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http://dx.doi.org/10.5657/KFAS.2021.0912

Detection Characteristics and Antimicrobial Resistance of Vibrio vulnificus Isolated from Sea Water Along the Gadeok Island Coast  

Oh, Hee-Kyung (Department of Industry 4.0 Convergence Bionics Engineering, Pukyong National University)
Jeong, Hee-Jin (Department of Food Science and Technology, Pukyong National University)
Jeong, Geum-Jae (Department of Food Science and Technology, Pukyong National University)
Shin, Hye-Young (Department of Marine Food Science and Technology, Gangneung-Wonju National University)
Shin, Joong Ho (Department of Industry 4.0 Convergence Bionics Engineering, Pukyong National University)
Jung, Won-Kyo (Department of Industry 4.0 Convergence Bionics Engineering, Pukyong National University)
Oh, Junghwan (Department of Industry 4.0 Convergence Bionics Engineering, Pukyong National University)
Kim, Young-Mog (Department of Food Science and Technology, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.54, no.6, 2021 , pp. 912-917 More about this Journal
Abstract
We investigated the distribution, molecular characteristics, and antimicrobial resistance of Vibrio vulnificus isolated from seawater at Gadeok Island, the Republic of Korea between June to October. Interestingly no isolates were detected between December to February. The detection rate of V. vulnificus was high (80-100%) from July to September 2019 and from June to September 2020. This coincided with the relatively low salinity of the seawater, which ranged from 7.8-29.9 practical salinity units for that period. Additionally, V. vulnificus had a high detection rate at sampling stations near the Nakdong river. The detection rates of virulence genes, such as vvhA, viuB, and vcgC, among the isolates were 97.1%, 44.1%, and 57.4% in 2019 and 100%, 43.0%, and 50.0% in 2020, respectively. Notably, viuB and vcgC were detected in V. vulnificus isolated between June to October when water temperature was above 20℃. The antimicrobial susceptibility analysis of 80 isolates revealed that most of the strains were susceptible to most antimicrobial agents. However, some isolates showed intermediate resistance to cefepime (18.8%), cefoxitin (58.8%), and erythromycin (22.5%). Of note, 3.8% of the tested strains were resistant to cefoxitin. The minimum inhibitory concentration of highly cefoxitin-resistant strains was determined to be less than 32 ㎍/mL.
Keywords
Antimicrobial resistance; Vibrio vulnificus; Virulence genes;
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