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http://dx.doi.org/10.4163/jnh.2015.48.4.310

Effects of natural eggshell membrane (NEM) on monosodium iodoacetate-induced arthritis in rats  

Sim, Boo Yong (Traditional and Biomedical Research Center (TBRC-RIC), Daejeon University)
Bak, Ji Won (Traditional and Biomedical Research Center (TBRC-RIC), Daejeon University)
Lee, Hae Jin (Traditional and Biomedical Research Center (TBRC-RIC), Daejeon University)
Jun, Ji Ae (Traditional and Biomedical Research Center (TBRC-RIC), Daejeon University)
Choi, Hak Joo (Traditional and Biomedical Research Center (TBRC-RIC), Daejeon University)
Kwon, Chang Ju (Ju Yeong NS Co., Ltd.)
Kim, Hwa Young (Ju Yeong NS Co., Ltd.)
Ruff, Kevin J. (Scientific & Regulatory Affairs, ESM Technologies, LLC)
Brandt, Karsten (Human Nutrition, Stratum Nutrition)
Kim, Dong Hee (Traditional and Biomedical Research Center (TBRC-RIC), Daejeon University)
Publication Information
Journal of Nutrition and Health / v.48, no.4, 2015 , pp. 310-318 More about this Journal
Abstract
Purpose: The aim of this study is to investigate anti-arthritis activity using natural eggshell membrane (NEM). Methods: NEM was administered at 52 mg/kg, 200 mg/kg, and 400 mg/kg to SD-Rat, where arthritis was induced by monosodium iodoacetate (MIA) at 3 mg. NO production in serum was measured using Griess reagent. Cytokines including IL-$1{\beta}$, and IL-6 were measured by Luminex and $PGE_2$, MMP-2, MMP-9, TIMP-1, $LTB_4$, and hs-CRP were measured by ELISA. The cartilage of patella volume was examined and 3-D high-resolution reconstructions of the cartilage of patella were obtained using a Micro-CT system. Results: Production of NO, IL-$1{\beta}$, IL-6, $PGE_2$, MMP-2, MMP-9, TIMP-1, $LTB_4$, and hs-CRP in serum was decreased, respectively, in comparison with control. The cartilage of patella volume increased significantly. In addition, the NEM group showed a decrease in the cartilage of patella, synovial membrane, and transformation of fibrous tissue. Conclusion: The results for NEM showed significant anti-arthritis activity. These results may be developed as a raw material for new health food to ease the symptoms mentioned above.
Keywords
osteoarthritis; anti-Inflammatory; NEM; MIA; health food;
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