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Morphological Characterization of 'Ailsa Craig' Tomato Callus and Effect on Plant Regeneration  

Seong, Eun-Soo (School of Biotechnology, Kangwon Naitonal University)
Wang, Myeong-Hyeon (School of Biotechnology, Kangwon Naitonal University)
Publication Information
Applied Biological Chemistry / v.50, no.4, 2007 , pp. 253-256 More about this Journal
Abstract
In an attempt to optimize the in vitro-regeneration conditions necessary for the genetic manipulation of tomato species, we examined 'Ailsa Graig' cultivar of Lycopersicon for regeneration ability. The basal medium used for callus formation and shoot regeneration was MS (MS + vitamin) supplemented with six combinations of zeatin 2 mg/l, zeatin 2 mg/l + IAA 0.1 mg/l, zeatin 2 mg/l + IAA 0.5 mg/l, zeatin 4 mg/l, zeatin 4 mg/l + IAA 0.1 mg/l and zeatin 4 mg/l + IAA 0.5 mg/l. When all conditions tested were considered, however, only zeatin 2 mg/l was shown to be the best in shoot regeneration. The morphological characterization from in vitro-cultured callus of Lycopersicon esculentum L. var. 'Ailsa Craig' was investigated with scanning electron microscope (SEM). The surfaces of in vitro-cultured callus had well-defined epidermal cell in condition of zeatin 2 mg/l, but those of different treatments were twisted. These results suggested that shape of callus was involved in efficiency of shoot regeneration in tomato 'Ailsa Craig'.
Keywords
Ailsa Craig; SEM; shoot regeneration; zeatin;
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