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Effect of Electrolyzed Water and Citric acid On Quality Enhancement and Microbial Inhibition in Head Lettuce  

Jin, Yong-Guo (College of Food Science and Technology, Huazhong Agricultural University)
Kim, Tae-Woong (Division of Food and Biotechnology, Kangwon National University)
Ding, Tian (Division of Food and Biotechnology, Kangwon National University)
Oh, Deog-Hwan (Division of Food and Biotechnology, Kangwon National University)
Publication Information
Korean Journal of Food Science and Technology / v.41, no.5, 2009 , pp. 578-586 More about this Journal
Abstract
This study was conducted to determine the effects of alkaline electrolyzed water (AIEW), acidic electrolyzed water (AcEW), 1% citric acid, and 100 ppm sodium hypochlorite, either alone or in combination with citric acid, in reducing the populations of spoilage bacteria and foodborne pathogens (Listeria monocytogenes and Escherichia coli O157:H7) on lettuce at various exposure times (3, 5, and 10 min) with different dipping temperatures (1, 20, 40, and $50^{\circ}C$). In addition, the inhibitory effect of alkaline electrolyzed water combined with citric acid on the browning reaction during storage at $4^{\circ}C$ for 15 days was investigated. Compared to the untreated control, electrolyzed water more effectively reduced the number of total bacteria, mold, and yeast than 100 ppm sodium hypochlorite under the same treatment conditions. All treatments exposed for 5 min significantly reduced the numbers of total bacteria, yeast, and mold on head lettuce. The inactivation effect of each treatment on head lettuce was enhanced as the dipping temperature increased from 1 to $50^{\circ}C$, but there was no significantly difference at temperatures greater than $40^{\circ}C$ (p<0.05). The total counts of yeast and mold in head lettuce were completely eliminated when a combination of 1% citric acid and AlEW treatment was used at temperatures greater than $40^{\circ}C$. However, decreased reduction in L. monocytogenes (2.81 log CFU/g), and E. coli O157:H7 (2.93 log CFU/g) on head lettuce was observed under these treatment conditions. In addition, enhanced anti-browning effect was observed when the samples were subjected to both 1% citric acid and AlEW treatment at temperatures greater than $40^{\circ}C$ compared to when single treatments alone were used. Thus, this combined treatment might be considered a potentially beneficial sanitizing method for improving the quality and safety of head lettuce.
Keywords
electrolyzed water; inactivation; head lettuce; dipping temperature; dipping time; anti-browning effect;
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Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By SCOPUS : 4
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1 Ana A, Francisco AT, Mara IG. Minimal processing for healthy traditional foods. Trends Food Sci. Tech. 17: 513-519 (2006)   DOI   ScienceOn
2 Daniel L, Yanyun Z. Innovations in the development and application of edible coatings for fresh and minimally processed fruits and vegetables. Compr. Rev. Food Sci. F. 6: 60-75 (2007)   DOI   ScienceOn
3 Ahvenainen R. New approaches in improving the shelf life of minimally processed fruit and vegetables. Trends Food Sci. Tech. 7: 179-186 (1996)   DOI   ScienceOn
4 Jongen, W. Introduction. pp. 1-4. In: Fruit and Vegetable Processing. W. Jongen (ed). Wood-head Publishing Lid. and CRC Press LLC, Inc. Boca Raton, FL, USA (2002)
5 Salunkhe DK, Bolin HR, Reddy NR. Minimal Processing Storage, Processing, and Nutritional Quality of Fruit and Vegetables. Vol. 2, pp. 128-153. Processed Fruit and Vegetables. Salunkhe DK (ed). CRC Press, Boca Raton, FL, USA (1991)
6 Shigenobu K, Seiichiro I. Microbial control of fresh produce using electrolyzed water. Jpn. Agr. Res. 41: 273-282 (2007)
7 Jung CY, Choi LG. SPSSWIN for Statistics Analysis, Version 10.0, 4th ed. Muyok Publishing Co., Seoul, Korea. pp. 276-283 (2002)
8 Vandekinderen I, Van Camp J, Devlieghere F, Ragaert P, Veramme K, Bernaert N, Denon Q, de Meulenaer B. Evaluation of the use of decontamination agents during fresh-cut leek processingand quantification of their effect on its total quality by means of a multidisciplinary approach. Innov. Food Sci. Emerg. Tech. 10: 363-373 (2009)   DOI   ScienceOn
9 Lee SH, Jang MS. Effects of electrolyzed water and chlorinated water on sensory and microbiological characteristics of lettuce. Korean J. Soc. Food Cook. Sci. 20: 45-53 (2004)
10 Park BK, Oh MH, Oh DH. Effect of electrolyzed and organic acids on the growth inhibition of Listeria monocytogenes on lettuce. Korean J. Food Preserv. 11: 530-537 (2004)   과학기술학회마을
11 Singh N, Singh RK, Bhunia AK, Stroshine RL. Efficacy of chlorine dioxide, ozone, and thyme essential oil or sequential washing in killing Escherichia coli O157:H7 on lettuce and baby carrots. Lebensm.-Wiss. Technol. 35: 720-729 (2002)   DOI   ScienceOn
