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The Rapid Detection of Antioxidants from Safflower Seeds (Carthamus tinctorius L.) Using Hyphenated-HPLC Techniques  

Kim, Su-Jin (Korea Institute of Science and Technology (KIST) Gangneung Institute)
Kim, Sang-Min (Korea Institute of Science and Technology (KIST) Gangneung Institute)
Kang, Suk-Woo (Korea Institute of Science and Technology (KIST) Gangneung Institute)
Um, Byung-Hun (Korea Institute of Science and Technology (KIST) Gangneung Institute)
Publication Information
Korean Journal of Food Science and Technology / v.42, no.4, 2010 , pp. 414-419 More about this Journal
Abstract
Hyphenated-HPLC techniques combine the separation power of HPLC with the structural and bioactivity information provided by NMR, ESI/MS, and an on-line antioxidant screening system. The major advantages over the traditional off-line techniques are rapidity and efficiency. In this study, we used hyphenated HPLC techniques including online HPLC-ABTS, LC-NMR, and LC-MS todirectly identify the major antioxidants of safflower (Carthamus tinctorius L.) seeds. The results demonstrated that the major antioxidant compounds from on-line HPLC-ABTS analysis were identified as 8'-hydroxyarcgenin-4'-O-$\beta$-D-glucoside, N-(p-coumaroyl) serotonin, and N-feruloylserotonin. Among them, N-feruloylserotonin accounted for almost 50% of the ABTS radical scavenging activity of the total extract. The results demonstrate that HPLC hyphenated techniques can be used to rapidly screen and structurally identify antioxidants from crude plant extracts.
Keywords
antioxidants; Carthamus tinctorius L.; LC-MS; LC-NMR; on-line HPLC-ABTS system;
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