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http://dx.doi.org/10.4014/mbl.1511.11015

Increase of Epigallocatechin in Green Tea Extract by Lactic Acid Bacteria Fermentation  

Choi, Chan-Yeong (Department of Food Science and Biotechnology, Kangwon National University)
Park, Eun-Hee (Department of Food Science and Biotechnology, Kangwon National University)
Ju, Yoong-Woon (Dadangand Co.)
Kim, Myoung-Dong (Department of Food Science and Biotechnology, Kangwon National University)
Publication Information
Microbiology and Biotechnology Letters / v.44, no.1, 2016 , pp. 62-67 More about this Journal
Abstract
Hydrolytic enzyme activities, including those of ${\beta}$-glucosidase, ${\beta}$-glucuronidase, ${\beta}$-xylosidase, ${\beta}$-galactosidase, ${\beta}$-arabinofuranosidase, ${\beta}$-arabinosidase, and ${\beta}$-arabinopyranosidase, which are useful for bioconversion, were explored in lactic acid bacteria isolated from Korean traditional fermented foods. Nine bacterial strains were selected for the fermentation of green tea extract prepared by supercritical fluid extraction. Changes in the concentrations of catechin, epicatechin, epicatechin gallate, epigallocatechin, and epigallocatechin-3-gallate in green tea extract were investigated after fermentation by the selected lactic acid bacteria strains. The strain Leuconostoc mesenteroides MBE1424, which showed the highest ${\beta}$-glucuronidase enzyme activity among the tested bacterial strains, increased the epigallocatechin content of the green tea extract by 60%. In addition, L. mesenteroides MBE1424 was more resistant than the control strain at high temperature and showed a maximum specific growth rate at $40^{\circ}C$. L. mesenteroides MBE1424 was presumed to have an enzyme system containing ${\beta}$-glucuronidase with utility in the bioconversion of green tea extract.
Keywords
Green tea extract; Leuconostoc mesenteroides; catechin; supercritical fluid extraction; fermented foods;
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1 Ahn JE, Kim JK, Lee HR, Eom HJ, Han NS. 2012. Isolation and characterization of a bacteriocin-producing Lactobacillus sakei B16 from Kimchi. J. Korean Soc. Food Sci. Nutr. 41: 721−726.   DOI
2 Amin HAS, El-Menoufy HA, El-Mehalawy AA, Mostafa ES. 2011. Biosynthesis of glycyrrhetinic acid 3-O-mono-β-D-glucuronide by free and immobilized Aspergillus terreus β-D-glucuronidase. J. Mol. Catal. B. Enzym. 69: 54−59.   DOI
3 Beggs WH, Rogers P. 1966. Galactose repression of β-galactosidase induction in Escherichia coli. J. Bacteriol. 91: 1869−1874.
4 Bursill CA, Abbey M, Roach PD. 2007. A green tea extract lowers plasma cholesterol by inhibiting cholesterol synthesis and upregulating the LDL receptor in the cholesterol-fed rabbit. Atherosclerosis 193: 86−93.   DOI
5 Choi SY, Jung BM, Kim HJ, Seong SH, Kim WJ, Park WS. 2000. Extracellular enzyme activities of the lactic acid bacteria isolated from kimchi. Korean J. Appl. Microbiol. Biotechnol. 28: 59−61.
6 Chung YH, Shin MK. 2005. A study on the physicochemical properties of korean teas according to degree of fermentation. Korean J. Food & Nutr. 18: 944−101.
7 Han SK, Song YS, Lee JS, Bang JK, Suh SJ, Cho JY, et al. 2010. Changes of the chemical constituents and antioxidant activity during microbial-fermented tea (Camellia sinensis L.) processing. Korean J. Food Sci. Technol. 42: 21−26.
8 Elisa T, Maurizio LG, Santo G, Danila DM, Marco G. 2007. Citrus flavonoids: Molecular structure, biological activity and nutritional properties: A review. Food Chem. 104: 466−479.   DOI
9 Gamero A, Manzanares P, Querol A, Belloch C. 2011. Monoterpene alcohols release and bioconversion by Saccharomyces species and hybrids. Int. J. Food Microbiol. 145: 92−97.   DOI
10 Gottschalk TE, Nielsen JE, Rasmussen P. 1996. Detection of endogenous β-glucuronidase activity in Aspergillus niger. Appl. Microbiol. Biotechnol. 45: 240−244.   DOI
11 Jang MH, Kim MD. 2010. Exploration of β-glucosidase activity of lactic acid bacteria isolated from kimchi. Food Eng. Prog. 14: 243−248.
12 Jang MH, Kim MD. 2011. β-1,4-xylosidase activity of Leuconostoc lactic acid bacteria isolated from Kimchi. Korean J. Food Sci. Technol. 43: 169−175.   DOI
13 Jin HS, Kim JB, Tun TJ, Lee KJ. 2008. Selection of Kimchi starters based on the microbial composition of Kimchi and their effects. J. Korean Soc. Food Sci. Nutr. 37: 671−675.   DOI
14 Kim HS, Kim JY, Park MS, Zheng H, Ji GE. 2009. Cloning and expression of β-glucuronidase from Lactobacillus brevis in E. coli and application in the bioconversion of baicalin and wogonoside. J. Microbiol. Biotechnol. 19: 1650−1655.   DOI
15 Kim JI, Row KH. 2001. Recovery of catechin compound from korean green tea by solvent extraction and partition. Korean J. Biotechnol. Bioeng. 16: 442−445.
