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http://dx.doi.org/10.4163/jnh.2021.54.6.603

Effect of amaranth seed extracts on glycemic control in HepG2 cells  

Park, So Jin (Department of Food and Nutrition, Graduate School of Wonkwang University)
Park, Jong Kun (Department of Biological Science, Wonkwang University)
Hwang, Eunhee (Department of Food and Nutrition, Wonkwang University)
Publication Information
Journal of Nutrition and Health / v.54, no.6, 2021 , pp. 603-617 More about this Journal
Abstract
Purpose: This study was carried out to investigate the effect of amaranth seed extracts on glycemic regulation in HepG2 cells. The 80% ethanol extracts of amaranth seeds were used to evaluate α-amylase and α-glucosidase activities, cell viability, glucose uptake and messenger RNA (mRNA) expression levels of acetyl-CoA carboxylase (ACC), glucose transporter (GLUT)-2, GLUT-4, insulin receptor substrate (IRS)-1 and IRS-2. Methods: The samples were prepared and divided into 4 groups, including germinated black amaranth (GBA), black amaranth (BA), germinated yellow amaranth (GYA) and yellow amaranth (YA). Glucose hydrolytic enzyme, α-amylase and α-glucosidase activities were examined using a proper protocol. In addition, cell viability was measured by MTT assay. Glucose uptake in cells was measured using an assay kit. The mRNA expression levels of ACC, GLUT-2, GLUT-4, IRS-1 and IRS-2 were measured by reverse transcription polymerase chain reaction. Results: The inhibitory activities of α-amylase and α-glucosidase were highly observed in GBA, followed by BA, GYA and YA. Similar results were observed for glucose. The GBA effect was similar compared to the positive control group. The mRNA expression levels of ACC, GLUT-2, GLUT-4, IRS-1, and IRS-2 were significantly increased. The potential hypoglycemic effects of amaranth seed extracts were observed due to the increase in glucose metabolic enzyme activity, and glucose uptake was mediated through the upregulation of ACC, GLUT-2, GLUT-4, IRS-1, and IRS-2 expression levels. Conclusion: Our findings suggest that the amaranth seed is a potential candidate to prevent a diabetes. The present study demonstrated the possibility of using amaranth seeds, especially GBA and BA for glycemic control.
Keywords
amaranth plant; glycemic control; HepG2 cells;
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1 Kim MU, Cho YJ, Kim JH. Isolation and identification of inhibitory compound from Crataegi fructus on α-amylase and α-glucosidase. J Korean Soc Appl Biol Chem 2007; 50(3): 204-220.
2 Ahn CH, Nam YM, Kim SJ, Yang BW, Kim HC, Ko SK. Inhibitory effects of Ginseng seed oil on α-glucosidase and α-amylase activity. Korean J Pharmacogn 2006; 47: 24-28.
3 Kim JE, Joo SI, Seo JH, Lee SP. Antioxidant and α-glucosidase inhibitory effect of Tartary Buckwheat extract obtained by the treatment of different solvents and enzymes. J Korean Soc Food Sci Nutr 2009; 38(8): 989-995.   DOI
4 Moscato S, Ronca F, Campani D, Danti S. Poly(vinyl alcohol)/gelatin hydrogels cultured with Hep G2 cells as a 3D model of hepatocellular carcinoma: a morphological study. J Funct Biomater 2015; 6(1): 16-32.   DOI
5 Zou C, Wang Y, Shen Z. 2-NBDG as a fluorescent indicator for direct glucose uptake measurement. J Biochem Biophys Methods 2005; 64(3): 207-215.   DOI
6 Liu F, Kim J, Li Y, Liu X, Li J, Chen X. An extract of Lagerstroemia speciosa L. has insulin-like glucose uptake-stimulatory and adipocyte differentiation-inhibitory activities in 3T3-L1 cells. J Nutr 2001; 131(9): 2242-2247.   DOI
7 Cheng FC, Shen SC, Wu JS. Effect of guava (Psidium guajava L.) leaf extract on glucose uptake in rat hepatocytes. J Food Sci 2009; 74(5): H132-H138.   DOI
8 Kang YH, Kim DJ, Kim KK, Lee SM, Choe M. Study of the mechanisms underlying increased glucose absorption in Smilax china L. leaf extract-treated HepG2 cells. J Nutr Health 2014; 47(3): 167-175.   DOI
9 Kim DJ, Kang YH, Kim KK, Kim TW, Park JB, Choe M. Effects of Acanthopanax senticosus water extract on glucose-regulating mechanisms in HepG2 cells. J Korean Soc Food Sci Nutr 2017; 46(5): 552-561.   DOI
10 Stewart MJ, Watson ID. Standard units for expressing drug concentrations in biological fluids. Br J Clin Pharmacol 1983; 16(1): 3-7.   DOI
11 Park CM, Kwak BH, Sharma BR, Rhyu DY. Anti-diabetic effect of Opuntia humifusa stem extract. Korean J Pharmacogn 2012; 43(4): 308-315.
12 Kim TY, Kim SJ, Imm JY. Improvement of blood glucose control in type 2 diabetic db/db mice using Platycodon grandiflorum seed extract. Korean J Food Sci Technol 2020; 52: 81-88.   DOI
13 Kim JM, Cho CS, Kim CJ. Effect of YCT on insulin secretion in RIN-m5F cells. J Korean Orient Med 2010; 31(4): 20-37.
14 Lebovitz HE. α-Glucosidase inhibitors as agents in the treatment of diabetes. Diabetes Res 1998; 6: 132-145.
15 Kim DH. Food chemistry. 3rd ed. Seoul; 2010. p.39-81.
16 Korean Diabetes Association. Greetings [Internet]. Seoul: Korean Diabetes Association; 2021 [cited 2021 Jun 10]. Available from: https://www.diabetes.or.kr/general/intro/index.php.
