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http://dx.doi.org/10.7856/kjcls.2017.28.1.131

Genotoxicological Safety Evaluation of Crude Antifungal Compounds Produced by Bacillus subtilis SN7  

Chang, Hae-Choon (Dept. of Food and Nutrition, Chosun University)
Koh, Sang-Bum (KOREA Testing & Research Health Care Research Laboratory, Institute)
Lee, Jae-Joon (Dept. of Food and Nutrition, Chosun University)
Publication Information
The Korean Journal of Community Living Science / v.28, no.1, 2017 , pp. 131-141 More about this Journal
Abstract
This study was carried out to perform genotoxicological safety evaluation of crude antifungal compounds produced by Bacillus subtilis SN7 (B. subtilis SN7) isolated from meju. Bacterial reverse mutation assay with Salmonella typhimurium TA98, TA100, TA1535, and TA1537 or Escherichia coli WP2uvrA in the presence and absence of the S9 metabolic activation system was carried out, and the crude antifungal compounds produced by B. subtilis SN7 showed no significant increase in the number of revertant colonies. In the chromosomal aberration tests using Chinese hamster lung (CHL) cells, sample treatment groups showed no increase in the frequency of chromosome aberrations compared to the negative control group. Furthermore, in the micronucleus formation test, the crude antifungal compounds showed no significance increase in the frequency of polychromatic erythrocytes with micronuclei. These results suggest that the crude antifungal compounds produced by B. subtilis SN7 isolated from meju showed no harmful genotoxic effects.
Keywords
Bacillus subtilis SN7; crude antifungal compounds; bacterial reversion assay; chromosomal aberration test; micronucleus assay;
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Times Cited By KSCI : 4  (Citation Analysis)
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1 Ames BN, McCann J, Yamasaki E(1975) Methods for detecting carcinogens and mutagens with Salmonella/mammalian - microsome mutagenicity test. Mutation Res 31(6), 347-364   DOI
2 Bennett JW, Klich M(2003) Mycotoxins. Clin Microbiol Rev 16(3), 497-516   DOI
3 Chang HC, Koh SB, Lee JJ(2016) Single oral dose toxicity test and four weeks repeated oral dose determination test of crude antifungal compounds produced by Bacillus subtilis SN7 in rats. Korean J Community Living Sci 27(3), 437-449   DOI
4 Dean BJ, Danford N(1984) Assays for the detection of chemically-induced chromosome damage in cultured mammalian cells. In: Mutagenicity testing - a practical approach, Venitt S & Parry JM(Editors), IRL Press Limited, Eynsham, England pp187-232
5 Drider D, Fimland G, Hechard Y., McMullen LH, Prevost H(2006) The continuing story of class IIa bacteriocins. Microbiol Mol Biol Rev 70(2), 564-582   DOI
6 Hayashi M(1991) The micronucleus test. Science Press Inc., Tokyo, Japan. p 65-69
7 Hayashi M, Yoshimura I, Sofuni T, Ishidate M Jr(1989) A procedure for data analysis of the rodent micronucleus test involving a historical control. Environ Mol Mutagen 13(4), 347-356   DOI
8 Hong SG, Chung SG, Hyun SH(2008) The micronucleus test of the diglyceride preparation with conjugated linoleic acid by using mice. J Korean Soc Food Sci Nutr 37(7), 853-857   DOI
9 Huang YH, Jung DW, Kang IJ(2014) Genotoxicity safety evaluation of imported oranges irradiated with ionizing energy. J Korean Soc Food Sci Nutr 43(6), 909-915   DOI
10 Ishidate M Jr, Sofuni T, Yoshikawa K(1981) Chromosomal aberration tests in vitro as a primary screening tool for environmental mutagens and/or carcinogens. Gann Monogr Cancer Res 27(1), 95-107
11 Jack RW, Tagg JR, Ray B(1995) Bacteriocin of grampositive bacteria. Microbiol Rev 59(2), 171-200
12 Kim KH, Jeong CH, Joo SJ, Park JH, Moon JY, Cho EJ, Lee HT, Kwon HJ, Kim BW, Eom SH, Lee EW(2015) Single dose oral toxicity of Baciluus subtilis JNS in ICR mice. J Korean Soc Food Sci Nutr 44(1), 24-28   DOI
13 Melin P, Sundh I, Hakansson S, Schnurer J(2007) Biological preservation of plant derived animal feed with antifungal microorganisms: safety and formulation aspects. Biotechnol Lett 29(8), 1147-1154   DOI
14 Lee SG(2014) Isolation and characterization of Baciluus subtilis harboring anti-Baciluus cereus activity from meju and purification of its antimicrobial compound. MS Dissertation, Chosun university
15 Lee GG, Lee HD, Lee CH(2003) Changes in sensory characteristics during salt aging of doenjang (fermented soybean paste) made by different starters. Food Eng Prog 7(1), 13-19
16 Lim JH, Park BK, Kim MS, Rhee MH, Park SC, Yun HI(2004) Acute oral toxicity and pathogenicity of a potential probiotic Bacillus sp. A9184 isolated from soybean paste. J Toxicol Pub Health 20(4), 359-363
17 Maron, DM, Ames BN(1983) Revised methods for the Salmonella mutagenicity test. Mutat Res 113(3-4): 173-215   DOI
18 Schallmey M, Singh A, Ward OP(2004) Development in the use of Bacillus species for industrial production. Can J Microbiol 50(1), 1-17   DOI
19 Ministry of Food and Drug Safety(2014) Guideline for toxicity tests of drugs and related materials notification. Chungbuk, MFDS
20 Moss MO (2008) Fungi, quality and safety issues in fresh fruits and vegetables. J Appl Microbiol 104(5), 1239-1243   DOI
21 Sharp RJ, Scawen MD, Atkinson T(1989) Fermentation and downstream processing of Baciluus. Biotechnology Handbooks 2. Springer US, New York, USA pp255-292
22 Song HP, Shin EH, Yun H, Cho C, Kim D(2009) Establishing the genotoxicological safety of gammairradiated egg white and yolk. Korean J Food Preserv 16(5), 782-788
23 Tagg JR, Dajani AS, Wannamaker LW(1976) Bacteriocins of gram-positive bacteria. Bacteriol Rev 40(3), 722-756