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http://dx.doi.org/10.4014/mbl.1907.07013

Phylogenetic Diversity and Antibacterial Activity in Bacterium from Balloon Fish (Diodon holocanthus) of Jeju Island  

Moon, Chae-Yun (Department of Aquatic Biomedical Sciences, Jeju National University)
Ko, Jun-Cheol (Jeju Fisheries Research Institute, National Institute of Fisheries Science)
Kim, Min-Seon (Jeju Fisheries Research Institute, National Institute of Fisheries Science)
Heo, Moon-Soo (Department of Aquatic Biomedical Sciences, Jeju National University)
Publication Information
Microbiology and Biotechnology Letters / v.48, no.1, 2020 , pp. 57-63 More about this Journal
Abstract
Over the past 20 years, global warming has transformed the marine ecosystem of the Jeju Island into a subtropical zone making it conducive to the production of tropical fishes. Recently, the balloon fish (Diodon holoanthus) has been found off the coast of the Jeju Island. In this study, we analyzed the diversity of its intestinal microorganisms as a representative for the surrounding environment. In addition, the isolates were evaluated for their antibacterial activity. A total of 161 strains of various species were identified and isolated using 16S ribosomal RNA gene sequence analysis. They were separated into three groups, of which Phylum Proteobacteria was found to be the most dominant with 91% sequence similarity. This includes the class γ-proteobacteria that is made up of twelve genera and twenty-four hundred species. The second group comprised strains of the genus Vibrio, made up of 35% Photobacteria, 32% Shewanella, and 6% Psychrobacter. It was also determined that 4% of the isolates were Acinetobacter, 3% were Enterovibrio, while Moraxella_g2 accounted for 1% of the total isolates. Class α-proteobactera includes five genera and five species; Brevundimonas, Allorhizobium, Pseudoceanicola and Erythrobcter, each accounting for 1% of the total isolates. The Firmicute strains belonged to six genera and ten species. 5% of the strains were Terribacillus, while Paenibacillus, Salinicoccus, Staphylococcus and Streptococcus accounted for 1% each of the total isolates. Actinobacteria accounted for the final phylum with strains belonging to three genera and ten species with Janibacter, Micrococcus and Isoptericola each accounting for 1% of the total isolates.
Keywords
Diodon holoanthus; balloon fish; phylogenetic analysis; 16S rDNA; antibacterial activity;
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1 Bairagi A, Sarkar Ghosh K, Sen SK, Ray AK. 2004. Evaluation of the nutritive value of Leucaena leucocephala leaf meal, inoculated with fish intestinal bacteria Bacillus subtilis and Bacillus circulans in formulated diets for rohu, Labeo rohita (Hamilton) fingerlings. Aquac. Res. 35: 436-446.   DOI
2 Chaucheyras-Durand F, Durand H. 2010. Probiotics in animal nutrition and health. Benef. Microbes. 1: 3-9.   DOI
3 Daly K, Kelly J, Moran AW, Bristow R, Young IS, Cossins AR, et al. 2018. Host selectively contributes to shaping intestinal microbiota of carnivorous and omnivorous fish. J. Gen. Appl. Microbiol. 65: 129-136.
4 Deep K, Poddar A, Das SK. 2014. Photobacterium panuliri sp. nov., an alkalitolerant marine bacterium isolated from eggs of spiny lobster, Panulirus penicillatus from Andaman sea. Curr. Microbiol. 69: 660-668.   DOI
5 Fang Y, Wang Y, Liu Z, Lu B, Dai H, Kan B, et al. 2017. Shewanella carassii sp. nov., isolated from surface swabs of crucian carp and faeces of a diarrhea patient. Int. J. Syst. Evol. Microbiol. 67: 5284-5289.   DOI
6 Gomez GD, Balcazr JL. 2008. A review on the interactions between gut microbiota and innate immunity of fish. FEMS Immunol. Med. Microbiol. 52: 145-154.   DOI
7 Eigelsbach HT, Downs CM. 1961. Prophylactic effectiveness of live and killed tularemia vaccines. J. Immunol. 87: 415-425.
