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http://dx.doi.org/10.4014/kjmb.1404.04006

Anti-Oxidative and Anti-Inflammatory Effects of Malus huphensis, Ophiorrhiza cantonensis, and Psychotria rubra Ethanol Extracts  

Jin, Kyong-Suk (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
Kwon, Hyun Ju (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
Kim, Byung Woo (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
Publication Information
Microbiology and Biotechnology Letters / v.42, no.3, 2014 , pp. 275-284 More about this Journal
Abstract
This study was orchestrated with the purpose of uncovering new nutraceutical resources possessing biological activities in the plant kingdom. To fulfill our objective, we analyzed several Chinese plants and selected three possessing powerful anti-oxidative activities. The anti-oxidative and anti-inflammatory effects these three Chinese plants, Malus hupehensis, Ophiorrhiza cantonensis, and Psychotria rubra ethanol extracts were then evaluated. First of all, they possessed potent scavenging activity against 1,1-diphenyl-2-picryl hydrazyl, similar with that of ascorbic acid, used as a positive control. Moreover, they inhibited lipopolysaccharide (LPS)- and hydrogen peroxide-induced reactive oxygen species, in a dose-dependent manner, in RAW 264.7 cells. Also, they induced the expression of an anti-oxidative enzyme, heme oxygenase 1, and its upstream transcription factor, nuclear factor-E2-related factor 2. Furthermore, they suppressed LPS-induced nitric oxide (NO) formation, without cytotoxicity. The inhibition of NO formation was the result of the down regulation of inducible NO synthase (iNOS). The suppression of NO and iNOS by the three extracts might be the result of modulation by the upstream transcription factors, nuclear factor ${\kappa}B$ and activator protein-1. Taken together, these results indicate that these three Chinese plants possess potent anti-oxidative and anti-inflammatory activities. Therefore, they might be utilized as promising materials in the field of nutraceuticals.
Keywords
Three Chinese plants; anti-oxidative activity; anti-inflammatory activity; upstream transcription factors;
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1 Li L, Dong H, Song E, Xu X, Liu L, Song Y. 2014. Nrf2/ARE pathway activation, HO-1 and NQO1 induction by polychlorinated biphenyl quinone is associated with reactive oxygen species and PI3K/AKT signaling. Chem. Biol. Interact. 209: 56-67.   DOI
2 Liochev SI. 2013. Reactive oxygen species and the free radical theory of aging. Free Radic. Biol. Med. 60: 1-4.   DOI   ScienceOn
3 Lu Y, Suh SJ, Kwak CH, Kwon KM, Seo CS, Li Y, et al. 2012. Saucerneol F, a new lignan, inhibits iNOS expression via MAPKs, NF-kappaB and AP-1 inactivation in LPS-induced RAW264.7 cells. Int. Immunopharmacol. 12: 175-181.   DOI
4 Noworyta-Sokolowska K, Gorska A, Golembiowska K. 2013. LPS-induced oxidative stress and inflammatory reaction in the rat striatum. Pharmacol. Rep. 65: 863-869.   DOI
5 Park CM, Park JY, Noh KH, Shin JH, Song YS. 2011. Taraxacum officinale Weber extracts inhibit LPS-induced oxidative stress and nitric oxide production via the NF-kappaB modulation in RAW 264.7 cells. J. Ethnopharmacol. 133: 834-842.   DOI   ScienceOn
6 Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB. 2010. Oxidative stress, inflammation, and cancer: how are they linked? Free Radic. Biol. Med. 49: 1603-1616.   DOI   ScienceOn
7 Tsai HH, Lee WR, Wang PH, Cheng KT, Chen YC, Shen SC. 2013. Propionibacterium acnes-induced iNOS and COX-2 protein expression via ROS-dependent NF-kappaB and AP-1 activation in macrophages. J. Dermatol. Sci. 69: 122-131.   DOI   ScienceOn
8 Rosenwasser RF, Sultan S, Sutton D, Choksi R, Epstein BJ. 2013. SGLT-2 inhibitors and their potential in the treatment of diabetes. Diabetes Metab. Syndr. Obes. 6: 453-467.
9 Saw CL, Wu Q, Su ZY, Wang H, Yang Y, Xu X, et al. 2013. Effects of natural phytochemicals in Angelica sinensis (Danggui) on Nrf2-mediated gene expression of phase II drug metabolizing enzymes and anti-inflammation. Biopharm. Drug Dispos. 34: 303-311.   DOI   ScienceOn
10 Su ZY, Shu L, Khor TO, Lee JH, Fuentes F, Kong AN. 2013. A perspective on dietary phytochemicals and cancer chemoprevention: oxidative stress, nrf2, and epigenomics. Top Curr. Chem. 329: 133-162.
