Browse > Article

Immunomodulating Activity of Laminaria japonica Polysaccharides  

Ryu, Deok-Seon (Department of Biomedical Laboratory Science, Inje University)
Oh, Seung-Min (Department of Biomedical Laboratory Science, Inje University)
Kim, Ki-Hoon (Department of Biomedical Laboratory Science, Inje University)
Kim, Soo-Hwan (Department of Biomedical Laboratory Science, Inje University)
Choi, Hyun-Ju (Department of Biomedical Laboratory Science, Inje University)
Lee, Dong-Seok (Department of Biomedical Laboratory Science, Inje University)
Publication Information
Korean Journal of Food Science and Technology / v.42, no.3, 2010 , pp. 350-354 More about this Journal
Abstract
Laminaria japonica polysaccharides (LP) were prepared from L. japonica through hot water extraction, ultrafiltration and gel chromatography. In this study, we investigated the immunomodulating activity of LP (0.25-1 mg/mL) on the mitogen/alloantigen reactive proliferation and killing activity of the Balb/c mouse splenocytes. The LP directly induced the proliferation of splenocytes that was stimulated with mitogen or alloantigen in a dose-dependent manner. The killing activity of cytotoxic T lymphocytes (CTLs) and lymphokine activated killer cells (LAKs) were enhanced significantly in the LP treated cells. Also, the treatment of splenocytes with LP increased production of interleukin-2 (IL-2). These results suggest that polysaccharides from L. japonica show a substantial immunomodulating activity in mouse immune cells.
Keywords
Laminaria japonica; polysaccharides; immunomodulating activity;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Nikonenko BV, Apt AS, Mezhlumova MB, Avdienko VG, Yeremeev W, Moroz AM. Influence of the mouse Bcq, Tbc-1, and Xid genes on resistance and immune responses to tuberculosis infection and efficacy of Bacille Colmette-Guerin (BCG) vaccination. Clin. Exp. Immunol. 104: 37-43 (1996)   DOI
2 Hwang YC, Shin KS. Characterization of immuno-stimulating polysaccharides isolated from Korean persimmon vinegar. Korean J. Food Sci. Technol. 40: 220-227 (2008)   과학기술학회마을
3 Vetvicka V, Yvin JC. Effects of marine $\beta$-1,3 glucan on immune reactions. Int. J. Immunopharmaco. 4: 721-730 (2004)   DOI   ScienceOn
4 Yagoob P, Carder PC. The effect of fatty acids on lymphocyte functions. Int. J. Biochem. 12: 1705-1709 (1993)
5 Calder PC, Bond JA, Bevan SJ, Newsholeme EA. Unsaturated fatty acids inhibit interleukin-2 production by concanavalin Astimulated lymphocytes. Proc. Nutr. Soc. 50: 171-175 (1991)
6 Cyster JG. Chemokines and cell migration in secondary lymphoid organs. Science 286: 2098-2102 (1999)   DOI
7 Lee IA, Popov AM, Sanina NM, Kostetsky EY, Novikova OD, Reunov AV, Nagorskaya VP, Shnyrov VL. Morphological and immunological characterization of immunostimulatory complexes based on glycoglycerolipids from Laminaria japonica. Biochim. Biophys. Acta 51: 263-272 (2004)
8 Ha JC, Kim YH, Woo WH, Nam SY. Promotion of nonspecific cytotoxic T lymphocyte activity by bo-yang-hwan-oh-tang. Korean J. Pharmacogn. 32: 226-232 (2001)
9 Diefenbach A, Rauler DH. The innate immune response to tumors and its role in the induction of T-cell immunity. Immunol. Rev. 188: 9-21 (2002)   DOI   ScienceOn
10 Cho SH, Yang KM, Bae BS, Im SA, Yu RN. Effect of sea tangel intake on cytokine production in macrophage from normal and diabetic mice. J. Korean Soc. Food Sci. Nutr. 27: 952-959 (1998)