8 Hentschel U, Steinert M, Hacker J. 2000. Common molecular mechanisms of symbiosis and pathogenesis. Trends Microbiol. 8: 226-230.   DOI
9 Huang YR, Shiau CY, Chen HH, Huang BC. 2011. Isolation and characterization of acid and pepsin-solubilized collagens from the skin of ballon fish (Diodon holocanthus). Food. Hydrocoll. 25: 1507-1513.   DOI
10 Huang MM, Guo LL, Wu YH, Lai QL, Shao ZZ, Wang CS, et al. 2018. Pseudooceanicola lipolyticus sp. nov., a marine alphaproteobacterium, reclassification of Oceanicola flagellates as Pseudooceanicola flagellates comb. nov. and emended description of the genus Pseudooceanicola. Int. J. Syst. Evol. Microbiol. 68: 409-415.   DOI
11 Kim HS, Kim HS, Jung MM, Lee JB. 2013. New record of dinoflagellates around Jeju Island. J. Ecol. Environ. 36: 273-291.   DOI
12 Macian MC, Garay E, Grimont PAD, Pujalte MJ. 2004. Vibrio ponticus sp. nov., a neighbor of V. fluvialis-V. furnissii clade, isolated from Gilthead sea bream, mussels and seawater. Syst. Appl. Microbiol. 27: 535-540.   DOI
13 Kim MJ, Han HS, Kim JS, Kim BY, Song CB. 2014. Species composition and bimonthly changes of fish community in the coastal waters of sagyeoi, Jeju Island. Han. Gug. Eoryu. Hag. Hoeji. 26: 212-221.
14 Kim SW, Chung M, Park HS. 2015. Tropical fish species thriving in temperate korean waters. Mar. Biodivers. 45: 147-148.   DOI
15 Krahenbuhl JL, Humphres RC, Henika PC. 1982. Effects of Propionibacterium acnes treatment on the course of Mycobacterium leprae infection in mice. Infect. Immun. 37: 183-188.   DOI
16 Nayak SK. 2010. Role of gastrointestinal microbiota in fish. Aquac. Res. 41: 1553-1573.   DOI
17 Olsson JC, Westerdahl A, Conway PL, Kjelleberg S. 1992. Intestinal colonization potential of turbot (Scophthalmus maximus) and Dab (Limanda limanda)-associated bacteria with inhibitory effects against Vibrio anguillarum. Appl. Environ. Microbiol. 58: 551-556.   DOI
18 Ray AK, Bairagi A, Sarkar-Ghosh K. 2007. Optimization of fermentation conditions for cellulose production by Bacillus subtilis CY5 and Bacillus circulans TP3 isolated from fish gut. Acta. Ichthyol. Piscat. 37: 47-53.   DOI
19 Peces R, Gago E, Tejada F, Laures AS, Alvarez-Grande J. 1997. Relapsing bacteraemia due to Micrococcus luteus in a haemodialysis patient with a perm-cath catheter. Nephrol. Dial. Transplant. 12: 2428-2429.   DOI
20 Sudh Rani P, Saini MK, Pinnaka AK, Kumar GS, Kumar S, Vemuluri VR, et al. 2019. Shewanella submarina sp. nov., a gammaproteobacterium isolated from marine water. Int. J. Syst. Evol. Microbiol. 69: 39-45.   DOI
21 Sugita H, Shibuya K, Shimooka H, Deguchi Y. 1996. Antibacterial abilities of intestinal bacteria in freshwater cultured fish. Aquaculture 145: 195-203.   DOI
22 Sommer F, Anderson JM, Bharti R, Raes J, Rosenstiel P. 2017. The resilience of the intestinal microbiota influences health and disease. Nat. Rev. Microbiol. 15: 630-638.   DOI
23 Stephens WZ, Burns AR, Stagaman K, Wong S, Rawls JF, Guillemin K, et al. 2016. The composition of the zebrafish intestinal microbial community varies across development. ISME J. 10: 644-654.   DOI
24 Sugita H, Shibuya K, Hanada H, Deguchi Y. 1997. Antibacterial abilities of intestinal microflora of the river fish. Fish. Sci. 63: 378-383.   DOI
25 Tarnecki AM, Burgos FA, Ray CL, Arias CR. 2017. Fish intestinal microbiome: diversity and symbiosis unraveled by metagenomics. J. Appl. Microbiol. 123: 2-17.   DOI
26 Thompson FL, Hoste B, Thompson CC, Goris J, Gomez-Gil B, Huys L, et al. 2002. Enterovibrio norvegicus gen. nov., sp. nov., isolated from the gut of turbot (Scophthalmus maximus) larvae: a new member of the family Vibrionaceae. Int. J. Syst. Evol. Microbiol. 52: 2015-2022.   DOI
27 Yang G, Bao B, Peatman E, Li H, Huang L, Ren D. 2007. Analysis of the composition of the bacterial community in puffer fish Takifugu obscurus. Aquaculture 262: 183-191.   DOI
28 Thompson JR, Randa MA, Marcelino LA, Tomita-Mitchell A, Lim E, Polz MF. 2004. Diversity and dynamics of a north atlantic coastal Vibrio community. Appl. Environ. Microbiol. 70: 4103-4110.   DOI
29 Williams BA, Verstegen MWA, Tamminaga S. 2001. Fermentation in the large intestine of single-stomached animals and its relationship to animal health. Nutr. Res. Rev. 14: 207-227.   DOI