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http://dx.doi.org/10.9721/KJFST.2011.43.2.213

Effect of Eupatorium japonicum Extract on the Metastasis, Invasion and Adhesion of MDA-MB-231 Human Breast Cancer Cells  

Woo, Eun-Young (Department of Food Science and Nutrition, Hallym University)
Park, So-Young (Department of Food Science and Nutrition, Hallym University)
Kwon, Soo-Jin (Department of Food Science and Nutrition, Hallym University)
Kwon, Gyoo-Taik (Department of Food Science and Nutrition, Hallym University)
Kim, Jong-Dae (Department of Food Science and Biotechnology, Kangwon National University)
Lim, Soon-Sung (Department of Food Science and Nutrition, Hallym University)
Yoon, Jung-Han (Department of Food Science and Nutrition, Hallym University)
Publication Information
Korean Journal of Food Science and Technology / v.43, no.2, 2011 , pp. 213-219 More about this Journal
Abstract
The metastatic effect of Eupatorium japonicum extract (EJE) on MDA-MB-231 human breast cancer cells was investigated. MDA-MB-231 cells were treated with various concentrations of EJE (0, 5, 10 and $20{\mu}g/mL$). EJE inhibited cell migration, invasion and adhesion of MDA-MB-231 cells in dose-dependent manners. Gelatin zymography exhibited that EJE significantly down regulated secretion of matrix metalloproteinase (MMP)-9 and MMP-2. EJE decreased the protein levels of tissue inhibitor of metalloproteinase (TIMP)-1 but increased TIMP-2 levels. Additionally, EJE reduced the protein and mRNA levels of urokinase-type plasminogen activator (uPA), vascular endothelial growth factor (VEGF) and intercellular adhesion molecule (ICAM). In several solvent fractions of EJE, the hexane fraction markedly decreased MDAMB-231 cell migration. Thus, these finding suggest that EJE may be a potential antimetastatic agent, which can considerably inhibit the metastatic and invasive capacity of breast cancer cells.
Keywords
Eupatorium japonicum; breast cancer; metastasis; matrix metalloproteinase; urokinase-type plasminogen activator;
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Times Cited By KSCI : 3  (Citation Analysis)
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