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http://dx.doi.org/10.9721/KJFST.2011.43.2.224

Analysis of Zearalenone Contamination in Cereal-Based Products Using High Performance Liquid Chromatography-Fluorescence Detector and Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry  

Jang, Mi-Ran (Test & Analytical Center, Busan Regional KFDA)
Lee, Chang-Hee (Test & Analytical Center, Busan Regional KFDA)
Choi, In-Sun (Test & Analytical Center, Busan Regional KFDA)
Shin, Choon-Shik (Test & Analytical Center, Busan Regional KFDA)
Kim, Jin-Hee (Test & Analytical Center, Busan Regional KFDA)
Jang, Young-Mi (Test & Analytical Center, Busan Regional KFDA)
Kim, Dong-Sul (Test & Analytical Center, Busan Regional KFDA)
Ahn, Dong-Hyun (Department of Food Science and Technology, Pukyong National University)
Publication Information
Korean Journal of Food Science and Technology / v.43, no.2, 2011 , pp. 224-229 More about this Journal
Abstract
Zearalenone (ZEA) is an estrogenic mycotoxin mainly produced by Fusarium graminerum, a species which colonizes a wide variety of cereals, including wheat, barley and processed products. A survey of ZEA contamination was conducted on 141 dried confectioneries, 59 breads and rice cakes, 135 noodles and 101 other products, for a total of 432 commercial samples. Samples were analyzed by high performance liquid chromatography with fluorescence detection (HPLC-FLD) after immunoaffinity clean-up and was confirmed by liquid chromatography tandem mass spectrometry (LCMS/MS). The limits of detection and quantification were 2.0 and $6.0{\mu}g/kg$, respectively. The recovery ranged from 80.2% to 98.4% in the cereal based product. ZEA was detected in 38 samples (8.8% incidence), including 3 snack, 2 biscuit and 33 other cereal products. The ZEA contamination levels were in the range of $5.38-53.76{\mu}g/kg$. Finally, LC-MS/MS analysis of the contaminated samples was conducted to confirm the detected ZEA, and all 38 samples showing ZEA by HPLC-FLD were confirmed by LC-MS/MS.
Keywords
mycotoxin; zearalenone; immunoaffinity column; cereal;
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