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http://dx.doi.org/10.4163/jnh.2018.51.3.208

Inhibitory effect of the aqueous extract of a tetraploid 'etteum' variety in Platycodon grandiflorum on degranulation and inflammatory mediator release in RBL-2H3 cells  

Jung, Jae In (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University)
Kim, Hyung Seo (Research Institute of Chamdahanbio, Chamdahanbio Ltd.)
Ji, Han Kyeol (Research Institute of Chamdahanbio, Chamdahanbio Ltd.)
Lee, Hyun Sook (Department of Food Science & Nutrition, Dongseo University)
Lee, Jae-Yong (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University)
Kim, Eun Ji (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University)
Publication Information
Journal of Nutrition and Health / v.51, no.3, 2018 , pp. 208-214 More about this Journal
Abstract
Purpose: Platycodon grandiflorum (a domestic diploid variety, DV-PG) has been used as a food and component of various traditional oriental medicines. Although DV-PG is known to have an anti-allergic effect, little is known about the beneficial health effects of the tetraploid 'Etteum' variety in the Platycodon grandiflorum (TV-PG), which is a recently developed variety. In this study, we investigated the effect of TV-PG on the rat basophilic leukemia mast cell (RBL-2H3)-mediated allergic response. Methods: To examine the effects of TV-PG on the allergic response, RBL-2H3 cells were sensitized with dinitropheny (DNP)-immunoglobin E, treated with various concentrations of TV-PG, and challenged with DNP-human serum albumin. We estimated cell granulation by measuring the release of ${\beta}$-hexosaminidase and production of inflammatory mediators by ELISA. Results: TV-PG had no effect on the proliferation or cytotoxicity of RBL-2H3 cells within the concentration range of 0 to $200{\mu}g/mL$. TV-PG inhibited degranulation of RBL-2H3 cells by antigen stimulation in a dose-dependent manner. TV-PG also suppressed the production of inflammatory cytokines and mediators such as interleukin-4, tumor necrosis $factor-{\alpha}$, prostagladin E2, and leukotriene B4 in RBL-2H3 cells by antigen stimulation. Conclusion: These results indicate that TV-PG exhibits anti-allergic activity via inhibition of degranulation as well as suppression of inflammatory mediators and cytokine release. These findings suggest that TV-PG may have potential as a preventive and therapeutic agent for the treatment of various allergic diseases.
Keywords
functional food; Platycodon grandiflorum; allergy; mast cell; inflammation mediators;
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Times Cited By KSCI : 4  (Citation Analysis)
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