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A Study on Hypoglycemic Effects of Crude Extracts of Schizandrae Fructus  

Ko, Byoung-Seob (Korea Institute of Oriental Medicine)
Park, Seong-Kyu (College of Oriental Medicine, Kyung Hee University)
Choi, Soo-Bong (School of Medicine, Konkuk University)
Jun, Dong-Wha (Food and Nutrition, Hoseo University)
Choi, Mi-Kyung (Food and Nutrition, Hoseo University)
Park, Sun-Min (Food and Nutrition, Hoseo University)
Publication Information
Applied Biological Chemistry / v.47, no.2, 2004 , pp. 258-264 More about this Journal
Abstract
Hypoglycemic effect of Schizandrae Fructus (SF) extract containing in Okchun-san was determined on 3T3-L1 fibroblasts and adipocytes by investigating insulin-like activity, insulin sensitizing activity and ${\alpha}-glucoamylase$ suppressing activity. SF were extracted by using 70% ethanol followed by XAD-4 column chromatography with a mixture solvent of methanol and water, and the fractional extractions were utilized for assaying hypoglycemic effect. No inhibition of ${\alpha}-glucoamylase$ activity of SF was observed. Insulin-like activity 3T3-L1 adipocytes was not shown by SF. A significant insulin sensitizing activity of SF extractions was observed in 3T3-L1 adipocytes, giving SF extractions with 1 ng/ml insulin to reach glucose uptake level increased by 50 ng/ml of insulin alone. When cells were treated with SF (Fr. 4 or 5) plus 1 ng/ml insulin, glucose uptake was increased more than seven times as compared to 1 ng/ml of insulin alone, suggesting that SF extracts increased GLUT4 content by enhancing insulin signaling. These data suggest that SF extracts (especially Fr. 4 and 5) contains an effective insulin sensitizing compounds for hypoglycemic activity in 3T3-L1 adipocytes.
Keywords
insulin sensitizer; insulin-like substance; ${\alpha}-glucoamylase$; Okchun-san(OCH); 3T3-L1 adipocytes;
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