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Isolation and Enzyme Production of a Neutral Protease-Producing Strain, Bacillus sp. DS-1.  

전대식 (배재대학교 생명과학부)
강대경 (이지바이오시스템 생물자원연구소)
김하근 (배재대학교 생명과학부)
Publication Information
Microbiology and Biotechnology Letters / v.30, no.4, 2002 , pp. 346-351 More about this Journal
Abstract
A bacterium producing the neutral pretense was isolated from soil, and was identified as Bacillus sp. DS-1 by 16S rRNA sequence comparison and biochemical determinations. The production of protease from Bacillus sp. DS-1 was increased 20% and 30% by the additions of 1% glucose and 1% yeast extract, respectively. The optimum pH and temperature for the protease activity were pH 7.0 and 55$^{\circ}C$. Bacillus sp. DS-1 produced a metalloprotease as a major protease in culture medium, since the pretense activity in culture supernatant was inhibited by the presence of 1 mM EDTA significantly.
Keywords
Bacillus; 165 rRNA; neutral protease production; EDTA inhibition;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By SCOPUS : 5
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