compounds.
Keywords
Platycodon grandiflorum; total phenol content; human cancer cell; growth inhibition; anticancer;
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Times Cited By KSCI : 4  (Citation Analysis)
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