Browse > Article

The Antimicrobial Activity against Helicobacter pylori and Antioxidant effect from the extracts of Mulberry leaves (Morus Alba L.)  

Cho, Young-Je (Department of Food Engineering, Sangju National University)
Ju, In-Sik (Department of Food Engineering, Sangju National University)
Kim, Byung-Oh (Department of Applied Biology, Sangju National University)
Kim, Jeung-Hoan (NIP Biotech.)
Lee, Byung-Gu (Department of Culinary & Bakery, Yuengnam Foreign Language College)
An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Haany University)
Choo, Jae-Weon (Sericulture & Entomology Experiment Station, Gyeongbuk Agricultural Technology Administration)
Publication Information
Applied Biological Chemistry / v.50, no.4, 2007 , pp. 334-343 More about this Journal
Abstract
The water extracts and 80% ethanol extracts from mulberry leaves of 108 kinds were tested their antimicrobial activities against Helicobacter pylori and antioxidant effects. The ABTS [2,2'-azino-bis(3-ethylbenzothiaznoline-6-sulfornic acid)] radical decolorization, electron donating ability (EDA), thiobarbituric acid reactive substance (TBARS) and antioxidant protection factor (PF) were determined for water extracts and 80% ethanol extracts from mulberry leaves. In the electron donating activity, the 13 kinds of water extracts showed high inhibition (>80%), whereas the 59 kinds of 80% ethanol extracts showed high inhibition. The inhibitory activities of water extracts from all kinds of mulberry leaves were higher than 90% in ABTS [2,2'-azinobis(3-ethylbenzothiaznoline-6-sulfornic acid)] radical decolorization. The thiobarbituric acid reactive substance (TBARS) and antioxidant protection factor (PF) of the 80% ethanol extracts were higher than that of water extracts. Antimicrobial activity against Helicobacter pylori showed high value in 80% ethanol extracts of 15 kinds mulberry leaves. The results implied that the mulberry leaves can be useful for natural antimicrobial medicine.
Keywords
Mulberry leaves; Helicobacter pylori; antimicrobial activity; antioxidant;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Lee, W. C., Kim, A. J. and Kim, S. Y. (2003) The study on the Functional materials and Effects of Mulberry leaf. Food Sci. and Indust. 36, 2-14
2 Satoh, K., Kimura, K., Taniguchi, Y., Yoshida, Y., Kihira, K., Takimoto, T., Kawata, H., Saifuku, K., Ido, K., Takemoto, T., Ota, Y., Tada, M., Karita, M., Sakaki, N. and Hoshihara, Y. (1996) Distribution of inflammation and atrophy in the stomach of Helicobacter pylori.-positive and -negative patients with chronic gastritis. Am. J. Gastroenterol. 91, 963-969
3 Blois, M. S. (1958) Antioxidant determination by the use of stable free radical. Nature 26, 1198-1199
4 Gavidson, P. H. and Parish, M. E. (1989) Methods for testing the efficacy of food antimicrobals. Food Technol. 43, 148-150
5 Diker, K. S. and Hascelik, G. (1994) The bactericidal activity of tea against Helicobacter pylori. Letters in Appl. Microbiol. 19, 299-300   DOI   ScienceOn
6 Cushman, D. W. and Ondetti, M. A. (1980) Inhibitors of angiotensin converting enzyme for treatment of hypertension. Biochem. Pharmacol. 29, 1871-1877   DOI   ScienceOn
7 Lee, S. E., Bang, J. K., Song, J., Seong, N. S., Park, H. W., Chung, H. G., Kim, G. S. and An, T. J. (2004) Inhibitory Activity on Angiotensin Converting Enzyme (ACE) of Korean Medicinal Herbs. Korean J. Medcinal Crop Sci. 12, 73-78
8 Goodwin, C. S. and Worsiey, B. W. (1993) Helicobacter pylori: Biology and clinical ratice. CPC press, Inc. USA
9 Lee, S. H., Lim, C. Y., Lee, K. H., Yeo, S. J., Kim, B. J., Kim, S. J., Cho, M. J., Rhee, K. H. and Kook, Y. H. (1999) rpoB Gene analysis of Helicobacter pylori. J. Kor. Soc. Microbiol. 34, 401-408
