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http://dx.doi.org/10.4014/mbl.1502.02007

Isolation and Characterization of Wild Yeasts for Improving Liquor Flavor and Quality  

Baek, Seong Yeol (Fermented Food Division, Department of Agro-food Resource, NAAS, RDA)
Lee, You Jung (Fermented Food Division, Department of Agro-food Resource, NAAS, RDA)
Kim, Jae Hyun (Fermented Food Division, Department of Agro-food Resource, NAAS, RDA)
Yeo, Soo-Hwan (Fermented Food Division, Department of Agro-food Resource, NAAS, RDA)
Publication Information
Microbiology and Biotechnology Letters / v.43, no.1, 2015 , pp. 56-64 More about this Journal
Abstract
It has been known for some time to the wine industry that non-Saccharomyces yeasts play an important role in increasing volatile components through the secretion of extracellular enzymes. The objective of this study was to investigate what types of enzymes are produced by 1,007 non-Saccharomyces yeast strains isolated from Korean fermented foods. Among 1,007 yeast strains, the 566, 45 and 401 strains displayed β-glucosidase, glucanase and protease activity, respectively. In addition, the 563 and 610 strains possessed tolerances against cerulenin and TFL, and the 307 strain was tolerant to 15% ethanol. Yeasts producing harmful biogenic amines and hydrogen sulfide were excluded from further study, and eventually 12 yeast strains belonging to the genera Wickerhamomyces, Hanseniaspora, Pichia, Saccharomyces were identified, based on the 26S rRNA gene sequences. Among the 12 strains, the 9 and 5 strains possessed glucose and ethanol tolerance, respectively. Yeasts belonging to the genus Saccharomyces produced more than 8% alcohol, but non-Saccharomyces yeasts produced only 3% alcohol.
Keywords
Yeast; non-Saccharomyces; extracellular enzyme; fermentation; liquor;
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1 Viana F, Gil JV, Genovés S, Vallés S, Manzanares P. 2008. Rational selection of non-Saccharomyces wine yeasts for mixed starters based on ester formation and enological traits. Food Microbiol. 25: 778-785.   DOI
2 Yi SH, Kwon SJ, Ahn C, Yoo JY. 1997. Isolation, identification and cultural conditions of yeasts from traditional Meju. Korean J. Appl. Microbiol. Biotechnol. 25: 435-441.
3 Yoshikawa K. 1999. Sake: production and flavor. Food Rev. Int. 15: 83-107.   DOI
4 Nikolaoua E, Soufleros EH, Bouloumpasi E, Tzanetakis N. 2006. Selection of indigenous Saccharomyces cerevisiae strains according to their oenological characteristics and vinification results. Food Microbiol. 23: 205-211.   DOI
5 Shin KR, Kim BC, Yang JY, Kim YD. 1999. Characterization of Yakju prepared with yeasts from fruits. 1. Volatile components in Yakju during fermentation. J. Korean Soc. Food Sci. Nutr. 28: 794-800.
6 Oba T, Nomiyana S, Hirakawa H, Tashiro K, Kuhara S. 2005. Asp578 in Leu4p is one of the key residues for leucine feedback inhibition release in sake yeast. Biosci. Biotechnol. Biochem. 69: 1270-1273.   DOI
7 Saitou N, Nei M. 1987. The neighbor joining-methods: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425.
8 Seo MY, Lee JK, Ahn BH, Cha SK. 2005. The changes of microflora during the fermentation of Takju and Yakju. Korean J. Food Sci. Technol. 37: 61-66.
9 Strauss MLA, Jolly NP, Lambrechts MG, Van Rensburg P. 2001. Screening for the production of extracellular hydrolytic enzymes by non-Saccharomyces wine yeasts. J. Appl. Microbiol. 91: 182-190.   DOI
10 Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24: 1596-1599.   DOI
11 Verstrepen KJ, van Laere SDM, Vanderhaegen BMP, Derdelinckx G, Dufour JP, Pretorius IS, et al. 2003. Expression levels of the yeast alcohol acetyltransferase genes ATF1, Lg-ATF1, and ATF2 control the formation of a broad range of volatile esters. Appl. Environ. Microbiol. 69: 5228-5237.   DOI
12 Jung HK, Park CD, Park HH, Lee GD, Lee IS, Hong JH. 2006. Manufacturing and characteristics of Korean traditional liquor, Hahyangju prepared by Saccharomyces cerevisiae HA3 isolated from traditional Nuruk. Korean J. Food Sci. Technol. 38:659-667.
