Browse > Article

Antioxidantive, Phospholipase $A_2$ Inhibiting, and Anticancer Effect of Polyphenol Rich Fractions from Panax ginseng C. A. Meyer  

Choi, Hee-Jin (Department of Food Science & Technology College of Natural resources Yeungnam University)
Han, Ho-Suk (Department of Food Science & Technology College of Natural resources Yeungnam University)
Park, Jung-Hye (Department of Food Science & Technology College of Natural resources Yeungnam University)
Son, Jun-Ho (Korea Advanced Food Research Institute)
Bae, Jong-Ho (Department of Confectionery Decoration, Daegue Mirae College)
Seung, Tae-Su (Department of Food & Nutrition, Changwon Junior College)
Choi, Cheong (Department of Food Science & Technology College of Natural resources Yeungnam University)
Publication Information
Applied Biological Chemistry / v.46, no.3, 2003 , pp. 251-256 More about this Journal
Abstract
The polyphenol fractions of Korean ginseng were purified using Sephadex LH-20, MCI gel, Bondapak $C_{18}$ TLC, and HPLC from the 60% acetone soluble fraction. Fraction I showed 48.16%, 79.71% and 43.55% inhibition at 150 ppm against lipid oxidation in the presence of copper ion, superoxide and hydrogen peroxidation. Electron donating abilities of fraction II showed 35.17% inhibition at 200 ppm. Fraction III showed 48.49% and 25% inhibition at 150 ppm against lipid oxidation in the presence of ferrous ion and hydroxy radical ion. The phospholipase $A_2$ inhibitory effect of fraction III was 48.9% at the concentration of $60\;{\mu}g/ml$. The cytotoxic effects of fraction II was the highest (73.29% at 0.25 mg/ml) among the tested polyphenol fractions.
Keywords
ginseng; polyphenol fraction; antioxidant; phospholipase $A_2$ inhibiting activity; anticancer;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Korea ginseng. (1993) In AnnuaI Report of Korean ginseng & tobacco research institute, pp. 1-6
2 Park, C. W. (1984) The studies of pharmacology of ginseng. Biochemistry News 4, 37-56
3 Lee, H. O. and Park, O. J. (1998) Antioxidant effects of phenolic acid and ginseng extract in aqueous system. Korean J. Food Sci. Technol. 30, 34-438
4 Han, B. H. and Park, M. H. (1978) Studies on the antioxidant components of Korean ginseng (II). Biochem. J. 9, 169-171
5 Kawashima, S. (1969) The possible role of lipoperoxide in aging. Nagoya J. Med. Sci. 32, 303-326
6 Tien, M. and Aust, S. D. (1981) Superoxide dependent lipid peroxidation. Fed. Proc. 40, 179-182
7 Fridovich, I. (1986) Biological effect of the superoxide radical irwin fridovich. Arch. Biochem. Biophy. 247, 1-11   DOI   PUBMED   ScienceOn
8 Tanaka, K., Aiita, H. and Inoue, K. (1993) Phospholipase $A_2$ inhibitiors. Prot. Nucl. Acid Enzym. 38, 1988-1999
9 Hwang, W. I. and Soo, K. O. (1984) A study on the anticancer actives of lipid soluble ginseng extract and ginseng saponin derivatives against some cancer cells. Korean J. Ginseng Sci. 8, 153-166
10 Kim, S. I. (1988) Studies on the cytotoxic components of the Korean ginseng root, Ph. D. Thesis, Chungnam National University, Daejeon, Korea
11 Buege, J. A. and Aust, S. D. (1978) Microsomal lipid peroxidation. Methods Enzymol. 105, 302-305
12 Blois, M. S. (1958) Antioxidant determination by the use of a stable free radical. Nature 26, 1198-1199
13 Dole, V. P. and Meinertz, H. (1960) Microdetermination of long-chain fatty acids in plasma and tissues. J. Biol. Chem. 235, 2595-2599
14 Dariusz, S., Sarah, J. S. Richard, H. C. and Michael, B. (1960) An improved MTT assay. J. Immuno. Methods 157, 203-207