16 McCleary BV, McKie VA, Draga A, Rooney E, Mangan D, Larkin J. 2015. Hydrolysis of wheat flour arabinoxylan, acid-debranched wheat flour arabinoxylan and arabino-xylo-oligosaccharides by β-xylanase, α-L-arabinofuranosidase and β-xylosidase. Carbohydr. Res. 407: 79−96.   DOI
17 Koo YC, Lee HS, Park BG, Kim EJ, Lee SJ, Kim KH, et al. 2006. Chromosome aberration test of water extract of decaffeined green tea using supercritical carbon dioxide with mannalian cell line. Env. Mutagens Carcinogens 26: 63−68.
18 Lucien FP, Foster NR. 2000. Solubilities of solid mixtures in supercritical carbon dioxide: a review. J. Supercritical Fluids 17: 111−134.   DOI
19 Marcolongo L, Ionata E, La Cara F, Amore A, Giacobbe S, Pepe O, Faraco V. 2014. The effect of pleurotus ostreatus arabinofuranosidase and its evolved variant in lignocellulosic biomasses conversion. Fungal Genet. Biol. 72: 162−167.   DOI
20 Oh JH, Kim EH, Kim JL, Moon YI, Kang YH, Kang JS. 2004. Study on antioxidant potency of green tea by DPPH method. J. Korean Soc. Food Sci. Nutr. 33: 1079−1084.   DOI
21 Park CD, Jung HK, Park CH, Jung YS, Hong JH, Ko HS, et al. 2012. Isolation of citrus peel flavonoid bioconversion microorganism and inhibitory effect on the oxidative damage in pancreatic beta cells. Korean Soc. Biotechnol. Bioeng. J. 27: 67−74.
22 Park CD, Jung HK, Park HH, Hong JH. 2007. Identification and fermentation characteristics of lactic acid bacteria isolated form Hahyangju nuruk. Korean J. Food Preserv. 14: 188−193.
23 Park SB, Han BK, Oh YJ, Lee SJ, Cha SK, Park YS, Choi HJ. 2012. Bioconversion of green tea extract using lactic acid bacteria. Food Eng. Prog. 16: 26−32.
24 Ryu OH, Lee J, Lee KW, Kim HY, Seo JA, Kim SG, et al. 2006. Effects of green tea consumption on inflammation, insulin resistance and pulse wave velocity in type 2 diabetes patients. Diabetes Res. Clin. Pract. 71: 356−358.   DOI
25 Ra YJ, Lee YW, Kim JD, Row KH. 2001. Supericritical fluid extraction of catechin compounds from green tea. Korean J. Biotechnol. Bioeng. 16: 327−331.
26 Rasheed A, Haider M. 1998. Antibacterial activity of Camellia sinensis extracts against dental caries. Arch. Pharm. Res. 21: 348−352.   DOI
27 Shim KS, Park GG, Park YS. 2014. Bioconversion of puffed red ginseng extract using β-glucosidase-producing lactic acid bacteria. Food Eng. Prog. 18: 332−340.   DOI
28 Rhimi M, Aghajari N, Jaouadi B, Juy M, Boudebbouze S, Maguin E, et al. 2009. Exploring the acidotolerance of β-galactosidase from Lactobacillus delbrueckii subsp. bulgaricus: an attractive enzyme for lactose bioconversion. Res. Microbiol. 160: 775−784.   DOI
29 Sá-Nogueira I, Nogueira TV, Soares S, de Lencastre H. 1997. The Bacillus subtilis L-arabinose (ara) operon: nucleotide sequence, genetic organization and expression. Microbiology 143: 957−969.   DOI
30 Sears KD, Casebier RL, Hergert HL. 1974. The structure of catechinic acid. A base rearrangement product of catechin. J. Org. Chem. 39: 3244−3247.   DOI
31 Shin HY, Park SY, Sung JH, Kim DH. 2003. Purification and characterization of α-L-arabinopyranosidase and α-L-arabinofuranosidase from Bifidobacterium breve K-110, a human intestinal anaerobic bacterium metabolizing ginsenoside Rb2 and Rc. Appl. Environ. Microbiol. 69: 7116−7123.   DOI
32 So MH, Lee YS. 1997. Influences of cultural temperature on growth rates of lactic acid bacteria isolated from kimchi. Korean J. Food Nutr. 10: 110−116.
33 Suganuma M, Okabe S, Sueoka N, Sueoka E, Matsuyama S, Imai K, et al. 1999. Green tea and cancer chemoprevention. Mutat. Res. 428: 339−344.   DOI
34 Uesaka E, Sato M, Raiju M, Kaji A. 1978. α-L-Arabinofuranosidase from Rhodotorula flava. J. Bacteriol. 133: 1073−1077.
35 Yang MC, Kim DS, Jeong, SW, Ma JY. 2011. Bioconversion constituents of galgeun-tang fermented by Lactobacillus plantarum. J. Korean J. Medicinal Crop. Sci. 19: 446-455.   DOI
36 Yoo SK, Hur SS, Song SH, Kim KM, Whang KS. 2005. Optimizatin of mannitol fermentation by Leuconostoc mesenteroides sp. strain JFY. J. Life Sci. 15: 374−381.   DOI