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http://dx.doi.org/10.5352/JLS.2015.25.7.773

Anti-adipogenic, Anti-inflammatory, and Anti-proliferative Activities of Extracts from Lees and Nuruk  

Son, Jung-Bin (Department of Biological Sciences, Andong National University)
Lee, Seung Hoon (Department of Biological Sciences, Andong National University)
Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University)
Shin, Woo-Chang (Research Institute, Kooksoondang Brewery Co. Ltd.)
Kim, Jong-Sik (Department of Biological Sciences, Andong National University)
Publication Information
Journal of Life Science / v.25, no.7, 2015 , pp. 773-779 More about this Journal
Abstract
This study examined extracts from five different kinds of lees and nuruk and their organic solvent fractions in terms of several biological functions, such as anti-adipogenic, anti-inflammatory, and anti-proliferative activities. The anti-adipogenic activity was investigated by treating mouse pre-adipocyte 3T3-L1 cells with one extract (YE) and four organic solvent fractions (YAc, PAc, RAc, and WPAc) during adipogenesis. Among the treated samples, the ethyl acetate fraction of W-Ju lees (WPAc) showed the strongest anti-adipogenic effect, which was confirmed with oil red O staining and down-regulation of pro-adipogenic genes such as PPAR-gamma and SCD-1. Treatment with WPAc also reduced the expression of PPAR-gamma in a time-dependent manner. The effects of five different extracts were examined on nitric oxide (NO) production in mouse RAW 264.7 cells to determine anti-inflammatory activity. The ethyl acetate fraction of B-Ju lees (PAc) significantly decreased NO production in LPS-stimulated RAW 264.7 cells and it also inhibited NO production in a dose-dependent manner. The PAc fraction also dramatically decreased the viability of human colorectal cancer HCT116 cells in a dose-dependent manner. In addition, PAc increased the expression of NAG-1 and ATF3 genes in a dose dependent manner. Overall, these results indicate that lees and nuruk have several biological functions, including anti-adipogenic, anti-inflammatory, and anti-proliferative activities.
Keywords
Anti-adipogenesis; anti-inflammation; anti-proliferation; lees; nuruk;
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