12 Park WP, Cho SH, Lee DS. Screening of antibrowning agents for minimally processed vegetables. Korean J. Food Sci. Technol. 30: 278-282 (1998)   ScienceOn
13 Cha HS, Kim SI, Kim BS, Kim SH, Park SJ, Cho HS, Choi HY. Effect of inhibition on browning and microbial growth of minimally processed lettuce. Korean J. Food Preserv. 11: 331-335 (2004)   과학기술학회마을
14 Shigenobu K, Kazuhiro F, Kazuhiko I. Decontamination effect of frozen acidic electrolyzed water on lettuce. J. Food Protect. 65: 411-414 (2002)   PUBMED   ScienceOn
15 Lee SH, Jo HS, Kim SH. Effect of organic acids on growth and heat resistance of Listeria monocytogenes scott A. J. Korean Soc. Food Nutr. 23: 293-297 (1994)   ScienceOn
16 Peter R, Wim V, Frank D, Johan D. Consumer perception and choice of minimally processed vegetables and packaged fruits. Food Qual. Prefer. 15: 259-270 (2004)   DOI   ScienceOn
17 Shewfelt RL. Quality of fruit and vegetables. Food Technol.-Chicago 44: 99-106 (1990)
18 Torriani S. Massa S. Bacteriological survey on ready-to-use sliced carrots. Lebensm.-Wiss. Technol. 27, 487-490 (1994)   DOI   ScienceOn
19 Kim C, Hung YC, Brackett RE. Efficacy of electrolyzed oxidizing (EO) and chemically modified water on different types of foodborne pathogens. Int. J. Food Microbiol. 61: 199-207 (2002)   DOI   ScienceOn
20 Youm HJ, Ko JK, Kim MR, Cho YS, Chun HK, Song KB. Effect of aqueous chlorine dioxide and citric acid treatment on microbial safety and quality control of minimally processed and refrigerated (MPR) salad. Korea J. Food Sci. Technol. 37: 129-133 (2005)   ScienceOn
21 Hurst JK, Barrette WC, Michel Jr BR, Rosen H. Hypochlorous acid and myeloperoxidase-catalyzed oxidation of ironsulfur clusters in bacterial respiratory dehydrogenase. Eur. J. Biochem. 202: 1275-1282 (1991)   DOI   ScienceOn
22 Koukichi H. Physico-chemical properties of electrolyzed functional water and its application. Fragr. J. 3: 18-22 (1999)
23 Gardial SF, Clemons DS, Woodruff RB, Schumann DW, Burns MJ. Comparing consumer's recall of prepurchase and postpurchase product evaluation experiences and postpurchase product evaluation experiences. J. Consum. Res. 20: 548-560 (1994)   DOI   ScienceOn
24 Kim YJ, Cho JI, Kim KS. Evaluation of washing treatment using chlorine and heat hurdles on natural microflora in fresh lettuces collected from domestic markets. Food Eng. Progress 6: 329-335 (2002)
25 Carlin F, Nguyen-the C, Silva AAD. Factors affecting the growth of Listeria monocytogenes on minimally processed fresh endive. J. Appl. Bacteriol. 78: 636-646 (1995)   ScienceOn
26 Maribel A, Josep U, Mrcia O, Isabel A, Inmaculada V. Efficacy of neutral electrolyzed water (NEW) for redicing microbial contamination on minimally-processed vegetables. Int. J. Food Microbiol. 123: 151-158 (2008)   DOI   ScienceOn
27 Wiley RC. Minimally Processed Refrigerated Fruits and Vegetables, Chapman & Hall, Inc., New York, NY, USA. pp. 1-14 (1994)
28 Shigenobu K, Kyoichiro Y, Yoshinori K, Seiichiro I, Kazuhiko I. Effect of mild heat pre-treatment with alkaline electrolyzed water on the efficacy of acidic electrolyzed water against Escherichia coli O157:H7 and Salmonella on lettuce. Food Microbiol. 21:559-566 (2004)   DOI   ScienceOn
29 Boyette MD, Ritchie DF, Carbollo SJ, Blankenship SM, Sanders DC. Chorination and postharvest disease control. Hort. Technol. 3: 395-400 (1993)
30 Nascimento MS, Silva N, Catanozi MPLM, Silva KC. Effects of different disinfection treatments on the natural microbiota of lettuce. J. Food Protect. 66: 1697-1700 (2003)   PUBMED   ScienceOn
31 Delaquis PJ, Stewart S, Toivonen PMA, Moyls AL. Effect of warm, chlorinated water on the microbial flora of shredded iceberg lettuce. Food Res. Int. 32: 7-14 (1999)   DOI   ScienceOn
32 Kennedy E, Meyers L, Layden W. The 1995 Dietary Guidelines for America: An Overview. J. Am. Diet. Assoc. 96: 234-237 (1996)   DOI   ScienceOn
33 Beuchat LR, Nail BV, Adler BB, Clavero MRS. Efficacy of spray application of chlorinated water in killing pathogenic bacteria on raw apples, tomatoes, and lettuce. J. Food Protect. 61: 1305-1311 (1998)   PUBMED   ScienceOn
34 Marshall JJ, Duxbury L, Heslop LA. Coping with household stress in the 1990s: Who uses convenience foods and do they help? Adv. Consum. Res. 22: 729-734 (1995)   ScienceOn
35 Beuchat LR, Brackett RE. Survival and growth of Listeria monocytogenes on lettuce as influenced by shredding, chlorine treatment, modified, atmosphere packaging and temperature. J. Food Sci. 55: 755-759 (1992)   DOI
36 Li Y, Brackett RE, Shewfelt RL, Beuchat LR. Changes in appearance and natural microflora on iceberg lettuce treated in warm, chlorinated water and then stored at refrigeration temperature. Food Microbiol. 18: 299-308 (2001)   DOI   ScienceOn