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http://dx.doi.org/10.4014/kjmb.1406.06001

Screening of Bifidobacteria for the Development of Probiotics Inhibiting Intestinal Pathogenic Bacteria  

Lee, Jaeyeon (Institute of Bioindustry, The University of Suwon)
Shin, Yungoh (Institute of Bioindustry, The University of Suwon)
Kim, Keun (Institute of Bioindustry, The University of Suwon)
Publication Information
Microbiology and Biotechnology Letters / v.42, no.3, 2014 , pp. 211-218 More about this Journal
Abstract
In order to isolate probiotic lactic acid bacteria possessing high inhibitory activities against porcine and zoonotic pathogens, such as enterotoxigenic E. coli, Salmonella Typhimurium, and Clostridium perfringens, a total of 65 anaerobic strains were initially isolated from a variety of sources including cattle rumen fluids, chicken intestines and swine feces. Four Bifidobacterium strains were selected for their high anti-pathogenic bacterial activities. By using the 16S rDNA sequencing method, three B. boum strains and one B. thermophilum were identified. B. thermophilum demonstrated the best adhesive ability to epithelial cells of swine intestine among the isolates. Indeed, B. thermophilum was seen to have superior characteristics as a probiotic for swine, as judged by their high growth inhibitory activities against various pathogens, and high acid- and bile-tolerance.
Keywords
Lactic acid bacteria; probiotics; swine; intestinal pathogenic bacteria;
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1 Carghill CF 1982. Control of E. coli infections in pigs. Austral. Adv. Vet. Sci. pp. 206-207.
2 Gueimonde M, Salminen S. 2006. New methods for selecting and evaluating probiotics. Dig. Liver Dis. 38: S242-247.   DOI
3 FAO/WHO. 2001. Health and nutritional properties of probiotics in food, including powder milk with live lactic acid bacteria. Report of a joint FAO/WHO expert consulation on evaluation of health and nutritional properties of probiotics in food, including powder milk with live lactic acid bacteria. FAO, Cordoba, Argentina.
4 Fontana L, Bermudez-Brito M, Plaza-Diaz J, Munoz-Quezada S, Gil A. 2013. Sources, isolation, characterization and evaluation of probiotics. British J. Nutrition. 109: S35-S50.   DOI
5 Fuller R, Gibson GR. 1997. Modification of the intestinal microflora using probiotics and prebiotics. Scand. J. Gastroenterol. 222: 28-31.
6 Hartemink, R, Kok BJ, Weenk GH, Rombouts FM. 1996. Raffinose-Bifidobacterium (RB) agar, a new selective medium for bifidobacteria. J. Microbiol. Methods. 27: 33-43.   DOI   ScienceOn
7 Havenaar R, Brink BT, Veid JHJI. 1992. Selection of strains for probiotic use, pp. 209-224. In Fuller R (ed.), Probiotics : The scientific basis. Chapmann & Hall, London.
8 Hudault, S, Lievin V, Bernet-Camard MF, Servin AL. 1997. Antagonistic activity exerted in vitro and in vivo by Lactobacillus casei (strain GG) against Salmonella Typhimurium C5 infection. Appl. Environ. Microbiol. 63: 513-518.
9 Jonsson E, Conway P. 1992. Probiotic for pigs, pp. 209-224. In Fuller R. (ed.), Probiotics : The scientific basis. Chapmann & Hall, London.
10 Lahteinen, T, Malinen E, Koort JMK, Mertaniemi-Hannus U, Hankimo T, Karikoski N, et al. 2010. Probiotic properties of Lactobacillus isolates originating from porcine intestine and feces. Anaerobe. 16: 293-300.   DOI   ScienceOn
11 Lee DY, Seo YS, Rayamajhi N, Kang ML, Lee SI, Yoo HS. 2009. Isolation, characterization, an evaluation of wild isolates of Lactobacillus reuteri from pig feces. J. Microbiol. 47: 663-672.   DOI
12 Nissen L, Chingwaru W, Sgorbati B, Biavati B, Cencic A. 2009. Gut health promoting activity of new putative probiotic/protective Lactobacillus spp.strains: A functional study in the small intestinal cell model. Int. J. Food Microbiol. 135: 288-294.   DOI
13 Lee JY, Hwang KY, Kim HS, Kim K. 2002. Isolation and identification of lactic acid bacteria inhibiting gastro-intestinal pathogenic bacteria of domestic animal. Korean J. Microbiol. Biotechnol. 30: 129-134.
14 Lee JY, Hwang KY, Kim K, Park YS, Paik MJ, Kim KR. 2002. Characteristics of antimicrobial organic acids produced by Lactobacillus pentosus K34 isolated from small intestines of Korean native chickens. Korean J. Microbiol. Biotechnol. 30: 241-246.
15 Minocha, A. 2009. Probiotics for preventive health. Nutr. Clin. Pract. 24: 227-241.   DOI
16 Nousianen J, Javanainen P, Setala J, von Wright A. 2004. Lactic acid bacteria as animal probiotics, pp. 547-580. In Salminen S, von Wright A, Quwehand A. (eds.) Lactic acid bacteria. Microbiological and functional aspects. 3rd Ed. Marcel Dekker. New York.
