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http://dx.doi.org/10.5352/JLS.2015.25.7.795

Optimization of Hydrolysis Using Oyster and Oyster Cooking Drip  

Ryu, Tae-hyun (Department of Food Science & Technology, Pukyoung National University)
Kim, Jin-hee (Department of Food Science & Technology, Pukyoung National University)
Shin, Jiyoung (Department of Food Science & Technology, Pukyoung National University)
Kim, Sung-hoon (Department of Food Science & Technology, Pukyoung National University)
Yang, Ji-young (Department of Food Science & Technology, Pukyoung National University)
Publication Information
Journal of Life Science / v.25, no.7, 2015 , pp. 795-800 More about this Journal
Abstract
Oyster is a nutritionally good food ingredient. Also, oyster is used to make source for taste and flavor. This study tried to investigate optimal condition of hydrolysis of oyster and oyster cooking drip for better amino acid content to make good taste and flavor. And then this study characterized hydrolysate of oyster and oyster cooking drip. Enzymes are Acalase, Flavourzyme, Neutrase, and Protamax. The optimal condition for the highest enzyme activity is given by the company. Under the best condition of each enzymes, they react with the homogenized oyster and oyster cooking drip for 0.5, 1.0, 1.5, 2, 4, 6 hr. The degree of oysters’ hydrolysis is 13.2±0.1%. But, in the case of using enzyme, the rate of hydrolysis sharply increased as time went on during 2 hr. After 8 hr, the rate is 36.9~40.5%. Protamax showed 27.4±0.4% of hydrolysis rate in 2 hr. And the degree of oyster cooking drop hydrolysis is 42.7±0.1%. The highest of hydrolysate concentration is 72.1±0.1% using protamax. In the case of oyster, it has a similar tendency of all enzymes. Otherwise, the hydrolysate of oyster cooking drip had a difference among the enzymes. Composition of free amino acid of hydrolysate using protamax was investigated how much time showed highest rate of hydrolysis to find best amino acid composition. Hydrolysis using Protamax during 6 hr is selected for best condition.
Keywords
Amino acids; enzymes; hydrolysate; oyster; oyster cooking drip;
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