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http://dx.doi.org/10.4014/mbl.1412.12001

The Anti-inflammatory Effect of Skipjack Tuna (Katsuwonus pelamis) Oil in LPS-induced RAW 264.7 Cells and Mouse Models  

Kang, Bo-Kyeong (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kim, Min-Ji (Institute of Fisheries Sciences, Pukyong National University)
Kim, Koth-Bong-Woo-Ri (Institute of Fisheries Sciences, Pukyong National University)
Ahn, Na-Kyung (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Choi, Yeon-Uk (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Bark, Si-Woo (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Pak, Won-Min (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kim, Bo-Ram (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Park, Ji-Hye (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Bae, Nan-Young (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Ahn, Dong-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Publication Information
Microbiology and Biotechnology Letters / v.43, no.1, 2015 , pp. 45-55 More about this Journal
Abstract
This study was carried out to demonstrate the anti-inflammatory effect of tuna oil (TO) using LPS-induced inflammation responses and mouse models. First, nitric oxide (NO) and pro-inflammatory cytokines levels were suppressed up to 50% with increasing concentrations of TO without causing any cytotoxicity. Also, the expression of a variety of proteins, such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and nuclear factor kappa B (NF-κB), was suppressed in a dosedependent manner by treatment with TO. Furthermore, TO also inhibited the phosphorylation of mitogen-activated protein kinases (MAPKs), including c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), and p38 protein kinase (p38). Moreover, in in vivo testing the formation of ear edema was reduced at the highest dose tested compared to that in the control, and a reduction of ear thickness and the number of mast cells was observed in histological analysis. In acute toxicity test, no mortalities occurred in mice administrated 5,000 mg/kg body weight of TO over a two-week observation period. Our results suggest that TO has a considerable anti-inflammatory property through the suppression of inflammatory mediator productions and that it could prove to be useful as a potential anti-inflammatory therapeutic material.
Keywords
Tuna oil; anti-inflammatory effect; ear edema; cytokine;
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Times Cited By KSCI : 8  (Citation Analysis)
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