13 Kwon SJ, Ahn TY, Sohn JH. 2012. Analysis of microbial diversity in Makgeolli fermentation using PCR-DGGE. J. Life Sci. 22: 232-238.   DOI   ScienceOn
14 Lee HS, Lee TS, Noh BS. 2007. Volatile flavor components in the mashes of Takju prepared using different yeasts. Korean J. Food Sci. Technol. 39: 593-599 .
15 Kaosowski G, Czuprynski B. 2006. Kinetics of acetals and esters formation during alcoholic fermentation of various starchy raw materials with application of yeasts Saccharomyces cerevisiae. J. Food Eng. 72: 242-246.   DOI
16 Kim HR, Kim JH, Bai DH, Ahn BH. 2012. Feasibility of brewing Makgeolli using Pichia anomala Y197-13, a Non-Saccharomyces cerevisiae. J. Microbiol. Biotechnol. 22: 1749-1757.   DOI   ScienceOn
17 Kim MJ, Kim BH, Han JK, Lee SY, Kim KS. 2011. Analysis of quality properties and fermentative microbial profiles of Takju and Yakju brewed with or without steaming process. J. Fd Hyg. Safety 26: 64-69.
18 Lee HS, Park CS, Choi JY. 2010. Quality characteristics of the mashes of Takju prepared using different yeasts. Korean J.Food Sci. Technol. 42: 56-62.
19 Lee MN. 2010. Isolation and characteristics of urease production yeasts from Korean traditional Nuruk. MS thesis, Kyungpook National University, Korea, pp. 5-6.
20 Lee YJ, Choi YR, Lee SY, Park JT, Shim JH, Park KH, et al. 2011. Screening wild yeast strains for alcohol fermentation from various fruits. Korean Soc. Mycol. 39: 33-39.
21 Charoenchai C, Fleet GH, Henschke PA, Todd BEN. 1997. Screening of non-Saccharomyces wine yeasts for the presence of extracellular hydrolytic enzymes. Australian J. GrapeWine Res. 3: 2-8.
22 Ciani M, Beco L, Comitini F. 2006. Fermentation behavior and metabolic interactions of multistarter wine yeast fermentations. Int. J. Food Microbiol. 108: 239-245.   DOI
23 Ichikawa E, Hosokawa N, Hata Y, Abe Y, Suginami K, Imayasu S. 1991. Breeding of sake yeast with improved ethyl caproate productivity. Agric. Biol. Chem. 55: 2153-2154.   DOI
24 Comitini F, Gobbi M, Domizio P, Romani D, Lencioni L, Mannazzu I, et al. 2011. Selected non-Saccharomyces wineyeasts in controlled multistarter fermentations with Saccharomyces cerevisiae. Food Microbiol. 28: 873-882.   DOI   ScienceOn
25 Fleet GH. 1993. The microorganisms of winemaking-isolation, enumeration and identification. In: Fleet GH (ed) Wine microbiology and biotechnology. Harwood Academic Publishers, Switzerland. pp. 1-25.
26 Hernández LF, Espinosa JC, Ferna´ndez-González M, Briones A. 2003. β-glucosidase activity in a Saccharomyces cerevisiae wine strain. Int. J. Food Microbiol. 80: 171-176.   DOI
27 Bussey H, Umbarger HE. 1970. Biosynthesis of branched-chain amino acids in yeast: a trifluoroleucine-resistant mutant with altered regulation of leucine uptake. J. Bacteriol. 103:286-294.
28 Casalone E, Fia G, Barberio C, Cavalieri D, Turbanti L, Polsinelli M. 1997. Genetic and biochemical characterization of Saccharomyces cerevisiae mutants resistant to trifluoroleucine. Res. Microbiol. 148: 613-623.   DOI