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Discrimination of Geographical Origin far Ligusticum Root (Ligusticum wallichii) by Capillary Electrophoresis  

Kim, Jung-Hyun (Korea Food Research Institute)
Kim, Eun-Young (Korea Food Research Institute)
Chung, Kyung-Sook (Korea Food Research Institute)
Rhyu, Mee-Ra (Korea Food Research Institute)
Publication Information
Applied Biological Chemistry / v.46, no.4, 2003 , pp. 380-384 More about this Journal
Abstract
Optimal extraction, separation and capillary rinsing conditions for capillary electrophoresis (CE) were established to discriminate the geographical origin of ligusticum root (Ligusticum wallichii) using 113 samples (domestic sample n = 62, foreign sample n = 51). Ligusticum root was extracted with 30% ethanol and separated on a uncoated fused-silica $(50\;{\mu}m{\times}27\;cm)$ capillary. Conditions for optimal analysis include: temperature, $40^{\circ}C$; voltage, 10 kV; and pressure injection time, domestic and foreign samples were 5 sec and 2 sec, respectively. The optimal separation buffer was 0.1 M phosphate buffer (pH 2.5) containing 15 mM iminodiacetic acid with 40% methanol. Under the optimal conditions established for CE, the ratio of specific peak area (peak LW-1) to other peak area (peak LW-5) was effective in discrimination geographical origin of ligusticum root. The mean accuracy for correct discrimination of geographical origin of domestic and foreign ligusticum roots were 65% and 63%, respectively.
Keywords
capillary electrophoresis; geographical origin; Ligusticum root; conditions for optimal analysis;
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