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Rapid Quantitative Analysis of Isoflavones using TLC  

Kim, Kyung-Seon (Department of Food Science and Biotechnology, KyungHee University)
Park, Kwan-Hwa (Department of Food Science and Technology, Seoul National University)
Baik, Moo-Yeol (Department of Food Science and Biotechnology, KyungHee University)
Kang, Kil-Jin (Containers and Packaging Division, Korea Food and Drug Administration)
Park, Cheon-Seok (Department of Food Science and Biotechnology, KyungHee University)
Publication Information
Korean Journal of Food Science and Technology / v.36, no.4, 2004 , pp. 558-562 More about this Journal
Abstract
Conditions for rapid quantification of isoflavones were studied. Rapid and clear separation of isoflavones (genistin and daidzin) was obtained using solvent system of chloroform : methanol : water : acetic acid (60 : 30 : 10 : 0.5, v/v/v/v). Quantification of each isoflavone separated by TLC was conducted by densitometry analysis. Genistin and daidzin were quantified in $0.15-1.80\;{\mu}g/{\mu}L$ range with 99% confidence. Concentrations of isoflavones in soybeans and kudzu roots originated from Korea were determined, and validity of TLC method for quantification of isoflavones was confirmed by comparison with HPLC analysis.
Keywords
thin layer chromatography; isoflavone; high performance liquid chromatography; genistin; daidzin;
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Times Cited By KSCI : 1  (Citation Analysis)
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