10 Kono, Y., Kashone, S., Yoneyama, T., Sakamoto, Y., Matsu, Y. and Shibata, H. (1998) Iron chelation by chlorogenic acid as a natural antioxidant. Biosci. Biotechnol. Biochem. 62, 22-27   DOI   ScienceOn
11 An, B. J., Lee, J. T., Lee, S. A., Kwak, J. H., Park, J. M., Lee, J. Y. and Son, J. H. (2004) Antioxidant effects and applocation as natural ingredients of Korean sanguisorbae officinalis. J. Kor. Soc. Appl. Biol. Chem. 47, 244-250
12 Borody, T., Lene, J. and Moore-Jones, D. (1990) Is doxycycline more effective than eracycline HCL in triple herapy of Helicobacter pylori. Gastroenterol. 98, 24-29
13 Marshall, B. J. and Warren, J. R. (1984) Unidentified curved bacilli in the stomach of patients with gastric and peptic ulceration. Lancet. 1, 1311-1315
14 Hirayama, F., Takagi, S., Yokiyama, Y., Iwao, E. and Ikeda, Y. (1996) Establishment of gastric Helicobacter pylori onfection in Mongolian gerbils. J. Gastroenterol. 31, 24-28   DOI   ScienceOn
15 Shin, D. H. (1996) The trend and direction of natural antioxidants research. Food Sci Ind. 30, 4-21
16 Aruoma, O. I. (1998) Free radical, oxidative stress and antioxidants in human health and disease. J. Am. Oil chem. Soc. 75, 199-212   DOI   ScienceOn
17 Kim, S. Y., Ryu, K. S., Lee, W. C., Ku, H. O., Lee, H. S. and Lee, K. R. (1999) ypoglycemic Effect of Mulberry Leaves with Anaerobic Treatment in Alloxan-induced Diabetic Mice. Kor. J. Pharmacogn. 30, 123-129
18 Kyrtopoulos, S. A. (1989) N-nitroso compound formation in human gastric juice. Cancer Surv. 8, 423-442
19 Park, C. K., Choi, H. J., Youn, H. S., Lee, W. K., Cho, N. J., Kang, K. H., Baik, S. C., Rhee, K. H. (1994) Chemotherapy of Helicobacter pylori infection. J. Kor. Soc. Microbiol. 29, 421-435
20 Hahm, K. B., Lee, K. M., Kim, Y. B., Han, S. U. and Kim, M. W. (2001) Animal models of Helicobacter pylori infection. Kor. J. Gastroenterol. 37, 399-405
21 Fridovich, I. (1986) Biological effects of the superoxide radical. Arch. Biochem. Biophys. 247, 1-15   DOI   ScienceOn
22 Andarwulan, N. and Shetty, K. (1999) Phenolic content in differentiated tissue cultures of untransformed and Agrobacterium transformed roots of anise (pimpinella anisum L.). J. Agric. Food Chem. 47, 1776-1780   DOI   ScienceOn
23 Hunt, R. H. (1996) Eradication of Helicobacter pylori infection. Am. J. Med. 100, 425-505
24 Pellegrin, N., Roberta, R., Min, Y. and Catherine, R. E. (1998) Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activites applying 2,2'-azinobis(3-ethylenebenzothiazoline-6-sulfonic acid) radical cation decolorization assay. Method Enzymol. 299, 379-389
25 Kim, K. S., Shim, S. H., Jeong, G. H. and Cheong, C. S. (1998) Antidiabetic activity of constituents of Lycii fructus. J. of Appl. Pharmacol. 6, 378-382
26 Robert, I. L. (1994) In Functional Foods Phytochemicals and antioxidants. Goldberg, I. (ed.) Chapman & Hall, New York. p.393
27 Hertong, M. C. L., Feskens, E. J. M., Hollman, P. C. H., Katan, M. B. and Kromhout, D. (1993) Dietary antioxidant flavonoids and risk of coronary heart disease. Lancet. 342, 1007-1011
28 Kim, J. H., Yoon, S. J., Cho, Y. J. (2005) Antimicrobial Activity against Helicobacter pylori and Antioxidant Activity of Yerusalem sage (phlomis frutcosa L.). J. Kor. Soc. Appl. Biol. Chem. 48, 178-182   과학기술학회마을
29 Buege, J. A. and Aust, S. D. (1978) Microsomal lipid peroxidation. Method Enzymol. 105, 302-310
30 Rauwa, E. A.J., Langenberg, W., Hoythoff, J. H., Zanen, H. C. and tytgt, G. H. J. (1988) Campylobacter pyloridis-associated chronic active antral gastritis: a proseoective study of its prevelance and the effects of anthibacterial and aniyclera prevelance and the effects of aninacterial and antiuclera treatment. Gasreryerol. 94, 33-40