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Acute Oral Toxicity and Anti-obesity Effect of Diglyceride Preparation Containing Conjugated Linoleic Acid in Rat  

Hong, Soon-Gi (KT&G Central Research Institute)
Park, Chae-Kyu (KT&G Central Research Institute)
Lee, Mi-Ja (KT&G Central Research Institute)
Chung, Shin-Gyo (College of Agriculture & Life Sciences, KyungPook National University)
Lee, Young-Ho (Ilshinwells Co., Ltd.)
Hyun, Sun-Hee (KT&G Central Research Institute)
Publication Information
Korean Journal of Food Science and Technology / v.41, no.3, 2009 , pp. 320-325 More about this Journal
Abstract
The present study was carried out to investigate the acute oral toxicity and anti-obesity effects of a diglyceride preparation containing conjugated linoleic acid (DG+CLA). To test its acute oral toxicity, the DG+CLA was injected into 30 rats (15 males and 15 females) at dosage of 2,000 mg/kg and 5,000 mg/kg. Mortality rates, clinical signs, and body weight changes were monitored for 14 days following administration. According to the results, the lethal dose ($LD_50$) of DG+CLA was determined as >5,000 mg/kg in both sexes. There were no significant changes in general conditions, clinical signs, body weight, and gross lesions between the vehicle control and DG+CLA groups. For the anti-obesity studies, obese Zucker rats were randomly divided into 4 groups and fed saline, soybean oil, diglyceride, and DG+CLA, respectively, for 8 weeks. The DG+CLA groups presented significant differences in body weight, food efficiency ratio, serum lipid levels, and fat weight. Overall, the results showed that the DG+CLA did not have acute oral toxicity and reduced body weight, serum lipid levels, and fat gain.
Keywords
diglyceride preparation containing conjugated linoleic acid; acute oral toxicity; anti-obesity; serum lipid levels; fat weight;
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