15 Hwang, W. I. and Cha, S. M. (1978) A cytotoxic activity of extract of panax ginseng root aginst some cancer cell. In proc. 2nd Int. Ginseng Symp. pp. 47-52
16 Hwang, W. I. and Oh, S. K. (1984) A study on the anticancer actives of lipid soluble ginseng extract and ginseng saponin derivatives against some cancer cells. Korean J. Ginseng Sci. 8, 153-166
17 Hagiwara, N., Tateishi, M., Kim, M., Yamane, T. and Takashi, T. (1994) In Proceeding of the international symposium on tea science: Inhibition of axoxymethane-mduced colon carcinogensis in rat by tea polyphenols. Seoul, Korea. pp. 190-194
18 Brekhman, I. I. (1957) Gosudarst Isdat et Med. Lit. Leningrad. 1-18
19 Han, B. H., Park, M. H., Woo, L. K., Woo, W. S. and Han, Y. N. (1979) Studies on the antioxidant components of Korean ginseng (I). Korean Biochem. J. 12, 33-40
20 Hwang, W. I. and Soo, K. O. (1986) Effects of petroleum extract of ginseng root on some enzyme activity in human colon cancer cells. Korean J. Ginseng Sci. 10, 27-35
21 Lee, S. H. and Hwang, W. I. (1986) Inhibitory effect of petroleum ether extract of panax ginseng root against growth of human cancer cells. Korean J. Ginseng Sci. 10, 141-150
22 Jin, H. K., Kim, S. H. and Lee, J. K. (1982) Studies of the physiologcal activity of Korean ginseng. Kor. J. Appl. Microbiol. Bioeng. 10, 101-108
23 Choi, H. J., Zhang, Y. B., An, B. J. and Choi, C. (2002) Identification of biologically active compounds from panax gingeng C. A. Meyer. Korean J. Food Sci. Technol. 34, 493-497
24 Park, T. H., Hong, J. T. and Hong, S. Y. (1982) Studies oh the antioxygenic substances in panxa gingeng roots. Korean Food Sci. Technol. 14, 130-135
25 Fricovich, I. (1989) Superoxide dimutases. J. BioI. Chem. 264, 7761-7764
26 Wee, J. J. (1989) Isolation and identification ofconsdtuents from antioxidant and hematopoietic fracdons of panax ginseng C. A. Meyer. Ph. D. Thesis, Seoul National University, Seoul, Korea
27 Babizhayev, M. A., Seguin, M. C., Gueyne, J., Evstigneeva, R. P., Ageyeva, E. A. and Zheltukhina, G. A. (1994) L-camosine ($\beta$-alanyl-L-histidine) and carcinine ($\beta$-alanylhistamine) act as natural antioxidants with hydroxyl radical-scavenging and lipid-peroxidase activities. Biochem. J. 304, 509-913   DOI
28 Kim, S. M., Cho, Y. S. and Sung, S. K. (1999) Effect of ethonol extracts in pinus densiflora, lithospermum erythrohizon on the lipid oxidation of oil emulsion. J. Korean Soc. Food Sci. Nutr. 28, 984-989
29 Han, B. H. and Park, M. H. (1978) Studies on the antioxidant components of Korean ginseng (II). 9, 169-171
30 Hwang, W. I. and Oh, S. K. (1984) A study on the anticancer acitvites of lipid soluble ginseng extract and ginseng saponin derivatives against some cancer cells. Korean J. Ginseng Sci. 8, 153-166
31 Masataka, Y. and Keiko, M. (1998) Interaction of iron with polyphenolic compounds, application to antioxidant characterization. Anal. Biochem. 257, 40-44   DOI   ScienceOn
32 Addis, P. B. (1986) Occurrence of lipid oxidation products in foods. Food Chem. Toxicol. 24, 1021-1030   DOI
33 Chan, W. K. M., Decker, E. A., Lee, J. B. and Butterfield, D. A. (1994) EPR spintrapping studies of the hydroxyl radical scavening activity of carnosine and related dipeptides. J. Agric. Food Chem. 42, 1407-1410   DOI   ScienceOn
34 Dennis, E. A. and Boyer, P. D. (1983) The enzymes. Academic press, New York. 16, pp. 307-353
35 Park, J. D., Kim, M. W. and Wee, J. J. (1987) Isolation and identification of free phenolic acids in Korean ginseng. Korean J. Food Sci. Technol. 19, 392-396
