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HPLC-based Analysis of Biogenic Amines in Aging-Cheese  

Park, Jong-Hyuk (Imsil Research Institute of Cheese Science)
Lee, Sang-Cheon (Imsil Research Institute of Cheese Science)
Moon, Hye-Jung (Imsil Research Institute of Cheese Science)
Oh, Jeon-Hui (Imsil Research Institute of Cheese Science)
Song, Gi-Bong (Eple Farming Association Corporation)
Publication Information
Journal of Dairy Science and Biotechnology / v.34, no.3, 2016 , pp. 187-191 More about this Journal
Abstract
Biogenic amines have been used as chemical indicators of fermented foods. So far, several chromatography methods have been developed to detect biogenic amines in foods. However, few methods have identified these compound in domestic cheese. We analyzed the biogenic amines (histamine dihydrochloride, tyramine hydrochloride, ${\beta}$-phenylethylamine hydrochloride, putrescine dihydrochloride, cadaverine, spermidine, tryptamine hydrochloride, ethanolamine hydrochloride and butylamine) in cheese by using HPLC. The calibration curves of the biogenic amines were found to be linear over the concentration range of 10-50 ppm with a correlation coefficient of above 0.99. The limit of detection (LOD) and limit of quantitation (LOQ) of the biogenic amines in the given order were 3.7 and 11.3 ppm, 3.4 and 10.4ppm, 3.4 and 10.3 ppm, 4.0 and 12.2 ppm, 3.4 and 10.4 ppm, 3.4 and 10.5 ppm, 3.5 and 10.7 ppm, 4.1 and 12.5 ppm, and 3.4 and 10.4 ppm, respectively. Recovery rates of the biogenic amines in the given order were 112, 104, 93, 108, 91, 102, 101, and 92%, respectively. The findings of this study suggest that HPLC is a suitable method for the determination of biogenic amines, thereby indicating its potential application in the quality control of aging cheese.
Keywords
biogenic amine; aging cheese; HPLC;
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