17 Petinaki E, Spiliopoulou I. 2012. Methicillin-resistant Staphylococcus aureus among companion and food-chain animals: impact of human contacts. Clin. Microbiol. Infect. 18: 626-634.   DOI
18 Pletinckx LJ, Verhegghe M, Crombe F, Dewulf J, De Bleecker Y, Rasschaert G, et al. 2013. Evidence of possible methicillin-resistant Staphylococcus aureus ST398 spread between pigs and other animals and people residing on the same farm. Prev. Vet. Med. 109: 293-303.   DOI
19 Rodriguez E, Arques JL, Rodriguez R, Nunez M, Medina M. 2003. Reuterin production by lactobacilli isolated from pig faeces and evaluation of probiotic traits. Lett. Appl. Microbiol. 37: 259-263.   DOI   ScienceOn
20 Sanders ME, Kondo JK, Willrett DL. 1991. Application of lactic acid bacteria, pp. 433-459. In Goldberg I. Williams R (eds.), Biotechnology and Food Ingredient, Van Nostrand Reinhold, New York.
21 Taylor DJ. 1999. Clostridial infections, Sec. 3, Bacterial diseases, pp. 395-412. In Taylor DJ (ed.), Diseases of swine, 8th Ed. Iowa State University Press, Ames, Iowa.
22 Wang JQ, Yin FG, Zhu C, Yu H, Niven SJ, deLange CFM, et al. 2012. Evaluation of probiotic bacteria for their effects on the growth performance and intestinal microbiota of newly-weaned pigs fed fermented high-moisture maize. Livestock Sci. 145: 79-86.   DOI
23 Schillinger U, Lucke F. 1989. Antibacterial activity of Lactobacillus sake isolated from meat. Appl. Environ. Microbiol. 55: 1901-1906.
24 Schwartz KJ. 1999. Salmonellosis, Sec. 3, Bacterial diseases, pp.535-551. In Taylor DJ (ed.), Diseases of swine, 8th Ed. Iowa State University Press, Ames, Iowa.
25 Smith HW. 1965. The development of the flora of the alimentary tract in young animals. J. Pathol. Bacteriol. 90: 495-513.   DOI
26 Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, et al. 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res. 25: 3389-3402.   DOI   ScienceOn
27 Backstrom, L. 1973. Environment and health in piglet production. A field study of incidences and litter number on behaviour and physiological responses related to welfare status of individual and group housed pigs. Appl. Anim. Behaviour Sci. 17: 229-243.
28 Adams MR, Nicholaides L. 1997. Reviews of the sensitivity of different foodborne pathogens to fermentation. Food Control. 8: 227-239.   DOI   ScienceOn
29 Alexander TJL. 1994. Neonatal diarrhea in pigs, pp. 151-170. In Gyles CL (ed.), Escherichia coli in Domestic Animals and Humans, CAB international, Wallingford. U.K.
30 Bertschinger HU, Fairbrother JM. 1999. Escherchia coli infections, Sec. 3, Bacterial diseases, pp. 431-468. In Taylor DJ (ed.), Diseases of swine, 8th Ed. Iowa State University Press, Ames, Iowa.
31 Beutin L, Geiser D, Steinruck H, Zimmermann S, Scheutz F. 1998. Prevalence and some properties of verotoxin (shiga-like toxin)-producing E. coli in seven different species of healthy domestic animals. J. Clin. Microbiol. 31: 2483-2488.
32 Bjork AKK, Christensson E, Olsson NG, Martinsson KB. 1984. The clinical effect of amperozide in pig production. I. Effect of amperozide on aggression and performance in weaners. XV. p. 335. Proc. Int. Vet. Soc. 8th Congress, Ghent. Belgium, 27-31 August, 1984.
33 Chang YH, Kim JK, Kim HJ, Kim WY, Kim YB, Park YH. 2001. Selection of a potential probiotic Lactobacillus strain and subsequent in vivo studies. Antonie van Leeuwenhoek 80: 193-199.   DOI   ScienceOn
34 Chou LS, Weimer B. 1999. Isolation and characterization of acid- and bile-tolerant isolates from strains of Lactobacillus acidophilus. J. Dairy Sci. 82: 23-31.   DOI   ScienceOn
35 Coconnier M, Lievin HV, Lorrot M, Servin AL. 2000. Antagonistic activity of Lactobacillus acidophilus LB against intracellular Salmonella enterica serovar Typhimurium infecting human enterocyte-like Caco-2/TC-7 cells. Appl. Environ. Microbiol. 66: 1152-1157.   DOI   ScienceOn
36 De Angelis M, Siragusa S, Berioco M, Caputo L, Settanni L, Alfonsi G. 2006. Selection of potential probiotic lactobacilli from pig feces to be used as additives in pelleted feeding. Res. Microbiol. 157: 792-801.   DOI
37 Dicks LM, Botes M. 2010. Probiotic lactic acid bacteria in the gastro-intestinal tract: health benefits, safety, and mode of action. Benef. Microbes 1: 11-19.   DOI
38 Dunne C. 2011. Adaptation of bacteria to the intestinal niche: Probiotics and gut disorder. Inflam. Bowel Dis. 7: 136-145.
39 Fahy VA, Connaughton D, Driesen SJ, Spicer EM. 1987. Post-weaning colibacillosis, pp. 189-201. In APSA Committee (eds.), Manipulating Pig Production, Australian Pig Science Association, Werribee, Victoria, Australia.