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http://dx.doi.org/10.4014/mbl.1407.07007

A Facile Pretreatment Method for Rice Straw using Electron Beam Irradiation and 4-methylmorpholine-N-oxide Solution  

Lee, Byoung-Min (Research Division for Industry & Environment, Korea Atomic Energy Research Institute)
Lee, Jin-Young (Research Division for Industry & Environment, Korea Atomic Energy Research Institute)
Kang, Phil-Hyun (Research Division for Industry & Environment, Korea Atomic Energy Research Institute)
Jeun, Joon-Pyo (Research Division for Industry & Environment, Korea Atomic Energy Research Institute)
Publication Information
Microbiology and Biotechnology Letters / v.43, no.1, 2015 , pp. 16-21 More about this Journal
Abstract
In this study, a facile two-step pretreatment method was investigated for producing fermentable sugars. Rice straw was pretreated using electron beam irradiation (EBI) and 4-methylmorpholine-N-oxide (NMMO) prior to enzymatic hydrolysis. In the first stage, the EBI on the rice straw was carried out at various doses (100, 300, 500 kGy) and then, irradiated rice straw was stirred with NMMO solution at 120°C for 1 h for the second stage. The pretreated rice straw was hydrolyzed by cellulase 1.5 L (70 FPU/ml) and Novozyme-188 (40 CbU/ml) at 50°C for 24, 48, and 72 h. A sugar yield of 83.8% was obtained from the pretreated rice straw after 72 h of enzymatic hydrolysis. Also, FTIR and XRD results indicate that the pretreatment of the rice straw was effective due to the synergic effects of the two-step pretreatment. In conclusion, rice straw might be a potential substrate for bioethanol production by yeast fermentation.
Keywords
Biomass; pretreatment; lignocellulose; electron beam; rice straw;
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Times Cited By KSCI : 1  (Citation Analysis)
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