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http://dx.doi.org/10.4014/mbl.1606.06004

Isolation of Potent Amylolytic Fungus Rhizopus oryzae from Nuruk  

Choi, Yeong-Hwan (Kooksoondang Brewery Co. Ltd.)
Choi, Da-Hye (Department of Food Science and Biotechnology, Kangwon National University)
Park, Eun-Hee (Department of Food Science and Biotechnology, Kangwon National University)
Kim, Myoung-Dong (Department of Food Science and Biotechnology, Kangwon National University)
Publication Information
Microbiology and Biotechnology Letters / v.44, no.3, 2016 , pp. 376-382 More about this Journal
Abstract
The amylolytic enzyme activities of nuruks collected or produced in this study were examined. A maximum α-amylase activity of 24,747.1 ± 777.7 units/mg protein was obtained for a nuruk incubated at a relative humidity of 40% at 30℃. A nuruk matured at a relative humidity of 50% at 25℃ showed the highest glucoamylase acitivity. Among the 98 fungal strains isolated from the nuruk exhibiting the highest amylolytic enzyme activities, 26 strains of Aspergillus oryzae and 18 strains of Rhizopus oryzae were identified. Rhizopus oryzae MBF345 showed an α-amylase activity of 36,724.9 ± 10.2 units/mg protein and a glucoamylase activity of 4,911.8 ± 48.1 SP. These values were 1.7-fold and 1.4-fold greater, respectively, than those of the control strain. Strain MBF345 was deposited as KCTC46312 in the Korean Culture Type Collection.
Keywords
Nuruk; α -amylase; glucoamylase; glucoamylase; Rhizopus oryzae;
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1 Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403-410.   DOI
2 Bal J, Yun SH, Song HY, Yeo SH, Kim JH, Kim JM, et al. 2014. Mycoflora dynamics analysis of Korean traditional wheat-based nuruk. J. Microbiol. 52: 1025-1029.   DOI
3 Choi DH, Park EH, Kim MD. 2014. Characterization of starch-utilizing yeast Saccharomycopsis fibuligera isolated from nuruk. Korean J. Microbiol. Biotechnol. 42: 407-412.   DOI
4 Choi JS, Jung ST, Choi JH, Baek SY, Yeo SH. 2012. Quality characteristics of wheat nuruks by storage conditions of liquid starters using Rhizopus oryzae N174. Korean J. Microbiol. Biotechnol. 40: 319-324.   DOI
5 Chung MJ, Hou WN, Jeon JH. 1990. Studies on the development and of three powerful raw starch digesting glucoamylase. J. Agr. Sci. Chungbuk Nat. Univ. 8: 151-163.
6 Huh CK, Kim SM, Kim YD. 2014. Comparison for enzymic activity of nuruk and qualiry properties of Yakju by different fungi. Korean J. Food Preserv. 21: 573-580.   DOI
7 Jang JH. 1991. A history of Korean traditional liquors. Food Sci. Indust. 24: 61-63.
8 Kim HS, Hyun JS, Kim J, Ha HP, Yu TS. 1997. Characteristics of useful fungi isolated from traditional Korean nuruk. J. Korean Soc. Food Sci. Nutr. 26: 767-774.
9 Jo GY, Lee CW. 1997. Isolation and identification of the fungi from nuruk. J. Korean Soc. Food Sci. Nutr. 26: 759-766.
10 Kang HR, Lee AR, Kwon YH, Kim HR, Ahn BH. 2012. Optimization of culture conditions for the yeast and analysis of qualities of Makgeolli brewed with the yeast isolated from Korean traditional nuruk. Korean J. Mycol. 40: 204-209.   DOI
11 Kim CJ, Oh MJ, Lee JS. 1985. Studies on digestion of raw starch by Rhizopus oryzae. Korean J. Appl. Microbiol. Bioeng. 13: 329-337.
