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http://dx.doi.org/10.7585/kjps.2012.16.3.242

Modified QuEChERS Multi-Residue Analysis Method for 61 pesticides in Fruits using with HPLC and GC-ECD/NPD  

Lee, Ju-Young (National Instrumentation Center for Environmental Management, Seoul National University)
Hong, Su-Myeong (Department of Agro-food Safety, National Academy of Agriculture Science, Rural Development Administration)
Kim, Taek-Kyum (Department of Agro-food Safety, National Academy of Agriculture Science, Rural Development Administration)
Min, Zaw Win (Department of Agro-food Safety, National Academy of Agriculture Science, Rural Development Administration)
Kim, Yang-Hyeon (Croen research inc.)
Song, Kyung-Ae (Department of Agro-food Safety, National Academy of Agriculture Science, Rural Development Administration)
Kwon, Hye-Yong (Department of Agro-food Safety, National Academy of Agriculture Science, Rural Development Administration)
Lee, Hee-Dong (Department of Agro-food Safety, National Academy of Agriculture Science, Rural Development Administration)
Im, Geon-Jae (Department of Agro-food Safety, National Academy of Agriculture Science, Rural Development Administration)
Kim, Doo-Ho (Department of Agro-food Safety, National Academy of Agriculture Science, Rural Development Administration)
Kim, Jang-Eok (School of Applied Biosciences, Kyungpook National University)
Publication Information
The Korean Journal of Pesticide Science / v.16, no.3, 2012 , pp. 242-256 More about this Journal
Abstract
QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method has been a lot of research for pesticide analysis, because it is very simple and fast. However, this method requires high sensitivity instrument such as LC-MS/MS because of the use of small sample volume and many impurities compared to the conventional method. So, QuEChERS method needs to be modified for using with HPLC and GC-ECD/NPD. The aim of this work was to study the application of the QuEChERS method as well as its modification for the extraction and preconcentration of 5 groups of 61 pesticides from 4 fruits prior to their determination by HPLC-PDA, GC-ECD/NPD, and LC-MS/MS. The method was validated using spiking levels at 0.1 mg/kg (or 0.01 mg/kg) in apple, grapes, pear and persimmon. The average recovery by QuEChERS AOAC Official 2007. 01 version using the LC-MS/MS varied from 71.1127.4% for 61 pesticides. The average recovery rates using modified QuEChERS varied from 70.9~126% for 61 pesticides by HPLC-PDA and GC-ECD/NPD. The results satisfied the criteria of multiple pesticide residue analysis, setting 70~130% for recovery rates and below 30% for CV.
Keywords
QuEChERS; Residual Pesticides; HPLC-UVD; GC-ECD/NPD;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
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