17 Ministry of Health and Welfare, Korea Centers for Disease Control and Prevention. Korea National Health and Nutrition Examination Survey: Korea Centers for Disease Control and Prevention [Internet]. Cheongju: Korea Centers for Disease Control and Prevention; 2021 [cited 2021 Jun 10]. Available from: https://www.seoulnutri.co.kr/food-db/67.do.
18 Barber MC, Price NT, Travers MT. Structure and regulation of acetyl-CoA carboxylase genes of metazoa. Biochim Biophys Acta 2005; 1733(1): 1-28.   DOI
19 Mueckler M, Thorens B. The SLC2 (GLUT) family of membrane transporters. Mol Aspects Med 2013; 34(2-3): 121-138.   DOI
20 Dela F, Ploug T, Handberg A, Petersen LN, Larsen JJ, Mikines KJ, et al. Physical training increases muscle GLUT4 protein and mRNA in patients with NIDDM. Diabetes 1994; 43(7): 862-865.   DOI
21 Lee YH, White MF. Insulin receptor substrate proteins and diabetes. Arch Pharm Res 2004; 27(4): 361-370.   DOI
22 Ayiecho PO, Singh SP, Thimba D. Popping quality of grain Amaranths. East Afr Agric For J 1988; 54(1-2): 85-89.   DOI
23 Hong SY, Cho KS, Jin YI, Yeon YH, Kim SJ, Nam JH, et al. Comparison of growth characteristics, antioxidant activity and total phenolic contents of Amaranthus species according to the different cultivation regions and varieties in South Korea. Korean J Crop Sci 2014; 59(1): 16-21.   DOI
24 Yang SJ, Lee RH, Hong JH. Physicochemical characteristics and biological activities of rice and Amaranth fermented by Bacillus subtilis KMKW4. J Korean Soc Food Sci Nutr 2005; 44(4): 540-548.   DOI
25 Kim HK, Kim MJ, Cho HY, Kim EK, Shin DH. Antioxidative and anti-diabetic effects of amaranth (Amaranthus esculantus) in streptozotocin-induced diabetic rats. Cell Biochem Funct 2006; 24(3): 195-199.   DOI
26 Ryu JW, Yook CS, Chung SH. Effects of Mori folium ethanol soluble fraction on mRNA expression of glucose transporters, acetyl-CoA carboxylase and leptin. J Pharm Soc Korea 1998; 42(6): 589-597.
27 Ward CW, Lawrence MC. Ligand-induced activation of the insulin receptor: a multi-step process involving structural changes in both the ligand and the receptor. BioEssays 2009; 31(4): 422-434.   DOI
28 Paredes LO, Mora ER. Germination of Amaranth seeds: effects on nutrient composition and color. J Food Sci 1989; 54(3): 761-762.   DOI
29 Kasozi KI, Namubiru S, Safiriyu AA, Ninsiima HI, Nakimbugwe D, Namayanja M, et al. Grain Amaranth is associated with improved hepatic and renal calcium metabolism in type 2 diabetes mellitus of male Wistar rats. Evid Based Complement Alternat Med 2018; 2018: 4098942.
30 Shin DH, Heo HJ, Lee YJ, Kim HK. Amaranth squalene reduces serum and liver lipid levels in rats fed a cholesterol diet. Br J Biomed Sci 2004; 61(1): 11-14.   DOI
31 Yi MR, Kang CH, Bu HJ. Anti-inflammatory and tyrosinase inhibition effects of Amaranth (Amaranthus spp L.) seed extract. Korean J Plant Resour 2017; 30(2): 144-151.   DOI
32 Berghofer E, Schoenlechner R. Grain amaranth. In: Belton P, Taylor J, editors. Pseudocereals and Less Common Cereals. Berlin/Heidelberg: Springer-Verlag; 2002. p.219-260.
33 Jo HJ, Kim JW, Yoon JA, Kim KI, Chung KH, Song BC, et al. Antioxidant activities of Amaranth (Amaranthus spp. L.) flower extracts. Korean J Food Nutr 2014; 27(2): 175-182.   DOI
34 Linus Pauling Institute, Micronutrient Information Center. Flavonoids: disease prevention. Corvallis (OR); 2016. p.201-246.
35 Ogawa S, Fujieda S, Sakata Y, Ishizaki M, Hisamatsu S, Okazaki K. Synthesis and glycosidase inhibitory activity of some N-substituted 6-deoxy-5a-carba-β-DL- and L-galactopyranosylamines. Bioorg Med Chem Lett 2003; 13(20): 3461-3463.   DOI
36 Mckee T. Mckee J. Biochemistry: the molecular basis of life. 3rd ed. New York (NY); 2004. p.142-148.
37 Vichayanrat A, Ploybutr S, Tunlakit M, Watanakejorn P. Efficacy and safety of voglibose in comparison with acarbose in type 2 diabetic patients. Diabetes Res Clin Pract 2002; 55(2): 99-103.   DOI
38 Yamaki K, Mori Y. Evaluation of α-glucosidase inhibitory activity in colored foods: a trial using slope factors of regression curves. J Jpn Soc Food Sci Tech 2006; 53(4): 229-231.   DOI
39 Stockert JC, Horobin RW, Colombo LL, Blazquez-Castro A. Tetrazolium salts and formazan products in cell biology: viability assessment, fluorescence imaging, and labeling perspectives. Acta Histochem 2018; 120(3): 159-167.   DOI
40 Kim HS, Kim TW, Kim DJ, Kim KK, Choe M. Effect of medicinal plant water extracts on expression of anti-diabetic enzymes mRNA. J Korean Soc Food Sci Nutr 2013; 42(7): 1008-1014.   DOI