12 Kim JH, Kwon YH, Lee AR, Kim HR, Ahn BH. 2012. Manufacture of Koji using fungi isolation from nuruk and identification of Koji molds. Korean J. Mycol. 40: 187-190.   DOI
13 Lee JH, Jwon YJ, Song HG. 2012. Relationships between consumers well-being recognition and Korean traditional liquor selection properties. J. Foodserv. Manage. Soc. Kor. 15: 163-183.
14 Kim JY, Park GS. 2014. Analysis of consumer's present use and future demand of traditional Korean liquors. Korean J. Food Cook Sci. 30: 41-50.   DOI
15 Kim MJ. 2002. The study about traditional nuruk. J. Natural. 9: 291-310.
16 Kim MS, Jeon JA, Jeong ST, Choi JH, Choi HS, Yeo SH. 2011. Characteristics of Byeo-nuruk according to the mixing ratio of wheat and the addition rate of moisture. J. East Asian Soc. Dietary Life 21: 897-904.
17 Lee SH, Beaek SY, Kang JE, Jeon CO, Kin DH, Kim MD, et al. 2015. Effects of temperature on the changes of enzymatic activities and metabolite during wheat nuruk fermentation. Microbiol. Biotechnol. Lett. 43: 378-384.   DOI
18 Nawange SR, Singh SM, Naidu J, Jain S, Nagpal T, Behrani DS, et al. 2012. Zygomycosis caused by Rhizopus microsporus and Rhizopus oryzae in Madhya Pradesh (M.P.) Central India: a report of two cases. Mycopathologia 174: 171-176.   DOI
19 Lee SJ, Ahn BH. 2010. Sensory profiling of rice wines made with nuruks using different ingredients. Korean J. Food Sci. Technol. 42: 119-123.
20 Meussen BJ, de Graaff LH, Sanders JP, Weusthuis RA. 2012. Metabolic engineering of Rhizopus oryzae for the production of platform chemicals. Appl. Microbiol. Biotechnol. 94: 875-886.   DOI
21 Nam K, Lee NK, Yum EJ, Kim YS, Yeo SH, Jeong YS. 2015. Change in the composition and enzyme activity of culturable lactic acid bacteria in nuruk during fermentation at different temperatures. Korean J. Food Preserv. 22: 920-925.   DOI
22 Seo WT, Cho HK, Lee JY, Kim BJ, Cho KM. 2012. Quality characteristics of wheat-rice Makgeolli by making of rice nuruk prepared by Rhizopus oryzae CCS01. Korean J. Microbiol. 48: 147-155.   DOI
23 Park JH, Chung CH. 2014. Characteristics of Takju (a cloudy korean rice wine) prepared with nuruk (a traditional korean rice wine fermentation starter), and identification of lactic acid bacteria in nuruks. Korean J. Food Sci. Technol. 46: 153-164.   DOI
24 Park JW, Kim BJ, Lee JW, Kim YB. 2002. Purification and characterization of a maltopentaose-producing amylase from Bacillus megaterium KSM B-404. Korean J. Microbiol. Biotechnol. 30: 352-358.
25 Pesez M, Bartos J. 1974. Colorimetric and fluorimetric analysis of organic compound and drugs. Marcel Dekker, Inc., New York.
26 Shon SK, Rho YH, Kim HJ, Bae SM. 1990. Takju brewing of uncooked rice starch using Rhizopus koji. Korean J. Appl. Microbiol. Biotech. 18: 506-510.
27 Song SH, Lee C, Lee S, Park JM, Lee HJ, Bai DH, et al. 2013. Analysis of microflora profile in Korean traditional nuruk. J. Microbiol. Biotechnol. 23: 40-46.   DOI
28 Wu G, Nie L, Freeland SJ. 2007. The effects of differential gene expression on coding sequence features: analysis by one-way ANOVA. Biochem. Biophys. Res. Commun. 358: 1108-1113.   DOI