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Physiological Characteristics of Biosurfactant-Producting Bacillus subtilis TBM 3101  

Kim, Seon-A (Department of Biology, Changwon National University)
Lee, Young-Guen (School of Applied Life Science, Pusan National University)
Choi, Yong-Lark (Department of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University)
Hwang, Cher-Won (Institute of Bioscience and Technology, Handong University)
Jeong, Yong-Kee (Department of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University)
Joo, Woo-Hong (Department of Biology, Changwon National University)
Publication Information
Applied Biological Chemistry / v.50, no.1, 2007 , pp. 12-17 More about this Journal
Abstract
A biosurfactant-producing strain, Bacillus subtilis TBM 3101 was isolated from the soil sample at Tae-Baek Mountain through an antifungal test and emulsification assessment. The strain was assessed, regarding to the microbial growth, by physical and chemical test, surface tension, emulsification activity and stability. The surface tension of the isolate sharply decreased to the minimum 29mN/m at 48 h growth. Of note, its emulsification was stabilized to the highest degree when tributyrin was utilized as a substrate, indicating that in comparison to a variety of synthetic surfactants, the biosurfactant produced by the isolate was significantly similar to synthetic surfactant, tween 20. In addition, the biosurfactant showed high emulsification activity when soybean oil, crude oil and tetradecane were used as a substrate. Thus, these studies could contribute to the detection and development of biosurfactant beneficial to the environment and humans.
Keywords
Bacillus subtilis; biosurfactant; emulsifying activity; emulsifying stability;
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