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http://dx.doi.org/10.22424/jmsb.2018.36.1.39

Immunomodulatory Effects of Fermented Milk based on Synbiotic Interaction between Cudrania tricuspidata Leaf Extract and Lactobacillus gasseri 505  

Lee, Ji Young (R&D Center, Seoul Dairy Cooperative)
Kim, Su Gyeong (R&D Center, Seoul Dairy Cooperative)
Shin, Yong Kook (R&D Center, Seoul Dairy Cooperative)
Oh, Nam Su (R&D Center, Seoul Dairy Cooperative)
Publication Information
Journal of Dairy Science and Biotechnology / v.36, no.1, 2018 , pp. 39-48 More about this Journal
Abstract
This study examined the immunomodulatory effects of milk fermented with based on the synbiotic interaction between Cudrania tricuspidata leaf extract (CT) and Lactobacillus gasseri 505. Raw 264.7 macrophages were treated with milk (Control), milk supplemented with 0.2% CT (CT-milk), or CT-milk fermented with L. gasseri 505 (FCT). FCT-treated cells showed higher NO production than the other groups. ELISA revealed that FCT produced the highest enhancement of the production of pro-inflammatory cytokines such as $TNF-{\alpha}$, $IFN-{\gamma}$, $IL-1{\beta}$, IL-6, IL-2, IL-12, and IL-10. Furthermore, FCT also increased the gene expression of pro-inflammatory mediators such as iNOS and COX-2 in a concentration-dependent manner. Collectively, these results indicate that the synbiotic interaction between CT and L. gasseri 505 might enhance their immunomodulatory effects of fermented milk.
Keywords
Lactobacillus gasseri; Cudrania tricuspidata; immunomodulatory activity; cytokines;
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