11 Awad N, Khatib N, Ginsberg Y, Weiner Z, Maravi N, Thaler I, et al. 2011. N-acetyl-cysteine (NAC) attenuates LPS-induced maternal and amniotic fluid oxidative stress and inflammatory responses in the preterm gestation. Am. J. Obstet. Gynecol. 204: 450.e15-20.   DOI
12 Bryan HK, Olayanju A, Goldring CE, Park BK. 2013. The Nrf2 cell defence pathway: Keap1-dependent and -independent mechanisms of regulation. Biochem. Pharmacol. 85: 705-717.   DOI   ScienceOn
13 Chapple SJ, Siow RC, Mann GE. 2012. Crosstalk between Nrf2 and the proteasome: therapeutic potential of Nrf2 inducers in vascular disease and aging. Int. J. Biochem. Cell Biol. 44: 1315-1320.   DOI   ScienceOn
14 Grivennikov SI, Greten FR, Karin M. 2010. Immunity, inflammation, and cancer. Cell 140: 883-899.   DOI   ScienceOn
15 Chawla A, Nguyen KD, Goh YP. 2011. Macrophage-mediated inflammation in metabolic disease. Nat. Rev. Immunol. 11: 738-749.   DOI   ScienceOn
16 Giudice A, Arra C, Turco MC. 2010. Review of molecular mechanisms involved in the activation of the Nrf2-ARE signaling pathway by chemopreventive agents. Meth. Mol. Biol. 647: 37-74.   DOI   ScienceOn
17 Gonzalez-Burgos E, Gomez-Serranillos MP. 2012. Terpene compounds in nature: a review of their potential antioxidant activity. Curr. Med. Chem. 19: 5319-5341.   DOI
18 Guo DY, Li J, Shi YQ, Wang JZ. 2011. Study on the flavon ingredients of Malus hupehensis. Zhong Yao Cai. 34: 1026-1029.
19 Wang FW, Wang Z, Zhang YM, Du ZX, Zhang XL, Liu Q, et al. 2013. Protective effect of melatonin on bone marrow mesenchymal stem cells against hydrogen peroxide-induced apoptosis in vitro. J. Cell Biochem. 114: 2346-2355.   DOI   ScienceOn
20 Wang SQ, Zhu XF, Wang XN, Shen T, Xiang F, Lou HX. 2013. Flavonoids from Malus hupehensis and their cardioprotective effects against doxorubicin-induced toxicity in H9c2 cells. Phytochemistry 87: 119-125.   DOI
21 Yagi H, Tan J, Tuan RS. 2013. Polyphenols suppress hydrogen peroxide-induced oxidative stress in human bone-marrow derived mesenchymal stem cells. J. Cell Biochem. 114: 1163-1173.   DOI   ScienceOn
22 You-Qin D, Tian-Yan F, Gai-Gai D, Ying L, Jun-Zhi W. 2011. [Inhibitory effect of total flavonoids of Malus hupehensis on hepatic fibrosis induced by Schistosoma japonicum in mice]. Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 23: 551-554.
23 Zhang M, An C, Gao Y, Leak RK, Chen J, Zhang F. 2013. Emerging roles of Nrf2 and phase II antioxidant enzymes in neuroprotection. Prog. Neurobiol. 100: 30-47.   DOI   ScienceOn
24 Zhang R, Kang KA, Piao MJ, Maeng YH, Lee KH, Chang WY, et al. 2009. Cellular protection of morin against the oxidative stress induced by hydrogen peroxide. Chem. Biol. Interact. 177: 21-27.   DOI   ScienceOn
25 Zhao CR, Gao ZH, Qu XJ. 2010. Nrf2-ARE signaling pathway and natural products for cancer chemoprevention. Cancer. Epidemiol. 34: 523-533.   DOI   ScienceOn
26 Kalyanaraman B. 2013. Teaching the basics of redox biology to medical and graduate students: Oxidants, antioxidants and disease mechanisms. Redox. Biol. 1: 244-257.   DOI   ScienceOn
27 Harman D. 2009. Origin and evolution of the free radical theory of aging: a brief personal history, 1954-2009. Biogerontology 10: 773-781.   DOI
28 Hayashi T, Smith FT, Lee KH. 1987. Antitumor agents. 89. Psychorubrin, a new cytotoxic naphthoquinone from Psychotria rubra and its structure-activity relationships. J. Med. Chem. 30: 2005-2008.   DOI
29 Hu R, Saw CL, Yu R, Kong AN. 2010. Regulation of NF-E2-related factor 2 signaling for cancer chemoprevention: antioxidant coupled with antiinflammatory. Antioxid. Redox. Signal. 13: 1679-1698.   DOI   ScienceOn
30 Kedare SB, Singh RP. 2011. Genesis and development of DPPH method of antioxidant assay. J. Food Sci. Technol. 48: 412-422.   DOI   ScienceOn
31 Khansari N, Shakiba Y, Mahmoudi M. 2009. Chronic inflammation and oxidative stress as a major cause of age-related diseases and cancer. Recent. Pat. Inflamm. Allergy Drug. Discov. 3: 73-80.   DOI   ScienceOn
32 Li J, Zhang H, Huang W, Qian H, Li Y. 2012. TNF-alpha inhibitors with anti-oxidative stress activity from natural products. Curr. Top Med. Chem. 12: 1408-1421.   DOI
33 Kundu JK, Surh YJ. 2008. Inflammation: gearing the journey to cancer. Mutat. Res. 659: 15-30.   DOI   ScienceOn
34 Kundu JK, Surh YJ. 2012. Emerging avenues linking inflammation and cancer. Free Radic. Biol. Med. 52: 2013-2037.   DOI   ScienceOn
35 Lee JC, Hou MF, Huang HW, Chang FR, Yeh CC, Tang JY, et al. 2013. Marine algal natural products with anti-oxidative, anti-inflammatory, and anti-cancer properties. Cancer Cell Int. 13: 55.   DOI   ScienceOn