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http://dx.doi.org/10.5657/KFAS.2019.0035

Protective Effects of Antioxidant Active Fractions Derived from the Edible Seaweed Hizikia fusiformis in Oxidatively Stressed Human Dermal Fibroblasts  

Cui, Yong Ri (Department of Marine Life Science, Jeju National University)
Kim, Hyun-Soo (Department of Marine Life Science, Jeju National University)
Je, Jun-Geon (Department of Marine Life Science, Jeju National University)
Wang, Lei (Department of Marine Life Science, Jeju National University)
Oh, Jae-Young (Department of Marine Life Science, Jeju National University)
Jia, Liu (Qingdao KangMaiChen Biotechnology Co., Ltd. The Institute of Food Research)
Jeon, You-Jin (Department of Marine Life Science, Jeju National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.52, no.1, 2019 , pp. 35-42 More about this Journal
Abstract
We investigated the protective effects of antioxidant fractions from a 70% ethanolic extract of Hizikia fusiformis in human dermal fibroblasts (HDFs). Powdered H. fusiformis was extracted with 70% ethanol and then partitioned into three fractions according to polarity using n-hexane (HFH), chloroform (HFC), and ethyl acetate (HFEA). Antioxidant activity was observed in HFEA at 0.66 mg/mL based on the half maximal inhibitory concentration ($IC_{50}$) of 1,1-diphenyl-2-picrylhydrazyl (DPPH), and at 0.24 mg/mL based on alkyl radical scavenging. The protective effects of the HFEA antioxidant fraction against 2,2-azobis-(2-amidinopropane) dihydrochloride (AAPH)-damaged HDFs and the expression of Type I procollagen in HDFs were examined. HFEA caused the proliferation of HDFs with and without AAPH treatment and protected against AAPH damage to HDFs in a dose-dependent manner ($50-200{\mu}g/mL$). This implies that the antioxidant properties of the fractions depended on their proliferative and protective effects. The HFEA antioxidant fraction had significant effects and caused the dose-dependent expression of Type I procollagen, an important anti-wrinkle protein, in HDFs. In conclusion, antioxidant substances in H. fusiformis were found in the ethyl acetate fraction, and the resulting HFEA may have cosmetic applications.
Keywords
Hizikia fusiformis; Human dermal fibroblasts (HDF); Antioxidant; Cosmetic;
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Times Cited By KSCI : 6  (Citation Analysis)
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