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http://dx.doi.org/10.4014/mbl.1605.05001

Growth Enhancement of Shrimp and Reduction of Shrimp Infection by Vibrio parahaemolyticus and White Spot Syndrome Virus with Dietary Administration of Bacillus sp. Mk22  

Sekar, Ashokkumar (Department of Bioscience and Biotechnology, The University of Suwon)
Packyam, Mayavu (CAS in Marine Biology, Annamalai University)
Kim, Keun (Department of Bioscience and Biotechnology, The University of Suwon)
Publication Information
Microbiology and Biotechnology Letters / v.44, no.3, 2016 , pp. 261-267 More about this Journal
Abstract
The present study examined the effect of the dietary administration of a halophilic bacterium Bacillus sp. Mk22 on the growth improvement of black tiger shrimp, Penaeus monodon, and reduced shrimp infection by Vibrio parahaemolyticus and white spot syndrome virus (WSSV). The shrimp were fed 45 days using three experimental diets: no addition (control), commercial probiotic, and Bacillus sp. Mk22. The shrimp treated with the halophilic bacterium Mk22 showed a significant improvement of growth (7.1 ± 0.21 g), survival (94.3 ± 0.58%), weight gain (178 ± 4.93 g), and reduced feed conversion rate (0.8 ± 0.03 g) compared with the shrimp in the other groups. The shrimp treated with Bacillus sp. Mk22 also showed a lower Vibrio count (0.02 ± 0.01 × 102 CFU/ml) in the shrimp culture water compared with the other groups. The shrimp in the three groups were challenged with either Vibrio or WSSV. For the Vibrio infection, no mortality was observed from water infection or oral feeding infection in the commercial probiotic group and Mk 22 group. For the WSSV infection, a 68% survival rate from water infection and 20% survival rate from oral feeding infection was observed on day 45 in the Mk22 group, while 100% mortalities were recorded at a much earlier time in both the control and commercial probiotic groups. The antioxidant enzyme activities, indicators of oxidative stress, such as catalase and superoxide dismutase, significantly decreased in both the Vibrio and WSSV-infected Mk22 groups compared with the other groups, indicating that Bacillus sp. Mk22 was effective in reducing oxidative stress, possibly due to the reduced infection.
Keywords
Bacillus sp. Mk22; Penaeus monodon; reduction of infection; Vibrio parahaemolyticus; white spot syndrome virus; antioxidant enzymes;
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