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http://dx.doi.org/10.4014/mbl.1607.07004

Isolation of Stress-tolerant Pichia farinosa from Nuruk  

Kwon, Hun-Joo (Department of Food Science and Biotechnology, Kangwon National University)
Kim, Myoung-Dong (Department of Food Science and Biotechnology, Kangwon National University)
Publication Information
Microbiology and Biotechnology Letters / v.44, no.3, 2016 , pp. 349-354 More about this Journal
Abstract
A variety of nuruks collected in different areas in Korea were explored to isolate sixty yeast strains that was able to grow at 44℃. MBY/L1569 strain, which showed the highest growth rate, was selected and identified as Pichia farinosa (Millerozyma farinosa). The isolated strain exhibited superior resistance to heat, acid, and alkali compared with those of P. farinosa KCTC27412 as a control strain. The specific growth rate of P. farinosa MBY/L1569 at 46℃ was 0.37 ± 0.05 h−1, and the highest specific growth rate of 0.50 ± 0.02 h−1 was obtained when it was grown at pH 7.0 and 37℃ with 50 g/l (w/v) glucose as the carbon source. Under optimum growth conditions, strain MBY/L1569 produced ethanol 19.66 ± 0.68 g/l from glucose 50 g/l, with an approximate yield of 40%. P. farinosa MBY/L1569 was deposited at the Korean Collection for Type Cultures as pichia farinosa KCTC27753.
Keywords
Nuruk; stress tolerance; ethanol; specific growth rate; Pichia farinosa;
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Times Cited By KSCI : 9  (Citation Analysis)
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