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http://dx.doi.org/10.4014/mbl.1503.03007

Characteristics of Morphological and Physiological Changes during the Autolysis Process of Saccharomyces cerevisiae FX-2  

Li, Xiao (College of Biological and Pharmaceutical Sciences, China Three Gorges University)
Shi, Xiaodan (College of Biological and Pharmaceutical Sciences, China Three Gorges University)
Zou, Man (College of Biological and Pharmaceutical Sciences, China Three Gorges University)
Luo, Yudi (College of Biological and Pharmaceutical Sciences, China Three Gorges University)
Tan, Yali (Angel Yeast Co., Ltd)
Wu, Yexu (Angel Yeast Co., Ltd)
Chen, Lin (Angel Yeast Co., Ltd)
Li, Pei (Angel Yeast Co., Ltd)
Publication Information
Microbiology and Biotechnology Letters / v.43, no.3, 2015 , pp. 249-258 More about this Journal
Abstract
In this paper, the autolysis process of Saccharomyces cerevisiae FX-2 (S. cerevisiae FX-2) via, a variety of endogenous enzyme, was investigated systematically by analyzing changes in physicochemical parameters in autolysate, surface morphology and the internal structure of the yeast cells. As an explicit conclusion, the arisen autolysis depended on the pH and the optimal pH was found to be 5.5. Based on the experimental data and the characteristics of mycelia morphology, a hypothesis is put forward that simple proteins in yeast vacuolar are firstly degraded for utilization, and then more membrane-bound proteins are hydrolyzed to release hydrolytic enzymes, which arouse an enzymatic reaction to induce the collapse of the cell wall into the cytoplasm.
Keywords
Saccharomyces cerevisiae; autolysis; cellular morphology; scanning electron microscopy; transmission electron microscope;
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