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http://dx.doi.org/10.7845/kjm.2014.2054

Characteristics of Biosurfactant Producing Pseudomonas sp. HN37  

Jung, Da Hee (Department of Biological Science and Biotechnology, Hannam University)
Chang, Dong Ho (Department of Biological Science and Biotechnology, Hannam University)
Kim, Yeong Eun (Department of Biological Science and Biotechnology, Hannam University)
Jeong, Mi Rang (Department of Biological Science and Biotechnology, Hannam University)
Hahn, Kyu Woong (Department of Biological Science and Biotechnology, Hannam University)
Kim, Hyong Bai (Department of Biotechnology and Bioinformatics, Korea University)
Park, Kyeong Ryang (Department of Biological Science and Biotechnology, Hannam University)
Publication Information
Korean Journal of Microbiology / v.50, no.1, 2014 , pp. 33-39 More about this Journal
Abstract
One hundred forty four bacterial colonies which were able to degrade crude oil were isolated from soil samples that were contaminated with oil in Daejeon area. Among them, one bacterial strain was selected for this study based on its emulsification activity, growth rate and surface tension activity, and this selected bacterial strain was identified as Pseudomonas sp. HN37 through physiological- biochemical tests and analysis of its 16S rRNA sequence. Pseudomonas sp. HN37 utilize the several aliphatic hydrocarbons, 3,5-dinitrosalicylic acid and 2,4-dichlorophooxyacetic acid as a sole carbon source. And this bacterial strain showed a high resistance to antibiotics such as ampicillin and chloramphenicol, as well as heavy metals such as Ba, Cr, Li and Mn. Also, it was found that the optimal pH and temperature for the cell growth, surface tension, and emulsification activity of Pseudomonas sp. HN37 were pH 6.0-9.0 and $30^{\circ}C$, respectively. The emulsification and surface tension activity was reached the maximum to 1% (V/V) crude oil and 1% (W/V) NaCl concentration. The surface tension of the culture broth was decreased from 62 to 27 dyne/cm after fifteen hours of inoculation in LB media.
Keywords
Pseudomonas sp. HN37; biosurfactant; colony count; emulsification; surface tension;
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