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Evaluation of Antimicrobial Activity and Mutagenicity of Ozonized Olive Oil  

Jang, Il-Woong (Department of Food Biotechnology, Woosuk University)
Lee, Seung-Jae (Department of Food Biotechnology, Woosuk University)
Ahn, Jeung-Youb (R&D Institute, Saenggreen Co.)
Miura, Toshiaki (Graduate School of Pharmaceutical Science, Hokkaido University)
Jung, Mun-Yhung (Department of Food Industry & Management, Woosuk University)
Choi, Dong-Seong (Department of Food Biotechnology, Woosuk University)
Publication Information
Korean Journal of Food Science and Technology / v.38, no.6, 2006 , pp. 805-809 More about this Journal
Abstract
Ozonized olive oil was tested for its mutagenic potential in a Salmonella/microsome assay. Additionally, antimicrobial activity was tested against Propionibacterium acnes, Staphylococcus epidermidis, Staphylococcus aureus and Pseudomonas aeruginosa, pathogenic strains related to acne, using the paper disk and agar dilution method. Ozonized olive oil showed antimicrobial activities against all the strains tested, with minimal inhibitory concentrations (MICs) values in a range of 2${\sim}$10 mg/mL. Mutagenicity of ozonized olive oil was evaluated with Salmonells typhimurium TA98, TA100 and Ta1535, with and without addition of S9 mixture. No increase in the number of $his^{+}$ revertants over the negative control (solvent and non-ozonized olive oil) values was observed with TA98 (1,000 ${\mu}g/plate$), TA100 (1,500 ${\mu}g/plate$) and TA1535 (1,500 ${\mu}g/plate$) strains. The results from this study suggested that ozonized olive oil does not show any mutagenic potential.
Keywords
ozonized olive oil; antimicrobial activity; acne; mutagenicity; Ames test;
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