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http://dx.doi.org/10.5352/JLS.2015.25.7.801

Extract from Prunus mume Sieb. et Zucc. Fruit Prevents LPS-induced Homotypic Aggregation of Monocytic THP-1 Cells via Suppression of Nitric Oxide Production and NF-κB Activation  

Lee, Hye-Rim (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, Dankook University)
Park, Youngsook (Department of Life Systems, Sookmyung Women’s University)
Kim, Hyun Jeong (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, Dankook University)
Lee, Aram (Department of Life Systems, Sookmyung Women’s University)
Choi, Jihea (Department of Life Systems, Sookmyung Women’s University)
Pyee, Jaeho (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, Dankook University)
Park, Heonyong (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, Dankook University)
Kim, Jongmin (Department of Life Systems, Sookmyung Women’s University)
Publication Information
Journal of Life Science / v.25, no.7, 2015 , pp. 801-809 More about this Journal
Abstract
Homotypic cell adhesion (homotypic aggregation) in activated monocytes plays a central role in physiological and pathological processes including inflammatory responses, differentiation and migration. The extract of the Prunus mume Sieb. et Zucc. fruit (Maesil) has potential benefits to human health; such as anti-viral, anti-microbial, and anti-cancer activities. Indeed, Maesil extract may modulate inflammatory responses via interference with homotypic aggregation in monocytes. In the present study, the molecular mechanisms underpinning the therapeutic efficacy of Maesil extract in inflammatory diseases were investigated. It was found that Maesil extract inhibited homotypic aggregation in lipopolysaccharide (LPS)-activated monocytes. This was mediated by reduction of nitric oxide (NO) production, partly via inhibition of inducible nitric oxide synthase (iNOS) expression in LPS-activated THP-1 cells. It was confirmed that NO inhibition is a key mechanism in Maesil induced blockade of monocyte aggregation through identification of reversal of this inhibitory effect by the NO-producing agent S-nitroso-N-acetyl penicillamine (SNAP). In addition, Maesil extract significantly attenuated LPS-induced IκB-α phosphorylation and NF-κB translocation into the nucleus. In conclusion, Maesil extract exerts anti-inflammatory effects via inhibition of homotypic aggregation of LPS-activated monocytes through mechanisms involving the suppression of NO production and NF-κB activity, suggesting Maesil extract as a potential therapeutic candidate for the prevention and treatment of chronic inflammatory diseases.
Keywords
Homotypic aggregation; inflammationnitric oxide; maesil extract; NF-κ B;
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