11 Shida K, Suzuki T, Kiyoshima SJ, Shimada S, Nanno M. Essential roles of monocytes in stimulating human peripheral blood mononuclear cells with Lactobacillus casei to produce cytokines and augment natural killer cell activity. Clin. Vaccine Immunol. 13: 997-1003 (2006)   DOI   ScienceOn
12 Agarwal BB, Traquna PR, Eessalu TE. Modulation of receptor and cytotoxic response of tumor necrosis factor-L by various lectins. J. Biol. Chem. 261: 13652-13656 (1986)
13 Elyakova LA, Zvyagintseva TN. A study of the laminarins of some Far-Eastern, brown seaweeds. Carbohyd. Res. 34: 241-248 (1974)   DOI   ScienceOn
14 Oh CK, Oh MC, Kim SH, Rhim SB, Kim SH. Antimutagenic and antimicrobial effect of ethanol extracts from sea-mustard and sea tangle. J. Korean Fish. Soc. 31: 90-94 (1998)
15 Lahaye M. Marine algae as sources of fibers contents in some sea vegetable. J. Sci. Food Agr. 54: 587-594 (1991)   DOI
16 Kim YY, Lee KW, Kim GB, Cho YJ. Studies on physicochemical and biological properties of depolymerized alginate from sea tangle, Laminaria japonicus by heating hydrolysis. J. Korean Fish. Soc. 33: 393-398 (2000)
17 Ryu BH, Kim DS, Cho KJ, Sin DB. Antitumor activity of seaweeds toward sarcoma-180. Korean J. Food Sci. Technol. 21: 595-600 (1989)
18 Nakashima H, Kido N, Kobayashi N, Motoki M, Neushul M, Yamamoto N. Purification and characterization of an avian myeloblastosis and human immunodeficiency virus reverse transcriptase inhibitor sulfated polysaccharide extracted from sea algae. Antimicrob. Agents Ch. 31: 1524-1528 (1987)   DOI   ScienceOn
19 Kim KH, Kim YW, Kim HB, Lee BJ, Lee DS. Anti-apoptotic activity of laminarin polysaccharides and their enzymatically hydrolyzed oligosaccharides from Laminaria japonica. Biotechnol. Lett. 28: 439-446 (2006)   DOI   ScienceOn
20 Han SB, Kim YH, Lee CW, Park SM, Lee HY, Ahn KS, Kim IH, Kim HM. Characteristic immunostimulation by angelan isolated from Angelica gigas Nakai. Immunopharmacology 40: 39-48 (1998)   DOI   ScienceOn
21 Amirghofran Z, Azadbakht M, Karimi MH. Evaluation of the immunomodulatory effect of five berbal plants. J. Ethnopharmacol. 72: 167-172 (2000)   DOI   ScienceOn
22 Maeda YY, Watanabe ST, Chihara G, Rokutanda M. T-cell mediated vascular dilatation and hemorrhage induced by antitumour polysaccharides. Int. J. Immunopharmaco. 6: 493-501 (1984)   DOI   ScienceOn
23 Sugawara I, Lee KC, Wong M. Schizophyllan(SPG)-treated macrophages and antitumor activities against syngeneic and allogeneic tumor cells. Cancer Immunol. Immun. 16: 137-144 (1984)
24 Akiyama J, Kawamura T, Gotohda E, Yamada Y, Hosokawa M, Kodama T, Kobayashi H. Immunochemotheraphy of transplanted KMT-17 tumor in WKA rats by combination of cyclophosphamide and immunostimulatory protein-bound polysaccharide isolated from basidiomycetes. Cancer Res. 37: 3042-3045 (1977)
25 Oldham RK. Biological response modifiers. J. Natl. Cancer Inst. 70: 789-796 (1983)
26 Yun YS, Lee YS, Jo SK, Jung IS. Inhibition of autochthonous tumor by ethanol insoluble fraction from Panax ginseng as an immunomodulator. Planta. Med. 59: 521-524 (1993)   DOI
27 Fahey TJ, Myers WP. Documented hyperparathyroidism of thirtysix years' duration. Cancer 35: 803-807 (1975)   DOI   ScienceOn
28 Oettgen HF. Biological agents in cancer therapy; cytokines, monoclonal antibodies, and vaccines. J. Cancer Res. Clin. 116: 116-119 (1990)   DOI
29 Bergelson LD. Serum gangliosides as endogenous immunomodulators. Immunol. Today 16: 483-486 (1995)   DOI   ScienceOn