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Isolation of N-Iauroyl Tyrosine Antibiotic in E. coli Carrying N-acyl Amino Acid Synthase Gene from Environmental DNA in Korean Soils  

Yeo, Yun-Soo (Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration)
Lim, Yoon-Ho (Department of Bioscience and Biotechnology, Konkuk University)
Kim, Jeong-Bong (Molecular Physiology Division, National Institute of Agricultural Biotechnology, Rural Development Administration)
Yang, Jung-Mo (Department of Bioscience and Biotechnology, Konkuk University)
Lee, Chang-Muk (Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration)
Kim, Soo-Jin (Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration)
Park, Min-Seon (Department of Biochemistry and Molecular Biology, Ajou University School of Medicine)
Koo, Bon-Sung (Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration)
Yoon, Sang-Hong (Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration)
Publication Information
Applied Biological Chemistry / v.50, no.4, 2007 , pp. 262-267 More about this Journal
Abstract
To access the natural product antibiotics produced by uncultured microorganisms, six cosmid libraries of DNA extracted directly from soil samples (environmental DNA, eDNA) were constructed and screened for the production of antibacterial active molecules. Of the approximately 60,000 clones screened, one antibacterial clone (YS92B) was detected. Ethyl acetate extracts of clone YS92B showed antibacterial activity against various pathogenic bacteria (Listeria monocytogenes, Bacillus subtilis, Pseudomonas syringae, Xanthomonas campestris pv. oryzae, Staphylococcus epidemis). Active constituents from cultures of YS92B were isolated and purified using a bioassay-guided fractionation against B. subtilis through a series of procedures (ethyl acetate extraction, Sephadex LH20 column chromatography, High Performance Liquid Chromatography). NMR (Nuclear Magnetic Resonance) spectral analysis of a major antibacterial active YS92B-VII indicated that it is a lauric acid linked to tyrosine. This report describes the characterization of antibacterially active long chain N-acyl derivatives of tyrosine that are produced by eDNA clones hosted in Escherichia coli from Korean soils.
Keywords
eDNA; metagenomic library; N-acyl tyrosine antibiotics;
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