36 Harman, D. Aging. (1956) A theory based on free radical and radiation chemistry. J. GervntoI 11, 298-300
37 Kim, J. W., Song, K. S., Chang, H. W., Yu, S. H. and Yoo, I. D. (1995) A phospho-lipase $A_2$, inhibitior isolated from umgilicaria esculenta. Kor. J. Appl. Microbiol. Biotechnol. 23, 526-530
38 Lee, J. W., Do, J. H. and Shim, K. H. (1999) Antioxidant activity of the water soluble brewing reaction prducts isolated from Korean red scavenging. J. Ginseng Res. 23, 176-181
39 Lee, S. H. and Min, D. B. (1990) Effects, quenching mechanisms and kinetics of carofenoids in chlorophlly sensitized photooxidation of soybean oil. J. Agric Food Chem. 38, 1630-1634   DOI
40 Thomas, C., Glenn, F. V. and Christione, C. W. (1993) The hydrolysis product of icre-187 promotes iron-catalysed hydroxylradical production via the fenton reaction. Biochem. Pharmacol. 15, 1967-1972
41 Kim, J. W., Song, K. S., Yoo, I. D., Chang, H. W., Yu, S. H., Bae, K. G. and Min, T. J. (1996) Two phenolic compounds isolated from umgilicaria esculenta as phospholipase $A_2$inhibitors. Korean J. Mycotogy 24, 237-242
42 Kim, J. W., Song, K. S. and Chang, H. W. (1995) A phospholipase $A_2$ inhibitor isolated form umbilicaria esclenta. Kor. J. Appl. Microbiol. Biotechnol. 23, 526-530
43 Han, B. H., Park, M. H. and Han, Han, Y. N. (1985) Studies on the antioxidant components of Korean ginseng. Korean Biochem. J. 18, 337-340
44 Garriques, S. S. (1854) On panaquilon, a new vegetable substance. Ann. Chem. Pharm. 40, 231-233
45 Park, M. H. (1998) EfFect of polyphenol compounds from persimmon leaves (Diospyros kaki folium) on immunofunctional and biological activity, Ph. D. Thesis, Yeungnam University, Kyungsan, Korea
46 Han, B. H., Park, M. H. and Han, Y. N. (1985) Studies on antioxidant components of Korean ginseng (V): The mechanism of antioxidant activity of maltol and phenolic acid. Korean Biochem. J. 18, 337-340
47 Lee, H. O. and Park, O. J. (1998) Antioxidant effects of phenolic acid and ginseng extract in aqueous system. Korean J. Food Sci. Technol. 30, 434-438
48 Kim, M. W., Choi, Y. J., Cho, Y. H. and Hong, S. K. (1980) Study on the components of the anti-oxidant activity of panax ginseng. J. Korean Agri. Chem. Soci. 23, 173-177   과학기술학회마을
49 Han, B. H. (1972) Current status of Korean ginseng research. Kor. J. Pharmacog. 3, 151-160
50 Minotti, G. and Ausst, S. D. (1987) The requirement for iron (III) in the initation of lopod peroxidadon by iron (II) and hydrogen peroxide. J. Biol. Chem. 262, 1098-1104
51 Han, B. H., Park, M. H and Han, Y. N. (1985) Studies on antioxidant components of Korean ginseng (V). The mechanism of antioxidant activity of maltol and phenolic acid. Korean Biochem. J. 18, 337-340
52 Hwang, W. I. and Oh, S. K. (1996) Effects of petroleum extract of ginseng root on some enzyme activity in human colon cancer cell. Korean J. Ginseng Sci. 10, 27-35
53 Dicker, E. A., Cmm, A. D. and Calvert, J. T. (1992) Differences in the antioxidant mechanism of carmosine in the prescence of copper and iron. J. Agric. Food Chem. 40, 756-759   DOI
54 Han, B. H., Park, M. H., Woo, L. K., Woo, W. S. and Han, Y. N. (1979) Studies on the antioxidant components of Korean ginseng (I). Korean Biochem. J. 12, 33-40
55 Kang, Y. H., Park, Y. K. and Lee, G. D. (1996) The nitric scavenging and electron donating ability of phenolic compounds. Korean J. Food Sci. Technol. 28, 232-239
56 Ko, S. R. (1993) Comparative study onchemical components and biological activities of panax species, Ph. D. Thesis, Chonbuk National Unuversity, Chonju, Korea