Browse > Article
http://dx.doi.org/10.4014/mbl.1507.07005

Characterization of Chlorella vulgaris Mutants Producing High Chlorophyll  

Park, Hyun-Jin (Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University)
Kim, Ok Ju (Chloland Co. Ltd.)
Ha, Ji Min (Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University)
Choi, Tae O (Chloland Co. Ltd.)
Lee, Jae-Hwa (Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University)
Publication Information
Microbiology and Biotechnology Letters / v.43, no.3, 2015 , pp. 275-279 More about this Journal
Abstract
Micro-algae are unicellular photosynthetic organisms and produce pigments such as chlorophyll and carotenoid. Chlorella contains a lot of protein and functional components like lipids, chlorophyll and carotenoids. In this study we induced mutants of Chlorella vulgaris (C. vulgaris) through ultraviolet radiation (UV-B) and selected two mutants by pigment (chlorophyll and carotenoids) content. We named the mutants ‘UBM1-2’, ‘UBM2-57’ and they were cultivated for 21-days. Cell growth, dry cell weight, protein content, lipid and pigments content were measured. The results indicated that the mutants displayed slower cell growth, lower dry cell weight and protein content than the wild type. However, for UBM1-2 the lipid content was 21% higher than the wild type. In addition, the mutants’ chlorophyll content was 37% and 89% higher than the wild type and the carotenoids content was 27% and 70% higher than the wild type, respectively.
Keywords
Microalgae; C. vulgaris; chlorophyll; carotenoids; UV-B;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
연도 인용수 순위
1 Park JI, Woo H-C, Lee J-H. 2008. Production of bio-energy from marine algae: status and perspectives. Korean Chem. Eng. Res. 46: 833−844.
2 Yun HJ, Sung JK, Lee SY, Lee YJ, Ha SK, Sonn YK. 2014. Changes in photosynthesis and carbohydrate synthesis in response to elevated UV-B environment. CNU J. Agricul. Sci. 41: 275−281.   DOI
3 Kim J-H, Park H-J, Kim Y-H, Joo H, Lee S-H, Lee J-H. 2013. UV-induced mutagenesis of Nannochloropsis oculata for the increase of lipid accumulation and its characterization. Appl. Chem. Eng. 24: 155−160.
4 Kim OJ, Lee J-H. 2015. Characterization of Chlorella Vulgaris mutants generated by EMS (Ethyl Methane Sulphonate). Appl. Chem. Eng. 26: 265−269.   DOI
5 Kim YH. 1999. The effect on bioactivities of Chlorella. Food Ind. 10: 122−128.
6 Kim Y-H, Lee J-H. 2012. Isolation of Arthrospira platensis mutants producing high lipid and phycobiliproteins. Korean Soc. Biotecnol. Bioeng. J. 27: 172−176.
7 Lee S-I, Park J-Y, Jung J-C, Lee D-C, Lee S-H, Ha I-M, et al. 2005. Effects of carbon source on production of leucocin a from transformed saccharomyces cerevisiae. J. Life Sci. 15: 847−850.   DOI
8 Marckerness SAH, Butt JP, Jordan BR, Thomas B. 1996. Ameriolation of ultraviolet-B-induced down-regulation of mRNA levels for chloroplast proteins, by high irradiance, is mediated by photosynthesis. J. Plant Physiol. 148: 100−106.   DOI
9 Park B-J, Kim B-M, Shim S-H, Kim J-D, Lee C-G. 2006. Enhancement of astaxanthin production of Haematococcus pluvialis by mutation. Korean J. Microbiol. Biotechnol. 34: 136−142.
10 Park H-J, Jin E-J, Jung T-M, Joo H, Lee J-H. 2010. Optimal culture conditions for photosynthetic microalgae Nannochloropsis oculata. Appl. Chem. Eng. 21: 659−663.
11 Park H-J, Kim Y-H, Lee J-H. 2012. Characterization of Arthrospira platensis mutants generated by UV-B irradiation. Appl. Chem. Eng. 23: 496−500.
12 Park J-H, Han TJ. 1999. Overview of UV-absorbing pigments in marine algae. Algae 14: 201−212.
13 Han JG, Kang GG, Kim JK, Kim SH. 2002. The present status and future of Chlorella. Food Sci. Ind., 6: 64−69.
14 Chen W, Sommerfeld M, Hu Q. 2011. Microwave assisted nile red method for in vivo quantification of neutral lipids in microalgae. Biores. Technol. 102: 135−141.   DOI
15 Choi S-J, Kim Y-H, Kim A, Lee J-H. 2013. Arthrospira platensis mutants containing high lipid content by electron beam irradiation and analysis of its fatty acid composition. Appl. Chem. Eng. 24: 628−632.   DOI
16 Choi S-J, Lee J-H. 2015. Characteristic of Arthrospira platensis enhanced antioxidant activity. Korean Soc. Biotecnol. Bioeng. J. 30: 119−124.
17 Han TJ. 1999. Overview of UV-B effects on marine algae. Korean J. Environ. Biol. 17: 1−9.
18 Illman AM, Scragg AH, Shales SW. 2000. Increase in Chlorella strains calorific values when grown in low nitrogen medium. Enzyme and Microb. Technol. 27: 631−635.   DOI
19 Jeong UC, Han JC, Choi BD, Kang SJ. 2013. Lipid and fatty acid composition in Nannochloropsis oculata cultured in varying salinities. Korean J. Fish Aquat. Sci. 46: 252−258.   DOI
20 Jo BH, Cha HJ. 2010. Biodiesel production using microalgal marine biomass. Korean Soc. Biotecnol. Bioeng. J. 25: 109−115.
21 Kang M-S, Sim S-J, Chae HJ. 2004. Chlorella as a functional biomaterial. Korean Soc. Biotecnol. Bioeng. J. 19: 1−11.
22 Kim DC, Won SI, In MJ. 2014. Preparation and quality characteristics of Mul-kimchi added with chlorella. J. Appl. Biol. Chem. 57: 23−28.   DOI
23 Barclay WR, Meager KM, Abril JR. 1994. Heterotrophic production of long chain omega-3 fatty acids uilizing algae and algae-like microorganisms. J. Appl. Phycol. 6: 123−129.   DOI
24 Abo-Shady AM, Al-ghaffar BA, Rahhal MMH, Abd-El Monem A. 2007. Biological control of faba bean pathogenic fungi by three cyanobacterial filtrates. Pakistan J. Biol. Sci. 10: 3029−3038.   DOI