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http://dx.doi.org/10.4014/mbl.1511.11014

A Rapid Isolation Method for Bacillus coagulans from Rice Straw  

Lee, Bitnara (Department of Food Science and Biotechnology, Kyonggi University)
Lee, Hyundong (Department of Food Science and Biotechnology, Kyonggi University)
Jeong, Do-Won (Department of Food Science and Biotechnology, Shinansan University)
Lee, Jong-Hoon (Department of Food Science and Biotechnology, Kyonggi University)
Publication Information
Microbiology and Biotechnology Letters / v.43, no.4, 2015 , pp. 401-404 More about this Journal
Abstract
Bacillus coagulans has been considered to be a prominent candidate for probiotics as well as a thermotolerant biomaterial producer. However, the species has not attracted the attention of Korean researchers, nor has it been commercialized in Korea. Therefore, isolates for functional studies are not readily available. To secure B. coagulans resources for future applications, we developed a rapid isolation method for the species from rice straw. Introduction of the enrichment culture at $50^{\circ}C$, the selection of acid producers with $CaCO_3$ supplemented medium, and the elimination of enterococci by selective medium, rendered the successful and rapid isolation of B. coagulans strains.
Keywords
Bacillus coagulans; rapid isolation; rice straw; thermotolerance;
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1 Araya M, Morelli L, Reid G, Sanders ME, Stanton C. 2002. Joint FAO/WHO Working Group Report on Guidelines for the Evaluation of Probiotics in Food. London, Ontario, Canada. ftp://ftp.fao.org/es/esn/food/wgreport2.pdf.
2 Barbosa TM, Serra CR, La Ragione RM, Woodward MJ, Henriques AO. 2005. Screening for Bacillus isolates in the broiler gastrointestinal tract. Appl. Environ. Microbiol. 71: 968-978.   DOI
3 Bergey DH, Breed RS, Murray EGD, Hitchens AP. 1939. Bergey's Manual of Determinative Bacteriology, 5th Ed. The Williams and Wilkins Co., Baltimore, Maryland.
4 Breed RS, Murray EGD, Smith NR. 1957. Bergey's Manual of Determinative Bacteriology, 7th Ed. The Williams and Wilkins Co., Baltimore, Maryland.
5 Cutting SM. 2011. Bacillus probiotics. Food Microbiol. 28: 214-220.   DOI
6 De Clerck E, Rodriguez-Diaz M, Forsyth G, Lebbe L, Logan NA, DeVos P. 2004. Polyphasic characterization of Bacillus coagulans strains, illustrating heterogeneity within this species, and emended description of the species. Syst. Appl. Microbiol. 27: 50-60.   DOI
7 Hajna AA, Perry CA. 1943. Comparative study of presumptive and confirmative media for bacteria of the coliform group and for fecal streptococci. Am. J. Public Health Nations Health 33: 550-556.   DOI
8 Jeong DW, Kim HR, Jung G, Han S, Kim CT, Lee JH. 2014. Bacterial community migration in the ripening of doenjang, a traditional Korean fermented soybean food. J. Microbiol. Biotechnol. 24: 648-660.   DOI
9 Labuda R, Piovarciova Z, Tancinova D. 2008. Sensitivity of the Alternaria infectoria species-group 1 to cycloheximide. Lett. Appl. Microbiol. 46: 673-675.   DOI
10 Lee S-H, Park P. 2010. Isolation of major microflora Bacillus coagulans from rice bran. Korean J. Food Preserve. 17: 165-168.
11 Qin J, Zhao B, Wang X, Wang L, Yu B, Ma Y, et al. 2009. Non-sterilized fermentative production of polymer-grade L-lactic acid by a newly isolated thermophilic strain Bacillus sp. 2-6. PLoS One 4: e4359.   DOI
12 Spinosa MR, Braccini T, Ricca E, De Felice M, Morelli L, Pozzi G, et al. 2000. On the fate of ingested Bacillus spores. Res. Microbiol. 151: 361-368.   DOI
13 Tuohy KM, Pinart-Gilberga M, Jones M, Hoyles L, McCartney AL, Gibson GR. 2007. Survivability of a probiotic Lactobacillus casei in the gastrointestinal tract of healthy human volunteers and its impact on the faecal microflora. J. Appl. Microbiol. 102: 1026-1032.
14 Ye L, Zhou X, Hudari MS, Li Z, Wu JC. 2013. Highly efficient production of L-lactic acid from xylose by newly isolated Bacillus coagulans C106. Bioresour. Technol. 132: 38-44.   DOI
15 Zhang Y, Chen X, Luo J, Qi B, Wan Y. 2014. An efficient process for lactic acid production from wheat straw by a newly isolated Bacillus coagulans strain IPE22. Bioresour. Technol. 158: 396-399.   DOI