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http://dx.doi.org/10.5657/kfas.2003.36.1.030

Purification of a Bradykinin-Related Peptide from the Skin of Hagfish. Eptatretus burgeri  

SHIN Mi Jung (Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyong National University)
KIM Eun Jung (Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyong National University)
KIM Chan-Hee (Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyong National University)
GO Hye-Jin (Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyong National University)
KIM In Hae (Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyong National University)
RYU Hong-Soo (Department Food and Life Science, College of Fisheries Science, Pukyong National University)
Huh Min-Do (Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University)
CHUNG Joon-Ki (Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University)
PARK Nam Gyu (Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.36, no.1, 2003 , pp. 30-34 More about this Journal
Abstract
A hagfish bradykinin(BK)-related peptide was isolated and characterized from the skin of hagfish, Eptatretus burgeri. The hagfish BK with a molecular mass of 875.8 Da was purified to a homogeneity using $C_{18}$ reverse-phase and cation-exchange high performance liquid chromatography. The primary structure of the hagfish BK was determined as Gly-Thr-Ala-Gly-Ile-Gly-Pro-Phe-Arg by a combination of an automated amino acid sequencing and MALDI-TOF mass spectrometry. This amino acid sequence contains five substitutions $(Arg^1{\rightarrow}Gly,\;Pro^2{\rightarrow}Thr,\;Pro^3{\rightarrow}Ala,\;Phe^5{\rightarrow}Ile,\;Ser^6{\rightarrow}Gly)$ compared with that of mammalian BK. The hagfish BK showed a contractile action on the intestine of hagfish, Eptatretus burgeri. The threshold concentration of hagfish BK was around $10^{-11}\;M.$
Keywords
Hagfish bradykinin (BK); Purification; Contractile action;
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1 Werle, E., K Hochstrasser and I. Trautschold. 1966. Studies of bovine plasma kininogen, ornitho-kallikrein and ornitho-kini. In: Erdos E. Bace N., Sicuteri F., Wilde, AF., editors. Hypotensive peptides, New York: Springer-verlag, pp. 105-115
2 Park, N.G., J-K. Seo, H-J. Ku, S. Lee, G. Sugihara, K-H. Kim, J-S. Park and S-W. Kang. 1997. Conformation and biological activity of mastoparan B and its analogs I. Bull. Korean Chem. Soc. 18, 50-56
3 Platzack, B. and J.M. Conlon. 1997. Purification, structural characterization and cardiovascular activity of cod bradykinins. Am. J. Physiol., 272, R71O-717
4 Regoli, D., A Rizzi, G. Calo, S. Nsa Allogho and F. Gobeil. 1997. B1 and B2 kinin receptors in various species. Immunopharmacology, 36, 143-147   DOI   ScienceOn
5 Regoli, D. and J. Barabe. 1980. Pharmacology of bradykinin and related kinins. Pharmacol. Rev., 32, 1-46
6 Rodeghini, R., G. Falconieri Erspamer, C. Severini and M. Simmaco. 1989. Biogenic amines and active peptides in extracts of the skin of thirty-two european amphibian species. Comp. Biochem. Physiol. [C], 94, 455-460   DOI   ScienceOn
7 Roseghini, R., G. Falconier Erspamer and C. Severini. 1988. Biogenic amines and active peptides in the skin of fifty-two african amphibian species other than bufonids. Comp. Biochem. Physiol. [C], 91, 281-286   DOI   ScienceOn
8 Schluter, H., D. Mentrup, I. Grpb, H. E. Meyer, B. Spengler, R Kaufmann and W. Zidek. 1997. Identification of endogenous Des-$Arg^{9}$-[$Hyp^{3}$]-bradykinin in human plasma with post-source-decay matrix-assisted laser desorption/ionization mass spectrometry. Anal. Biochem., 246, 15-19   DOI   ScienceOn
9 Seki, T., I. Miwa, T. Nakajima and E.G. Erdos. 1973. Kallikrein-kinin system in nonmammalian blood: evolution aspects. Am. J. Physiol., 224, 1425-1430
10 Stewart, J.M. 1979. Chemistry and biologic activity of peptides related to bradykinin. Handb. Exp. Pharmacol., 25, 227-272
11 Conlon, J.M., B. Platzack, L.E. Marra, J.H. Youson and K.R. Olson. 1995. Isolation and biological activity of [$Trp^{5}$] bradykinin from the plasma of the phylogenetically acient fish, the bowfin and the longnosed gar. Peptides, 16, 485-489   DOI   ScienceOn
12 Conlon, J.M., J.C. Le Mevel, D. Conklin, L. Weaver, DW. Duff and K.R. Olson. 1996. Isolation and cardiovascular activity of a second bradykinin-related peptide ([$Arg^{0}$, $Trp^{5}$, $Leu^{8}$]-bradykinin) from trout. Peptides, 17, 531-537   DOI   ScienceOn
13 Erdos, E.G., I. Miwa and W.J. Graham. 1967. Studies on the evolution of plasma kinins: reptilian and avian blood. Life Sci., 6, 2433-2439   DOI   ScienceOn
14 Geppetti, P. 1993. Sensory neuropeptide release by bradykinin: mechanisms and pathophysiological implications. Regul. Pep., 47, 1-23   DOI   ScienceOn
15 Li, Z., M.P. Smith, D.W. Duff, B.A. Barton, K.R. Olson and J.M. Conlon. Purification and cardiovascular activity of [$Met^{1}$, $Mat^{5}$]-bradykininfrom the plasma of a sturgeon (Acipenseriformes). Peptides, 19, 635-641   DOI   ScienceOn
16 Li, Z., S.M. Secor, V.A. Lance, M. Masii, M. Vallarino and J.M. Conlon. 1998. Characterization of bradykinin-related peptides generated in the plasma of six sarcopterygian species (African lungfish, amphiuma, coachwhip, bullsnake, gila monstar and gray's monitor). Gen. Compo Endocrinol., 112, 108-114   DOI   ScienceOn
17 Lipke, D.W. and K.R. Olson. 1990. Enzyme of the kallikrein-kinin system in rainbow trout. Am. J. Physiol., 258, R501-506
18 Muneoka, Y. and M. Kohayashi. 1992. Comparative aspects of structure and action of molluscan neuropeptides. Experimentia, 48, 448-456   DOI   ScienceOn
19 Clewents, J.A 1994. The human kallikrein gene family: A diversity of expression and function. Mol. Cell. Endocrinol., 99, C1-C6   DOI   ScienceOn
20 Comeau, S., V.A. Lance, J.W. Hicks and J.M. Conlon. 1992. Purification and biological activity of alligator bradykinin. Am. J. Physiol., 263, R400-R404
21 Conlon, J.M., D.D. Smith and J.W. Hicks. 1990. Isolation and biological activity of a novel kinin ([$Thr^{6}$] bradykinin) from the turtle, Pseudemys scripta. Endocrinology, 126, 985-991
22 Conlon, J.M. and V.A. Lance. 1994. Generation of [$AIa^{1}$, $Thr^{6}$] bradykinin in the plasma of a snake, the reticulated python. Gen. Compo Endocrinol., 94, 273-278   DOI   ScienceOn